Diagnosis of AA-Amyloidosis Using Monoclonal Antibodies: Application to Paraffin and Ultrathin Sections as Well as to Urinary Sediments

  • Reinhold P. Linke
  • Dieter Huhn
  • Walter B. J. Nathrath


In order to establish a technique that is suitable to exactly diagnose all AA-type amyloid diseases for clinico-pathologic routine use, a series of 4 murine monoclonal antibodies directed against human AA-protein+, i.e. mc1, mc4, mc8 and mc20, were employed to examine their performance on routinely fixed and in paraffin and in plastic embedded tissue sections. It is demonstrated here, that
  1. (a)

    all 114 AA-amyloid cases have clearly been recognized in paraffin sections among 184 cases with amyloidosis examined. Neither false positive nor false negative cases were noted; thus, the staining was highly specific;

  2. (b)

    AA-fibrils could be identified with mcl and mc20 in maraglas-embedded ultrathin sections virtually without background staining.

  3. (c)

    also non-human AA from gazella, hare, dog and horse (generalized form) could be identified in paraffin sections, with mc4.

  4. (d)

    AA-antigenic material could be detected in urinary sediments in AA-amyloidosis in some patiens, but in general not in contols.


These observations demonstrate, that monoclonal antibodies are highly specific in establishing the unequivocal diagnosis of AA-amyloidosis on the light and electron microscopic level and may open the possibility to diagnose AA-amyloidosis from the urinary sediments in progressive and destructive glomerular amyloidosis.


Familial Mediterranean Fever Carpal Tunnel Syndrome Amyloid Fibril Juvenile Rheumatoid Arthritis Urinary Sediment 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Reinhold P. Linke
    • 1
  • Dieter Huhn
    • 2
  • Walter B. J. Nathrath
    • 3
  1. 1.Institut für ImmunologieMünchenGermany
  2. 2.Abt. HämatologieGesellschaft für Strahlen-u. UmweltforschungMünchenGermany
  3. 3.Pathologisches InstitutMünchenGermany

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