Diagnosis of AA-Amyloidosis Using Monoclonal Antibodies: Application to Paraffin and Ultrathin Sections as Well as to Urinary Sediments
all 114 AA-amyloid cases have clearly been recognized in paraffin sections among 184 cases with amyloidosis examined. Neither false positive nor false negative cases were noted; thus, the staining was highly specific;
AA-fibrils could be identified with mcl and mc20 in maraglas-embedded ultrathin sections virtually without background staining.
also non-human AA from gazella, hare, dog and horse (generalized form) could be identified in paraffin sections, with mc4.
AA-antigenic material could be detected in urinary sediments in AA-amyloidosis in some patiens, but in general not in contols.
These observations demonstrate, that monoclonal antibodies are highly specific in establishing the unequivocal diagnosis of AA-amyloidosis on the light and electron microscopic level and may open the possibility to diagnose AA-amyloidosis from the urinary sediments in progressive and destructive glomerular amyloidosis.
KeywordsFamilial Mediterranean Fever Carpal Tunnel Syndrome Amyloid Fibril Juvenile Rheumatoid Arthritis Urinary Sediment
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