The Amino Acid Sequence of a Carbohydrate-Containing Immuno-Globulin-Light-Chain-Type Amyloid Fibril Protein (AL)
The amino acid sequence of AL chain So124 was established from N-terminal analyses of the protein itself and on peptides derived after tryptic digestion, digestion with protease V8, digestion with thermolysin and cleavage with BNPS-skatole. The protein was found to consist of 103 amino acid residues and had an oligosaccharide chain linked to an asparagine residue in position 72. The AL chain was most homologous to the variable region of k-type subgroup III.
KeywordsAmyloid Fibril Tryptic Digestion Complete Amino Acid Sequence Immunoglobulin Light Chain High Performance Liquid Chromato
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- 5.K. Sletten, P. Westermark and G. Husby, Structural studies of the variable region of immunoglobulin light-chaintype amyloid fibril proteins, in: “Amyloidosis”, G.G. Glenner, E.F. Osserman, E.P. Benditt, E. Calkins A.S. Cohen and D. Zucker-Franklin, eds., Plenum Press, New York (1986).Google Scholar
- 7.K. Sletten, J. Natvig, G. Husby and J. Juul, The complete amino acid sequence of a prototype immunoglobulin-light-chain-type amyloid-fibril protein AR, Biochem.J. 195:561 (1981).Google Scholar
- 8.B. Frangione, T. Moloshok and A. Solomon, Primary structure of the variable region of a human VI light chain: Bence Jones protein SUT, J.Immunol. 131:2490 (1983).Google Scholar
- 10.C.H. Bolton, J.R. Clamp and L. Hough, The use of gasliquid chromatography in investigations on glycoproteins, Biochem.J. 96:5c (1965).Google Scholar
- 13.W.C. Mahoney and M.A. Hermodson, Separation of large denatured peptides by reverse phase high performance liquid chromatography, J.Biol.Chem. 261:8442 (1986).Google Scholar
- 15.E.A. Kabat, T.T. Wu, M. Reid-Miller, H.M. Perry and K.S. Gottesman, 1987 Sequences of Proteins of Immunological Interest, U.S. Dept. of Health and Human Services, Public Health Service N.I.H., Bethesda.Google Scholar
- 16.E. Holm, K. Sletten and G. Husby, Structural studies of a carbohydrate-containing immunoglobulin-λ-light-chain amyloid-fibril protein (AL) of variable subgroup III, Biochem.J. 239:545 (1986).Google Scholar