Experimental Amyloidosis of Rabbit Induced by Saponin
It is well known that experimental amyloidosis is induced by injection of various materials, such as casein, Escherichia coli endotoxin, Candida albicans and so on1. Saponin, a glycoside mixture of the white soaproot of Gypsophilia species, was also found out to be one of the amyloidogenic agents2.The present paper describes the light and electron microscopic appearances of amyloidosis in the kidney and other organs induced by saponin. The experiment was also undertaken to clarify the initial change in the kidney by sequential biopsies. Furthermore, major protein constituent of the amyloid substance in these animals was purified and its amino acid composition and residue sequence were analyzed.
KeywordsAmyloid Deposit Amyloid Fibril Amyloid Protein Renal Amyloidosis Residue Sequence
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- 1.A. S. Cohen and T. Shirahama, Animal model for human disease, Am. J. Pathol.Google Scholar
- 2.M. Fujiwara, M. Watanabe, M. Hotchi and T. Nasu, Experimental research as to membranous lypodystrophy (Nasu disease), Tr. Sac. Pathol. Jpn. 71:149 (1982).Google Scholar
- 3.J. Cui, M. Hayama and M. Hotchi, A new method of amyloid staining-Phenol congo red, Pathol. Clin. Med. 4:1229 (1986). (In Japanese)Google Scholar
- 6.N. Takahashi, T. Takayasu, T. Shinoda, S. Ito, T. Okuyama and A. Shimizu, Amino acid sequence of a lambda bence jones protein from a case of primary amyloidosis, Biomed. Res. 1:321 (1980).Google Scholar
- 7.K. Kimura, I. Kihara and S. Kitamura, The five structure of glomerular epithelial cells in experimental renal amyloidosis, Acta. Pathol. Jpn. 24:779 (1974).Google Scholar
- 8.A. S. Cohen, E. Calkins and I. L. Charles, Analysis of histology and staining reactions of casein-induced amyloidosis in the rabbit, Am. J. Pathol. 35:971 (1959).Google Scholar