Ultrastructural Study of the Spleen in Experimental Murine Amyloidosis Using the Protein A-Gold Immunocytochemical Technique
Following a single administration of amyloid-enfancing factor and daily casein injections, the early amyloid deposition in the murine spleen was studied ultrastructurally using the protein A-gold immunocytochemical technique. At 2 days after the injections, a tiny amount of amyloid deposits was detected not only in the vicinity of the border of the marginal zone and white pulp (perifollicular area) but also in the white pulp apart from the border. These amyloid deposits were located mainly extracellularly in a random fashion along with collagen or reticular fibers. Some amyloid fibrils were in close contact with cytoplasmic processes of histiocytic cells which had a large number of lysosomal dense bodies in their cytoplasm. By protein A-gold immunocytochemical technique, gold particles were labeled not only in oval to fusiform dense bodies of the histiocytic cells containing fine fibrillar structures but also in a few electron-dense granules in which any fibrillar structures were not discernible. No topographic relationship was noted between amyloid fibrils and lymphoid or reticular cells, the basement membrane of vessels in the marginal zone, or white pulp.
From these results, it is confirmed that the early amyloid deposits in the spleen occur mainly extracellularly and that the histiocytic cells in the perifollicular area and white pulp play an essential role in the formation of the amyloid fibrils in the organ.
KeywordsKupffer Cell Marginal Zone Amyloid Fibril Dense Body Reticular Cell
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