Immunolocalization of Heparan Sulfate Proteoglycans to AA Amyloid Deposition Sites in Spleen and Liver During Experimental Amyloidosis
Our previous histochemical studies have demonstrated that during experimental AA amyloidosis a close time relationship exists between initial amyloid and glycosaminoglycan (GAG) accumulation, regardless of the induction protocol used, tissue concerned or length of induction (13). Use of the Alcian blue — magnesium chloride staining technique (11) additionally suggested that highly sulfated GAGs were involved (13), Recent qualitative and quantitative biochemical investigations (14) have confirmed the close temporal relationship between initial amyloid and GAG deposition, and have identified heparan sulfate and heparin as the two major GAGs which accumulate in mouse spleen during experimental AA amyloidosis, a process which did not occur during acute inflammation.
KeywordsHeparan Sulfate Alcian Blue Amyloid Fibril Heparan Sulfate Proteoglycan Sulfated GAGs
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