Immunohistochemical Classification of Amyloidosis and Immunohistochemistry of Fibrillar Nephropathy on Routinely Processed Plastic-Embedded Tissues
Immunohistochemical amyloid classification was performed on plastic embedded renal biopsies tissue of 18 patients with generalized amyloidosis using two different techniques: unlabeled peroxidase method to stain hydroxyethyl-methacrylate embedded tissues at light microscopy and protein A-gold technique to stain epon-araldite routinely embedded tissues for immunoelectron microscopy. The standard processing of tissues included fixation in 2.5% glutaraldehyde and postfixation in 1% osmium tetroxide. No etching was used before immunogold stainings. Antibodies against the AA-*, Aλ-, Aκ-, AF-amyloid fibril proteins were used. The amyloid classification achieved with these two methods was concordant without exception and was closely correlated to clinical diagnosis. The background staining was virtually absent. Due to optimal preservation of morphological details, the antigenic localization of the amyloid fibrils was particularly accurate. With these methods also 2 cases with fibrillar nephro-pathy were examined, in which no reaction was obtained with the anti-amyloid antibodies tested.
KeywordsAmyloid Fibril Immunoelectron Microscopy Immunogold Staining Amyloid Fibril Protein Renal Biopsy Tissue
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