Induced AA Amyloid in Hamster: On the Amyloid Enhancing Factor and Protein AA-Cross Reacting Components of Intermediate Molecular Weight
In the pathogenesis of AA-amyloidosis serum amyloid protein A (SAA) and the amyloid enhancing factor (AEF) are thought to play essential roles1. SAA is an acute phase reactant produced in hepatocytes stimulated by cytokines released by inflammatory cells2,3. SAA is present in the bloodstream mainly as a part of high density lipoprotein. of its structure, amino acid composition and DNA sequence in several species a great deal is known already. In contrast, it is not known where the AEF is produced or by what cells. Furthermore, its exact chemical nature is unknown. In the experimental murine model AEF has been described as a (glyco-) protein, because of its inactivation by proteolytic enzymes4,5. However, upto now the AEF resists every attempt to ascribe its activity to one specific protein5. Moreover, regarding the molecular size of the AEF, different data are given in the literature, depending on varying research groups and the source of the AEF, ranging from 10,000 to over 400,000 dalton4,5,6,7,8. The suggestion was made that AEF could be a small molecule that is easily complexing with itself or normal tissue components1,8.
KeywordsFiltration Electrophoresis Trypsin Fibril Amyloidosis
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