Reactivity of Immunoglobulins on Amyloid in Lichenoid and Macular Amyloidoses with Epidermal Keratin

  • Kaoru Ito
  • Ken Hashimoto


IgG and IgM deposits have been found on amyloid deposits in lichen amyloidosus (LA) and macular amyloidosis (MA) (MacDonald et al., 1977; Norén et al., 1983). On the other hand, ultrastructural examinations have demonstrated close arrangement of degenerated apoptotic keratinocytes and amyloid fibrils (Kumakiri and Hashimoto, 1979). Furthermore, the possible origin of amyloid protein from keratin has been supported by intense immunological investigations using polyclonal and monoclonal anti-keratin antibodies. It has been shown that amyloid deposits in LA and MA are positive for these anti-keratin antibodies (Kobayashi and Hashimoto, 1983; Hashimoto, 1984; Eto et al., 1984.)


Amyloid Deposit Amyloid Protein Glycine Buffer Nitrocellulose Paper Keratin Protein 
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  1. Abrass, C. K., 1986, Evaluation of sequential glomerular eluates from rats with Heymann nephritis, J Immunol., 137:530.Google Scholar
  2. Baker, K. W., and Habowsky, J. E. J., 1983, EDTA separation and ATPase Langerhans cell staining in the mouse epidermis, J Invest Dermatol., 80:104.CrossRefGoogle Scholar
  3. Bennett, F. C., and Yeoman, L.C., 1983, An improved procedure for the ‘dot immunobinding’ analysis of hybridoma supernatants, J. Immunol Methods., 61:201.CrossRefGoogle Scholar
  4. Eichner, R., Bonitz, P., and Sun, T.-T., 1984., Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expresseion, J Cell Biol., 98:1388.CrossRefGoogle Scholar
  5. Eto, H., Hashimoto, X., Kobayashi, H., Fukaya, T., Matsumoto, M., and Sun, T.-T., 1984, Differential staining of cytoid bodies and skin-limited amyloids with monoclonal anti-keratin antibodies, Am J Pathol., 116:473.Google Scholar
  6. Eto, H., Hashimoto, K., Kobayashi, H., Matsumoto, M., Kanzaki, T., Mehregan, A. H., and Weiss, R. A., 1985, Monoclonal antikeratin antibody: Production, characterization, and immunohistochemical application, J Invest Dermatol., 84:404.CrossRefGoogle Scholar
  7. Feltkamp, T. E. W., and Boode, J. H., 1970, Elution of antibodies from biopsy tissue, J Clin Pathol., 23:629.CrossRefGoogle Scholar
  8. Grubauer, G., Romani, N., Kofler, H., Stanzl, U., Fritsch, P., and Hintner, H., 1986, Apoptotic keratin bodies as autoantigen causing the production of IgM-anti-keratin intermediate filament autoantibodies, J Invest Dermatol., 87:466.CrossRefGoogle Scholar
  9. Hashimoto, K., 1984, Progress on cutaneous amyloidoses, J Invest Dermatol., 82:1.CrossRefGoogle Scholar
  10. Hawkes, R., Niday, E., and Gordon, J., 1982, A dot-immunobinding assay for monoclonal and other antibodies, Anal Biochem., 119:142CrossRefGoogle Scholar
  11. Hintner, H., Steinert, P. M., and Lawley, T. J., 1983, Human upper epidermal cytoplasmic antibodies are directed against keratin intermediate filament proteins, J Clin Invest., 71:1344.CrossRefGoogle Scholar
  12. Hintner, H., Neises, G. R., and Lawley, T. J., 1985, Immunologic properties of enzymatically degraded human keratin intermediate filaments, J Invest Dermatol., 84:108.CrossRefGoogle Scholar
  13. Hintner, H., Romani, N., Stanzl, U., Grubauer, G., Fritsch, P., and Lawley, T. J., 1987, Phagocytosis of keratin filament aggregates following opsonization with IgG-anti-keratin filament autoantibodies, J Invest Dermatol., 88:176.CrossRefGoogle Scholar
  14. Ito, K., Hashimoto, K., and Handel, A., 1987, Naturally occurring anti-keratin autoantibodies in human and murine sera are age-related, J Invest Dermatol., 88:4–95.CrossRefGoogle Scholar
  15. Iwatsuki, K., Viac, J., Reano, A., Morera, A., Staquet, M.-J., Thivolet, J., and Monier, J.-C., 1986, Comparative studies on naturally occurring antikeratin antibodies in human sera, J Invest Dermatol., 87:179.CrossRefGoogle Scholar
  16. Kobayashi, H. and Hashimoto, K., 1983, Amyloidogenesis in organ-limited cutaneous amyloidosis: An antigenic identity between epidermal keratin and skin amyloid, J Invest Dermatol., 80:66.CrossRefGoogle Scholar
  17. Kumakiri M., and Hashimoto, K., 1979, Histogenesis of primary localized cutaneous amyloidosis: Sequential change of epidermal keratinocytes to amyloid via filamentous degeneration, J Invest Dermatol., 73:150.CrossRefGoogle Scholar
  18. Laemmli, U. K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature., 227:680.CrossRefGoogle Scholar
  19. MacDonald, D. M., Black, M. M., and Ramnarain, N., 1977, Immunofluorescence studies in primary localized cutaneous amyloidosis, Br J Dermatol., 96:635.CrossRefGoogle Scholar
  20. MacDonald, D. M., Fergin, P. E., and Black, M. M., 1980, Localized cutaneous amyloidosis. A clinical review of 100 cases including immunofluorescent studies, in: “Amyloid and Amyloidosis,” G. G. Glenner, P. Pinho e Costa, and A. Falcão de Freitas, ed., Excerpta Medica, Amsterdam.Google Scholar
  21. Mukai, H., Eto, H., and Nishiyama, S., 1985, Immunoglobulins and complement in skin-limited amyloids can be depleted by chemical pre-treatments, J Invest Dermatol., 84:307.Google Scholar
  22. Norén, P., Westermark, P., Cornwell III, G. G., and Murdoch, W., 1983, Immunofluorescence and histochemical studies of localized cutaneous amyloidosis, Br J Dermatol., 108:277.CrossRefGoogle Scholar
  23. Serre, G., Vincent, C., Viraben, R., and Soleilhavoup, J.-P., 1987, Natural IgM and IgG autoantibodies to epidermal keratins in normal human sera. I: ELISA-titration, immunofluorescence study, J Invest Dermatol., 88:21.CrossRefGoogle Scholar
  24. Towbin, H., Staehelin, T., and Gordon, J., 1979, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications, Proc Natl Acad Sci USA., 76:4–350.CrossRefGoogle Scholar
  25. Weiss, R. A., Eichner, R., and Sun, T.-T., 1984, Monoclonal antibody analysis of keratin expression in epidermal diseases: A 4-8-amd 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes, J. Cell Biol., 98:1397.CrossRefGoogle Scholar
  26. Woodroffe, A. J., and Wilson, C. B., 1977, An evaluation of elution techniques in the study of immune complex glomerulonephritis, J Immunol., 118:1788.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Kaoru Ito
    • 1
    • 2
  • Ken Hashimoto
    • 1
    • 2
  1. 1.Department of DermatologyNiigata University School of MedicineNiigataJapan
  2. 2.Department of DermatologyWayne State University School of MedicineDetroitUSA

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