In Vitro Formation of Amyloid Fibrils from Murine Serum Amyloid a Protein (SAA)

  • Satoshi Baba
  • Katsutoshi Miura
  • Michio Fujie
  • Haruyuki Shirasawa


An attempt to create amyloid fibrils in vitro from murine serum amyloid A protein (SAA) was made. The purified SAA was cleaved by cyanogen bromide and a peptide corresponding to the residues 24-76 of it was isolated. After dialysis against distilled water for 2 days, the precipitate of the peptide showed spontaneous formation of fibrils which were congophilic with green birefringence under polarized light and were by electron microscopy approximately 10 nm wide and indeterminate in length.


Amyloid Fibril Phosphotungstic Acid Cyanogen Bromide Green Birefringence Similar Primary Structures13 


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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Satoshi Baba
    • 2
  • Katsutoshi Miura
    • 2
  • Michio Fujie
    • 1
  • Haruyuki Shirasawa
    • 2
  1. 1.Equipment CenterHamamatsu University School of MedicineHamamatsuJapan
  2. 2.Department of PathologyHamamatsu University School of MedicineHamamatsuJapan

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