In Vitro Formation of Amyloid Fibrils from Murine Serum Amyloid a Protein (SAA)
An attempt to create amyloid fibrils in vitro from murine serum amyloid A protein (SAA) was made. The purified SAA was cleaved by cyanogen bromide and a peptide corresponding to the residues 24-76 of it was isolated. After dialysis against distilled water for 2 days, the precipitate of the peptide showed spontaneous formation of fibrils which were congophilic with green birefringence under polarized light and were by electron microscopy approximately 10 nm wide and indeterminate in length.
KeywordsAmyloid Fibril Phosphotungstic Acid Cyanogen Bromide Green Birefringence Similar Primary Structures13
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