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Immunohistochemical Studies of Age-Associated Amyloid Deposition in the Joint of Senescence-Accelerated Mouse (SAM)

  • Katsuji Shimizu
  • Keiichi Higuchi
  • Mutsumi Matsushita
  • Takao Yamamuro
  • Toshio Takeda
Chapter

Abstract

The senescence accelerated mouse (SAM) is a murine model of accelerated senescence and consists of the senescence-accelerated prone mouse (SAM-P) and senescence-accelerated resistant mouse (SAM-R), the latter of which shows normal ageing characteristics. SAM shows high incidence of age-associated microscopic amyloid deposition in synovial joints and intervertebral discs and the lesion is histologically quite similar to that of humans. The amyloid fibril protein of these mice is well characterized as a murine systemic senile amyloid (ASSAM). Twenty SAM-P and three SAM-R mice were used for this immunohistochemical study. Synovial joints and intervertebral discs were stained by immunoperoxidase method (PAP) using anti-ASSAM and anti-mouse AA antibodies and compared with birefringence in Congo-red stained section. Positive staining was observed in annulus fibrosus of the intervertebral discs, blood vessels, synovia and on the surfaces of the meniscus and articular cartilage, exactly at the same site where green birefringence in Congo-red staining was observed. Both ASSAM and AA existed in the articular structures of SAM and the incidence of AA was significantly correlated with systemic signs of inflammation at autopsy. Among blood vessel, synovium and articular cartilage, there was no one tissue where amyloid deposited earlier than others.

Keywords

Articular Cartilage Intervertebral Disc Amyloid Deposition Amyloid Fibril Synovial Joint 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Katsuji Shimizu
    • 1
  • Keiichi Higuchi
    • 2
  • Mutsumi Matsushita
    • 1
  • Takao Yamamuro
    • 1
  • Toshio Takeda
    • 2
  1. 1.Department of Orthopaedic SurgeryChest Disease Research Institute Kyoto UniversityKyotoJapan
  2. 2.Department of PathologyChest Disease Research Institute Kyoto UniversityKyotoJapan

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