Serum Clearance Rate of Senile Amyloid Related Apolipoprotein A-II is Accelerated with Increasing Age in Senescence Accelerated Mouse (SAM)

  • Hironobu Naiki
  • Keiichi Higuchi
  • Tomonori Yonezu
  • Masanori Hosokawa
  • Toshio Takeda


A unique senile amyloid fibril protein has been isolated from an inbred strain of mice, SAM-P/l (senescence accelerated mouse-prone) (1), established in our laboratory as a model of accelerated senescence (2,3). This amyloid protein, termed “ASSAM”, has a molecular weight of 8,700 and deposits extracellularly, with advancing age, in all tissues, except the brain and bone marrow (1, 4, 5). Using specific antiserum against ASSAM, we found that apo A-II apolipoprotein in mouse high density lipoprotein (HDL) is a serum precursor of murine senile amyloidosis (6,7). Apo A-II deposits as ASSAM without degradation (6) and also decreases in concentration with advancing age (8). We determined the primary structure of ASSAM (9) and also those of apo A-II purified from amyloid resistant slc;ICR mice (10) and SAM-R/1 (senescence accelerated mouse-resistant) (11), and SAM-P/l (11), respectively. Interestingly we found one amino acid substitution at position 5, which was proline in SAM-R/1 and ICR apo A-II, while it was glutamine in SAM-P/l HDL.


High Density Lipoprotein Accelerate Senescence Amyloid Protein Senescence Accelerate Mouse Serum Clearance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    A. Matsumura, K. Higuchi, K. Shimizu, M. Hosokawa, K. Hashimoto, K. Yasuhira and T. Takeda. Lab Invest, 1982 47:270–275Google Scholar
  2. 2.
    T. Takeda, M. Hosokawa, S. Takeshita, M. Irino, K. Higuchi, T. Matsushita, Y. Tomita, K. Yasuhira, H. Hamamoto, K. Shimizu, M. Ishii and T. Yamamuro. Mech Ageing Dev, 1981 17:183–194CrossRefGoogle Scholar
  3. 3.
    M. Hosokawa, R. Kasai, K. Higuchi, S. Takeshita, K. Shimizu, H. Hamamoto, A. Honma, M. Irino, K. Toda, A. Matsumura, M. Matsushita and T. Takeda. Mech Ageing Dev, 1984 26:91–102CrossRefGoogle Scholar
  4. 4.
    K. Higuchi, A. Matsumura, A. Honma, S. Takeshita, K. Hashimoto, M. Hosokawa, K. Yasuhira and T. Takeda. Lab Invest, 1983 48:231–240Google Scholar
  5. 5.
    S. Takeshita, M. Hosokawa, M. Irino, K. Higuchi, K. Shimizu, K. Yasuhira and T. Takeda. Mech Ageing Dev, 1982 20:13–23CrossRefGoogle Scholar
  6. 6.
    K. Higuchi, A. Matsumura, K. Hashimoto, A. Honma, S. Takeshita, M. Hosokawa, K. Yasuhira and T. Takeda. J Exp Med, 1983 158: 1600–1614CrossRefGoogle Scholar
  7. 7.
    K. Higuchi, T. Yonezu, K. Kogishi, A. Matsumura, S. Takeshita, K. Higuchi, A. Kohno, M. Matsushita, M. Hosokawa and T. Takeda. J Biol Chem, 1986 261:12834–12840Google Scholar
  8. 8.
    K. Higuchi, A. Matsumura, A. Honma, K. Toda, S. Takeshita, M. Matsushita, T. Yonezu, M. Hosokawa and T. Takeda. Mech Ageing Dev, 1984 26:311–326CrossRefGoogle Scholar
  9. 9.
    T. Yonezu, K. Higuchi, S. Tsunasawa, S. Takagi, F. Sakiyama and T. Takeda. FEBS Lett, 1986 203:149–152CrossRefGoogle Scholar
  10. 10.
    K. Higuchi, T. Yonezu, S. Tsunasawa, F. Sakiyama and T. Takeda. FFBS Lerr, 1986 207:23–27Google Scholar
  11. 11.
    T. Yonezu, S. Tsunasawa, K. Higuchi, K. Kogishi, H. Naiki, K. Hanada, F. Sakiyama and T. Takeda. Lab Invest, 1987 57: 65–70Google Scholar
  12. 12.
    A. S. McFarlane. Nature, 1958 182:53CrossRefGoogle Scholar
  13. 13.
    B. J. Davis. Ann NY Acad Sei, 1964 121:404–427CrossRefGoogle Scholar
  14. 14.
    J. S. Hoffman and E. P. Benditt. J Clin Invest, 1983 71:926–934CrossRefGoogle Scholar
  15. 15.
    J. S. Parks and L. L. Rudel. Am J Pathol, 1983 112:243–249Google Scholar
  16. 16.
    A. Kohno, T. Yonezu, M. Matsushita, M. Irino, K. Higuchi, K. Higuchi, S. Takeshita, M. Hosokawa and T. Takeda. J Nutr, 1985 115:1259–1266Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Hironobu Naiki
    • 1
  • Keiichi Higuchi
    • 1
  • Tomonori Yonezu
    • 1
  • Masanori Hosokawa
    • 1
  • Toshio Takeda
    • 1
  1. 1.Department of Pathology, Chest Disease Research InstituteKyoto UniversitySakyo-ku, Kyoto 606Japan

Personalised recommendations