Chemical Analysis of Beta2-Microglobulin Derived Amyloid in Patients on Long-Term Hemodialysis

  • Reinhold P. Linke
  • Ursula Kunert
  • Hartmut Lobeck
  • Hannelore Hampl
  • Manfred Eulitz


Amyloid deposits of a patient (KRO) who underwent peritoneal and longterm hemodialysis were chemically, immunochemically and immunohistochemically investigated. The amyloid fibrils from bone marrow were concentrated, dissolved in 80% and separated in 60% formic acid by HPLC according to size. Several different protein peaks were recovered, having molecular weights of approximately 24, 18, 12, and 7–10 kd. The largest fraction represented the void volume peak. All fractions reacted strongly with an antiserum directed against β2m+). N-terminal amino acid sequence analysis showed three different molecules, all derived from β2m: one with the intact N-terminus, a second and a third with either isoleucine in position 6 or serine in position 11 as N-terminus, respectively.

When compared with two additional AB-amyloids that were analysed similarly, two points seem important: (a) in all three cases fragmentation of β2m had occurred, and (b) this fragmentation was not random but in general lysine-specific; this was also the case with one of two AB-protein fragments in patient KRO. These results suggest further, that during the formation of AB-amyloid also limited proteolysis occurs.

Immunohistochemical analysis of vascular amyloid in heart and joint capsule showed antigenic differences when investigated with a panel of anti-amyloid antisera. While in the periarticular tissue only anti-β2m reacted, in heart amyloid both, anti-β2m and anti-AA were reactive. This finding raises the possibility that two different amyloid types can reside in one individual simultaneously. Both amyloids are associated with longterm hemodialysis, but each is induced by probably a different mechanism.


Carpal Tunnel Syndrome Amyloid Deposit Amyloid Fibril Fibril Protein Vascular Amyloid 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Reinhold P. Linke
    • 1
  • Ursula Kunert
    • 1
  • Hartmut Lobeck
    • 2
  • Hannelore Hampl
    • 3
  • Manfred Eulitz
    • 4
  1. 1.Institut für ImmunologieMünchen 2Germany
  2. 2.Institut für PathologieBerlin 19Germany
  3. 3.Kuratorium für HeimdialyseBerlin 12Germany
  4. 4.Abteilung ImmunologieGesellschaft für Strahlen-und UmweltforschungMünchen 2West-Germany

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