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Receptor-Mediated Transport of Cobalamin in Caco-2 Cells: Intracellular Localisation of Transcobalamin II

  • Ian F. Hassan
  • Ian J. Parsons
  • Martin W. Mackay
Part of the NATO ASI Series book series (NSSA, volume 218)

Abstract

A number of in vitro models have been developed to study the absorption of drugs across the intestinal epithelium (Osiescka et al., 1985). The majority of these models do not have the morphological and functional properties of normal adult human epithelial cells. Recently, a human adenocarcinoma cell line (Caco-2) that reproducibly displays properties of differentiated distal ileal epithelial cells has been characterised (Wilson et al., 1989; Hidalgo et al., 1989). When cultured on permeable supports (nitrocellulose filters), in chambers, Caco-2 cells form a confluent monolayer which is morphologically polar with a well developed brush border at their apical surface. The monolayers are functional in the specific and unidirectional transport of taurocholic acid, (Wilson et al., 1989), and the active transport of phenylalanine (Hidalgo and Borchardt, 1988).

Keywords

Vesicular Stomatitis Virus Taurocholic Acid Ficoll Gradient Human Adenocarcinoma Cell Line Polarise Delivery 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this paper

ATP

adenosine 5′-triphosphate

BSA

bovine serum albumin

Cbl

cobalamin

DPM

disintegrations per minute

HEPES

N-2-hydroxyethyl piperazine-N-2-ethane sulphonic acid

IF

intrinsic factor

MES

2-[N-morpholino]ethanesulfonic acid sodium salt

NADH

β-nicotinamide adenine dinucleotide

PBS

phosphate buffered saline

PTA

phosphotungstic acid

RCF

relative centrifugal force

TCA

trichloroacetic acid

TCII

transcobalamin II

TEM

transmission electron micrograph

Tris

Tris(hydroxymethyl)aminomethane-HCl

UDP-galactose

uridine diphospho-d-galactose

VSV

vesicular stomatitis virus

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Ian F. Hassan
    • 1
  • Ian J. Parsons
    • 1
  • Martin W. Mackay
    • 1
  1. 1.Advanced Drug Delivery ResearchCiba-Geigy PharmaceuticalsHorsham, West SussexUK

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