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Passive Absorption of Drugs in Caco-2 Cells

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Pharmaceutical Applications of Cell and Tissue Culture to Drug Transport

Part of the book series: NATO ASI Series ((NSSA,volume 218))

Abstract

In vitro studies on the intestinal absorption of drugs are usually performed on isolated intestinal segments from various experimental animals (1). The intestinal segments are mounted in Ussing chambers or are alternatively used as everted intestinal sacs or rings (2, 3). The available in vitro models have made it possible to perform relatively detailed studies on intestinal drug transport. However, these models have a number of limitations. Firstly, they are not of human origin. In addition, they are often difficult to perform and only a limited number of experiments can be performed on each occasion. The viability of isolated intestinal segments is also limited which at some occasions reduces the duration of the experiments to only a few minutes (3). However, experiments that extend over a few hours can usually be performed in Ussing chambers.

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References

  1. Jackson, M.J. in Physiology of the Gastrointestinal Tract, Second Edition (Johnson, L.R. Ed.), Raven Press, New York 1987, chapter 59, p. 1597–1621.

    Google Scholar 

  2. Ussing, H.H. and Zehran, K., Acta Physiol. Scand. 23, 110–127 (1951).

    Article  PubMed  CAS  Google Scholar 

  3. Gardner, C.R. in Directed Drug Delivery (Borchardt, R.T., Tepta, A.J. and Stella, V.J. Eds.), Humana Press Inc., Clifton, NJ, 1985, p. 61–82.

    Google Scholar 

  4. Pinto, M. et al., Biol. Cell 47, 323–330 (1983).

    Google Scholar 

  5. Grasset, E. et al., Am. J. Physiol. 247, C260–C267 (1984).

    PubMed  CAS  Google Scholar 

  6. Chantret, L et al., Cancer Res. 48, 1936–1942 (1988).

    PubMed  CAS  Google Scholar 

  7. Neutra, M. and Louvard, D. in Modern Cell Biology, vol. 8: Functional Epithelial Cells in Culture (Matlin, K.S. and Valentich, J.D. Eds.), Alan R. Liss, New York, 1989, p. 363–398.

    Google Scholar 

  8. Hidalgo, I.J., Raub, T.J. and Borchardt, R.T., Gastroenterology 96, 736–749 (1989).

    PubMed  CAS  Google Scholar 

  9. Wilson, G. et al., J. Controlled Release 11, 25–40 (1990).

    Article  CAS  Google Scholar 

  10. Artursson, P., J. Pharm. Sci. 79(5) 1990, in press.

    Google Scholar 

  11. Fogh, J., Fogh, J.M. and Orfeo, T., J. Natl. Cancer Inst. 59, 221–226 (1977).

    PubMed  CAS  Google Scholar 

  12. Hidalgo, I.J. and Borchardt. R.T., Pharm. Res. 5 (suppl.), S–110 (PD949) (1988).

    Google Scholar 

  13. Hidalgo, I.J. and Borchardt. R.T., Pharm. Res. 5 (suppl.), S–110 (PD950) (1988).

    Google Scholar 

  14. Muthia, R. and Seetharam, B., J. Cell Biol. 105, 235a (1987).

    Article  Google Scholar 

  15. Dix, C.J. et al., Biochem. Soc. Trans. 15, 439–440 (1987).

    CAS  Google Scholar 

  16. Grasset, E., Bernabeu, J. and Pinto, M., Am. J. Physiol. 248, C410–C418 (1985).

    PubMed  CAS  Google Scholar 

  17. Mohrmann, I. et al., Am. J. Physiol. 250, G323–G330 (1986).

    PubMed  CAS  Google Scholar 

  18. Burnham, D.B. and Fondacaro, J.D., Am. J. Physiol. 256, G808–G816 (1989).

    PubMed  CAS  Google Scholar 

  19. Hauri, H.P. et al., J. Cell Biol. 101, 838–851 (1985).

    Article  PubMed  CAS  Google Scholar 

  20. Artursson, P. and Magnusson, C., J. Pharm. Sci. 79(6) 1990, in press.

    Google Scholar 

  21. Karlsson, J. and Artursson, P., Proc. Int. Union Physiol. Sci. XVII, P1306 (1989).

    Google Scholar 

  22. Madara, J.L. and Dharmsathaporn, K., J. Cell Biol. 101, 2124–2133 (1985).

    Article  PubMed  CAS  Google Scholar 

  23. Madara, J.L. and Trier, J.S. in Physiology of the Gastrointestinal Tract, Second Edition (Johnson, L.R. Ed.), Raven Press, New York 1987, chapter 44, p. 1209–1249.

    Google Scholar 

  24. Pappenheimer, J.R. and Reiss, K.Z., J. Membr. Biol. 100, 123–136 (1987).

    Article  PubMed  CAS  Google Scholar 

  25. Pappenheimer, J.R., J. Membr. Biol. 100, 137–148 (1987).

    Article  PubMed  CAS  Google Scholar 

  26. Lowe, PJ. et al., Am. J. Physiol. 255, G454–G461 (1988).

    PubMed  CAS  Google Scholar 

  27. Madara, J.L. and Stafford, J., J. Clin. Invest. 83, 724–727 (1989).

    Article  PubMed  CAS  Google Scholar 

  28. Powell, D.W. in Physiology of the Gastrointestinal Tract, Second Edition (Johnson, L.R. Ed.), Raven Press, New York 1987, chapter 46, p. 1267–1305.

    Google Scholar 

  29. Ungell, A.-L. et al., Proc. 3rd Int. Conf. Drug Absorption, Edinburgh, 27–30 September 1988, p. 34.

    Google Scholar 

  30. Taylor, D.C., Pownall, R. and Burke, W., J. Pharm. Pharmacol. 37, 280–283 (1985).

    Article  PubMed  CAS  Google Scholar 

  31. Schoenwald, R.D. and Huang, H.-S., J. Pharm. Sci. 72, 1266–1271 (1983).

    Article  PubMed  CAS  Google Scholar 

  32. Wilson, F.A. and Dietschy, J.M., Biochem. Biophys. Acta 363, 112–126 (1974).

    Article  PubMed  CAS  Google Scholar 

  33. Anderson, B.W. et al., Am. J. Physiol. 254, G843–G848 (1988).

    PubMed  CAS  Google Scholar 

  34. Komiya, I. et al., Int. J. Pharm. 4, 249–262 (1980).

    Article  CAS  Google Scholar 

  35. Gumbier, B., Stvenson, B. and Grimaldi, A., J. Cell Biol. 107, 1575–1587 (1988).

    Article  Google Scholar 

  36. Martinez-Palomo, A. et al., J. Cell Biol. 87, 736–745 (1980).

    Article  PubMed  CAS  Google Scholar 

  37. Sedar, A.W. and Forte, J.G., J. Cell Biol. 22, 173–188 (1964).

    Article  PubMed  CAS  Google Scholar 

  38. Cereijido, M. et al., 77, 853-880 (1978).

    Google Scholar 

  39. Burnette, R.R. in Controlled Drug Delivery, Fundamentals and Applications 2nd Ed. (Robinson, J.R. and Lee, V.H. Eds.) Marcel Dekker, Inc., New York, 1987, p. 95–138.

    Google Scholar 

  40. Ho, N.F.H. et al. in Animal Models for Oral Drug Delivery in Man: In Situ and In Vivo Approaches (Crouthamel, W. and Sarapu, A.C. Eds.) American Pharmaceutical Association, Washington DC, USA, 1983, p. 27–107.

    Google Scholar 

  41. Cerda, J.J. et al., J. Parenter. Enterai Nutr. 11, 63–66 (1987).

    Article  CAS  Google Scholar 

  42. Gerencser, G.A. et al., Proc. Soc. Exp. Biol. Med. 176, 183–186 (1984).

    PubMed  CAS  Google Scholar 

  43. Westergaard, H. and K.H. and Dietschy, J.M., J. Clin. Invest. 54, 718–732 (1974).

    Article  PubMed  CAS  Google Scholar 

  44. Westergaard, H., Holtermuller, K.H. and Dietschy, J.M., Am. J. Physiol. 250, G727–G735 (1986).

    PubMed  CAS  Google Scholar 

  45. Flourie, B. et al., Gut. 25, 936–941 (1984).

    Article  PubMed  CAS  Google Scholar 

  46. Frase. L.L. et al., Gastroenterology 88, 478–484 (1985).

    PubMed  CAS  Google Scholar 

  47. Levitt, M.D. et al., J. Clin. Invest. 74, 2056–2064 (1984).

    Article  PubMed  CAS  Google Scholar 

  48. Levitt, M.D. et al., Am. J. Physiol. 252, G325–G332 (1987).

    PubMed  CAS  Google Scholar 

  49. Read, N.W. et al., Gut. 18, 865–876 (1977).

    Article  PubMed  CAS  Google Scholar 

  50. Sparso, B.H., Luke, M. and Wium, E., Scand. J. Gastroenterol. 19, 568–574 (1984).

    PubMed  CAS  Google Scholar 

  51. Barry, P.H. and Diamond, J.M., Physiol. Rev. 64, 763–872 (1984).

    PubMed  CAS  Google Scholar 

  52. Pedley, T.J., Q. Rev. Biophys. 16, 115–150 (1983).

    Article  PubMed  CAS  Google Scholar 

  53. Lynch, J. et al., Proc. 3rd Int. Conf. Drug Absorption, Edinburgh, 27–30 September, 1988, p. 34.

    Google Scholar 

  54. Johno, I. et al., Int. J. Pharm. 25, 255–263 (1985).

    Article  CAS  Google Scholar 

  55. Madara, J.L., J. Cell Biol. 97, 125–136 (1983).

    Article  PubMed  CAS  Google Scholar 

  56. Paulmichl, M. et al., Pflügers Arch. 413, 456–462 (1989).

    Article  PubMed  CAS  Google Scholar 

  57. Griepp, E.B. et al., J. Cell Biol. 96, 693–702 (1983).

    Article  PubMed  CAS  Google Scholar 

  58. Kiviluoto, T., Mustonen, H. and Kivilaakso, E., Gastroenterology 96, 1410–1418 (1989).

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pharmaceutics, Uppsala University, Uppsala, Sweden

    Per Artursson & Johan Karlsson

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  1. Per Artursson
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  2. Johan Karlsson
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Editors and Affiliations

  1. SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania, USA

    Glynn Wilson

  2. University of Nottingham, Nottingham, UK

    S. S. Davis  & L. Illum  & 

  3. INSERM, Paris, France

    Alain Zweibaum

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© 1991 Plenum Press, New York

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Artursson, P., Karlsson, J. (1991). Passive Absorption of Drugs in Caco-2 Cells. In: Wilson, G., Davis, S.S., Illum, L., Zweibaum, A. (eds) Pharmaceutical Applications of Cell and Tissue Culture to Drug Transport. NATO ASI Series, vol 218. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0286-6_8

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  • DOI: https://doi.org/10.1007/978-1-4757-0286-6_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0288-0

  • Online ISBN: 978-1-4757-0286-6

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