Carcinogen Metabolism in Immortalised Human Cells Grown as Hybrid Cells in Culture
Most cells from normal tissues have two characteristic properties. Their cell division is regulated in a particular way and they produce substances characteristic only of their tissue of origin. It was soon realised, however, that as cells from normal tissues were studied extensively, a limit to their long term cultivation was found. Normal cells died after a finite number of divisions. In contrast, tumour cells grew indefinitely in culture and usually did not express differentiated functions. In order to distinguish between these types of cells with finite or infinite lifespan in culture, Hayflick and Moorhead (1) used the term cell strain to denote normal cells with a finite lifespan and reserved the term cell line for cells which were established in culture and would divide indefinitely. They also noted that the property of infinite cell growth was usually associated with a change in the diploid nature of the cells and that a heteroploid karyotype was common in permanent cell lines.
KeywordsHybrid Cell Epoxide Hydrolase Aryl Hydrocarbon Hydroxylase Permanent Cell Line Aryl Hydrocarbon Hydroxylase Activity
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