Human Nasal Epithelial Cultures

  • Richard C. Boucher
  • Elaine H. C. Cheng
Part of the NATO ASI Series book series (NSSA, volume 218)


The proximal airway epithelia act in a coordinated manner to defend the lung from the effects of inhaled foreign materials. The defense mechanisms provided by these airway epithelia are usually analyzed within the framework of the mucociliary clearance system. Nasal epithelia are similar to those of the proximal intrapulmonary airways as both surfaces are populated by relatively equal numbers of ciliated, secretory, and basal cells. The motive force required to move materials trapped in airway surface liquids (ASL) to the oropharynx is provided by the cilia of the ciliated cell.


Cystic Fibrosis Cholera Toxin Airway Epithelium Tissue Culture Plastic Nasal Epithelium 
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  1. 1.
    Knowles, M.R., M.J. Stutts, J.R. Yankaskas, J.T. Gatzy and R.C. Boucher. Abnormal respiratory epithelial ion transport in cystic fibrosis. (Review) Clin. Chest Med. 7: 285–297 (1986).PubMedGoogle Scholar
  2. 2.
    Finney, M.J.B., S.D. Anderson, J.L. Black. The effect of non-isotonic solutions on human isolated airway smooth muscle. Respir. Physiol. 69: 277–286 (1987).PubMedCrossRefGoogle Scholar
  3. 3.
    Wu, R., J. Yankaskas, E. Cheng, M.R. Knowles and R. Boucher. Growth and differentiation of human nasal epithelial cells in culture: serum-free, hormone-supplemented medium and proteoglycan synthesis. Am. Rev. Respir. Dis. 132: 311–320 (1985).PubMedGoogle Scholar
  4. 4.
    Van Scott, Michael R., N.P. Lee, J.R. Yankaskas, and R.C. Boucher. Effect of hormones on growth and function of cultured canine tracheal epithelial cells. Am. J. Phvsiol. 255 (Cell Phvsiol. 24); C237–245 (1988).Google Scholar
  5. 5.
    Yankaskas, J.R., C.U. Cotton, M.R. Knowles, J.T. Gatzy, and R.C. Boucher. Culture of human nasal epithelial cells on collagen matrix supports. Am. Rev. Respir. Dis. 132; 1281–1287 (1985).PubMedGoogle Scholar
  6. 6.
    Burg, M., N. Green, S. Sohraby, R. Steele, J. Handler. Differentiated function in cultured epithelia derived from thick ascending limbs. Am. J. Physiol. 242: 229–233 (1982).Google Scholar
  7. 7.
    Yankaskas, J.R., J.T. Gatzy and R.C. Boucher. Persistence of abnormal Cl permeability in cystic fibrosis nasal epithelial cells in heterologous culture. Lancet 1: 954–956 (1985).PubMedCrossRefGoogle Scholar
  8. 8.
    Boucher, R.C, C.U. Cotton, J.T. Gatzy, M.R. Knowles, and J.R. Yankaskas. Evidence for reduced Cl and increased Na+ permeability in cystic fibrosis human primary cell cultures. J. Phvsiol. 405: 77–103, 1988.Google Scholar
  9. 9.
    Willumsen, Niels J., C. William Davis, and Richard C. Boucher. Intracellular Cl activity and Cl pathways in cultured human airway epithelium. Am. J. Physiol. 256 (Cell Phvsiol. 25): C1033–C1044 (1989).PubMedGoogle Scholar
  10. 10.
    Boucher, Richard C., Elaine H.C. Cheng, Anthony M. Paradiso, M. Jackson Stutts, Michael R. Knowles, and H. Shelton Earp. The chloride secretory response of cystic fibrosis (CF) human airway epithelia: Preservation of calcium but not protein kinase C and A — Dependent mechanisms. (In Press J. Clin. Invest., 1989).Google Scholar
  11. 11.
    Cheng, Pi-Wan, Thomas F. Boat, Karen Cranfill, James R. Yankaskas, and Richard C. Boucher. Increased sulfation of glycoconjugates by cultured nasal epithelial cells from patients with cystic fibrosis. J. Clin. Invest. 84: 68–72 (1989).PubMedCrossRefGoogle Scholar
  12. 12.
    Henke, David, Roberta M. Danilowicz, John F. Curtis, Richard C. Boucher and Thomas E. Eling. Metabolism of arachidonic acid by human nasal and bronchial epithelial cells. Arch. of Biochem. and Biophysics 267: 426–436 (1988).CrossRefGoogle Scholar
  13. 13.
    Yoakum, George H., John F. Lechner, Edward W. Gabrielson, Brent E. Korba, Louise Malan-Shibley, James C. Wiley, Marion G. Valerio, Abulkalam M. Shamsuddin, Benjamin F. Trump, Curtis C. Harris. Transformation of human bronchial epithelial cells transfected by harvey ras oncogene. Science 227: 1174–1179 (1985).PubMedCrossRefGoogle Scholar
  14. 14.
    Jetten, Anton M., James R. Yankaskas, M. Jackson Stutts, Niels J. Willumsen, and Richard C. Boucher. Persistence of abnormal chloride conductance regulation in transformed cystic fibrosis epithelia. Science 244: 1472–1475 (1989).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Richard C. Boucher
    • 1
  • Elaine H. C. Cheng
    • 1
  1. 1.School of MedicineUniversity of North CarolinaChapel HillUSA

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