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Growth and Characterization of Cell and Tissue Cultures for the Study of Drug Transport

  • Glynn Wilson
Part of the NATO ASI Series book series (NSSA, volume 218)

Abstract

Recent advances in cell and tissue culture methodologies, particularly the growth of differentiated human cells, are providing new and potentially powerful tools for cellular and molecular biology studies on the processes involved in differentiation and in the intracellular sorting of molecules and membranes. These techniques are also beginning to be used for investigating the transport of drug molecules and delivery systems into specific cells and across specific biological barriers. A particular need is to have in vitro systems that can be employed to devise new strategies for the absorption and delivery of new drug classes e.g. peptides and proteins. Cell and tissue culture systems provide the potential for rapidly evaluating the permeability and metabolism of a drug, for defining the mechanisms of transport of drugs and delivery systems, and for testing novel strategies for enhancing drug transport and drug targeting. In addition they may provide the opportunity to use human rather than animal tissues. Cell and tissue culture systems, that display many of the morphological and functional properties of in vivo cell layers, have been established. These include epithelial barriers that form the intestinal, rectal, buccal, sublingual and nasal mucosae; cells that form the epidermis of the skin, and vascular endothelial barriers e.g. brain capillary endothelial cells. This review will discuss factors that influence the growth and characterization of in vitro systems as it pertains to their use in studies on the transport of drug molecules. Intestinal epithelial systems will be used to illustrate specific points.

Keywords

Cell Culture System Microporous Membrane Ussing Chamber Tissue Culture System Taurocholic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Glynn Wilson
    • 1
  1. 1.Department of Drug DeliverySmithKline Beecham PharmaceuticalsKing of PrussiaUSA

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