Cultured Human Umbilical Vascular Endothelial Cells and Their Underlying Matrix as Models to Study Cell Adhesion

  • Eva Bastida
Part of the NATO ASI Series book series (NSSA, volume 218)


Vascular endothelium is the blood compatible container that covers blood vessels. The endothelial cell layer lining of the circulatory system, although structurally simple, is functionally complex and its integrity is essential for normal vascular function1. Although tissue culture techniques for mammalian cells were established about two decades ago, the specific requirements of vascular endothelial cells hampered their routine culture until rather recently. Jaffe and coworkers were the first who succeeded in growing vascular endothelial cells in the laboratory2. The source of cells were veins from human umbilical cords. The cells were isolated by incubation with the enzyme collagenase and cultured on glass or plastic surfaces in the presence of specific culture media supplemented with growth factors. More recently, other types of vessels have been used to obtain vascular endothelial cells, including bovine and swine aorta and human adult saphenous vein. Moreover, microcapillary endothelium has also been isolated and cultured. This represents an interesting advance that allows the study of the differences in the structure and properties of endothelial cells from large and small vessels.


Vascular Endothelial Cell Human Umbilical Cord Endothelial Cell Culture Tissue Culture Technique Human Umbilical Vascular Endothelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    J.J. Sixma, “Role of blood vessel, platelet, and coagulation interactions.” In: Haemostasis and Thrombosis A. L Bloom and D.P. Thomas (Eds.). Churchill Livingstone: Edinburgh 1982; 252–267.Google Scholar
  2. 2.
    E.A. Jaffe, R.L. Nachmann, C.G. Becker, “Culture of human endothelial cells derived from umbilical veins identified by morphometrical and immunological criteria.” J. Clin. Invest. 1973; 52: 2745–2752.PubMedCrossRefGoogle Scholar
  3. 3.
    M.A. Gimbrone, Jr., “Culture of vascular endothelium.” Prog. Hemost. Thromb. 1976, 3: 1–28.PubMedGoogle Scholar
  4. 4.
    P.G. De Groot, J.H. Reinders, J.J. Sixma, “Perturbation of human endothelial cells by thrombin or PMA changes the reactivity of their extracellular matrix towards platelets.” J. Cell Biol. 1987; 104: 697–704.PubMedCrossRefGoogle Scholar
  5. 5.
    J. Aznar-Salatti, E. Bastida, G. Escolar, P. De Groot, M.R. Buchanan, A. Ordinas, “The method of exposure of endothelial cell extracellular matrix influences its thrombogeneicity.” Atherosclerosis 1989 (In Press).Google Scholar
  6. 6.
    M.R. Buchanan, E. Bastida, “The role of 13-HODE and HETEs in vessel wall/circulating cell interactions.” Agents and Actions 1987; 22: 1–4.CrossRefGoogle Scholar
  7. 7.
    G.L. Nicolson, “Metastatic tumor cell attachment and invasion assay using vascular endothelial cell monolayers”. J. Histochem Cytochem 1982; 30: 214–220.PubMedCrossRefGoogle Scholar
  8. 8.
    E. Bastida, L. Almirall, A. Ordinas, “Platelet and shear rate promote tumor cell adhesion to human endothelial extracellular matrix — Absence of a role for platelet cyclooxygenase.” Thrombosis Haemost 1989; 61: 485–489.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Eva Bastida
    • 1
  1. 1.Cell Biology LaboratoryR&D Laboratorios MenariniBarcelonaSpain

Personalised recommendations