Regulation of the Myocardial Blood Supply by Adenosine and Adenine Nucleotides: Consequences of Newly Detected Barrier Functions of the Coronary Endothelium

  • S. Nees
  • A. Dendorfer
Part of the NATO ASI Series book series (NSSA, volume 218)


In the classical physiological view coronary flow is regulated mainly by certain metabolic products that are liberated by the cardiomyocytes, in amounts that increase with increasing cardiac work-load. Like chemical transmitters, these products are thought to develop their dilatory action directly at smooth muscle cells of the coronary resistance vessels. Adenosine, which is mainly derived by stepwise dephosphorylation of the central energy carrier of the myocardial metabolism, 5′-ATP, is still considered to be the most important mediator substance of this metabolic regulation of coronary flow (1) (fig 1).


Coronary Flow Adenine Nucleotide Percoll Gradient Coronary System Coronary Endothelial Cell 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R.M. Berne, The role of adenosine in the regulation of coronary blood flow, Circ. Res. 47: 807–813 (1980).PubMedCrossRefGoogle Scholar
  2. 2.
    S. Nees, V. Herzog, B.F. Becker, M. Böck, C. des Rosiers, and E. Gerlach, The coronary endothelium: a highly active metabolic barrier for adenosine, Bas. Res. Cardiol. 80: 515–529 (1985).CrossRefGoogle Scholar
  3. 3.
    S. Nees, The adenosine hypothesis of metabolic regulation of coronary flow in the light of newly recognized properties of the coronary endothelium, Z. Kardiol., in press (1989).Google Scholar
  4. 4.
    M. Bundgaard, P. Hagman, C. Crone, The three-dimensional organization of plasmalemmal vesicular profiles in the endothelium of rat heart capillaries, Microvasc. Res. 25: 358–368 (1983).PubMedCrossRefGoogle Scholar
  5. 5.
    P.M. Vanhoutte, G.M. Rubanyi, V.M. Miller, and D.S. Houston, Modulation of vascular smooth muscle contraction by the endothelium, Ann. Rev. Physiol. 48: 307–320 (1986).CrossRefGoogle Scholar
  6. 6.
    M.J. Lew, and B.R. Duling, Differential arteriolar responses to luminally and extraluminally applied agonists and antagonists: evidence for an endothelial cell diffusion barrier, FASEB-Journal 2: A944 (abstr. 3774) (1988). Paper in press, Am. J. Physiol. (1989).Google Scholar
  7. 7.
    S. Nees, Coronary flow increases induced by adenosine and adenine nucleotides are mediated by the coronary endothelium: a new concept of the metabolic regulation of coronary flow, Europ. J. Cardiol., in press (1989).Google Scholar
  8. 8.
    B.F. Becker, and E. Gerlach, Uric acid, the major catabolite of cardiac adenine nucleotides and adenosine, originates in the coronary endothelium, in: “Topics and Perspectives in Adenosine Research”, E. Gerlach and B.F. Becker, eds., Springer, Berlin, Heidelberg, New York, pp. 209–222 (1987).CrossRefGoogle Scholar
  9. 9.
    C. des Rosiers, S. Nees, E. Gerlach, Purine metabolism in cultured aortic and coronary endothelial cells, Biochem. Cell Biol. 67: 8–15 (1989).PubMedCrossRefGoogle Scholar
  10. 10.
    C. des Rosiers, and S. Nees, Functional evidence for the presence of adenosine A2-receptors in cultured coronary endothelial cells, Naunyn-Schmiedeberg’s Arch. Pharmacol. 336: 94–98 (1987).Google Scholar
  11. 11.
    W.H. Newman, B.F. Becker, M. Heier, S. Nees, and E. Gerlach, Endothelium-mediated coronary dilation by adenosine does not depend on endothelial adenylate cyclase activation: studies on isolated guinea pig hearts. Pflüqers Arch. 413: 1–7 (1988).CrossRefGoogle Scholar
  12. 12.
    S. Nees, C. des Rosiers, and M. Böck, Adenosine receptors at the coronary endothelium: functional implications, in: “Topics and Perspectives in Adenosine Research”, E. Gerlach and B.F. Becker, eds., Springer, Berlin, Heidelberg, New York, pp. 454–469 (1987).CrossRefGoogle Scholar
  13. 13.
    P. Anversa, V. Levicky, C. Beghi, S.L. McDonald, and Y. Kikkana, Morphometry of exercise-induced right ventricular hypertrophy in the rat, Circ. Res. 52: 57–64 (1983).PubMedCrossRefGoogle Scholar
  14. 14.
    J.B. Bassingthwaighte, T. Yipintsoi, and R.B. Harvey, Microvasculature of the dog left ventricular myocardium, Microvasc. Res. 7: 229–249 (1974).PubMedCrossRefGoogle Scholar
  15. 15.
    J. Daut, G. Mehrke, S. Nees, and W.H. Newman, Passive electrical properties and electrogenic sodium transport of cultured guinea-pig coronary endothelial cells, J. Physiol. 402: 237–254 (1988).PubMedGoogle Scholar
  16. 16.
    O. Hudlická, Growth of capillaries in skeletal and cardiac muscle. Circ. Res. 50: 451–461 (1982).PubMedCrossRefGoogle Scholar
  17. 17.
    P.A. Netland, B.R. Zetter, D.P. Via, J.C. Voyta, In situ labelling of vascular endothelium with fluorescent acetylated low density lipoprotein, Histochem. J. 17: 1309–1320 (1985).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • S. Nees
    • 1
  • A. Dendorfer
    • 1
  1. 1.Dept. of PhysiologyUniversity of MunichMunich 2Germany

Personalised recommendations