Metabolism of Catecholamine Prodrugs by Cultured Bovine Brain Microvessel Endothelial Cells

  • Gerhard K. E. Scriba
  • Ronald T. Borchardt
Part of the NATO ASI Series book series (NSSA, volume 218)


The endothelium of the brain capillaries, the anatomical site of the blood-brain barrier, consists of a single layer of cells. In contrast to the peripheral microvasculature, it is characterized by tight intracellular junctions, the absence of fenestrations and few pinocytotic vesicles (Brightman, 1977). Moreover, high enzymatic activity including metabolizing enzymes such as monoamine oxidase (MAO; EC (Lai et al., 1975; Hardebo et al., 1980), cholinesterases (Joo and Csillik, 1966; Duricic and Mrsulja, 1977), aminopeptidases (Brecher et al., 1978) and cytochrome P-450 (Ghersi-Egea et al., 1988) have been reported in the microvessel wall. Both, anatomical and enzymatic properties of the endothelium, are responsible for the restricted movement of solutes from the blood into the brain.


Endothelial Cell Monolayer Microvessel Endothelial Cell Diisopropyl Fluorophosphate DOPAC Level Brain Microvessel Endothelial Cell 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Gerhard K. E. Scriba
    • 1
  • Ronald T. Borchardt
    • 2
  1. 1.School of PharmacyUniversity of MünsterMünsterGermany
  2. 2.School of PharmacyUniversity of KansasLawrenceUSA

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