Culture of Human Goblet Cells

  • Christian L. Laboisse
Part of the NATO ASI Series book series (NSSA, volume 218)


Mucins comprise a heterogeneous set of high molecular weight glycoproteins (HMGs). All mucins share several characteristics: they are rich in serine and threonine and contain several hundreds of oligosaccharide chains in O-glycosidic linkages to these aminoacids. The gel-forming properties of mucins are essential for the protection of the epithelial lining of the gastrointestinal tract The mucus gel was first thought of as being a physical barrier whose main function was to shield against harmful agents. As a consequence, the lesions of the gastrointestinal mucosa were assumed to be largely due to qualitative or quantitative defects of this gel. Now several recent studies suggest that intestinal mucins should be viewed as an essential component of a complex ecosystem which comprises in the distal part of the intestine, the colonic microflora, the luminal content, the epithelial lining of the mucosa and its secretory products.


Goblet Cell Vasoactive Intestinal Peptide Colonic Epithelial Cell Mucus Secretion Colonic Crypt 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    M. Deschuyteneer, A.E. Eckhardt, J. Roth, and R.L. Hill, The subcellular localization of apomucin and nonreducing terminal N-Acetylgalactosamine in porcine submaxillary glands, J. Biol. Chem. 263: 2452 (1988).PubMedGoogle Scholar
  2. 2.
    H. Schachter, and D. Williams, Biosynthesis of mucus glycoproteins, in: “Mucus in Health and Disease”, Part II, Advances in experimental Medicine and Biology vol. 144, E.N. Chantier, J.B. Elder, and M. Elstein eds., Plenum Press, New York (1982).Google Scholar
  3. 3.
    DJ. Taatjes, J. Roth, J. Weinstein, and J.C. Paulson, Post-golgi apparatus localization and regional expression of rat intestinal sialyltransferase detected by immunoelectron microscopy with polypeptide epitope-purified antibody. J. Biol. Chem. 263: 6302 (1988).PubMedGoogle Scholar
  4. 4.
    M.R. Neutra, and J.F. Forstner, Gastrointestinal mucus: synthesis, secretion, and function, in: “Physiology of the gastrointestinal tract” L.R. Johnson, J. Christensen, M.J. Jackson, E.D. Jacobson, and J.H. Walsh eds., Raven Press, New York (1987).Google Scholar
  5. 5.
    J. Bara, F. Loisillier, and P. Burtin, Antigens of gastric and intestinal mucous cells in human colonic tumours. Br. J. Cancer 41: 209 (1980).PubMedCrossRefGoogle Scholar
  6. 6.
    L. Chambraud, A. Bernadac, J.P. Gorvel, and S. Maroux, Renewal of goblet cell mucus granules during the cell migration along the crypt-villus axis in rabbit jejunum: an immunolabeling study, Biol. Cell 65: 151 (1989).PubMedCrossRefGoogle Scholar
  7. 7.
    N. Roomi, M. Laburthe, N. Fleming, R. Crowther, and J. Forstner, Cholera-induced mucin secretion of rat intestine: lack of effect of cAMP, cycloheximide, VIP, and colchicine, Am. J. Physiol. (Gastrointest liver physiol. 10) 247: G140 (1984).PubMedGoogle Scholar
  8. 8.
    J.S. Trier, Organ-culture methods in the study of gastrointestinal-mucosal function and development, New Engl. J. Med. 295: 150 (1976).PubMedCrossRefGoogle Scholar
  9. 9.
    J.T. LaMont, and A.S. Ventola, Synthesis and secretion of colonic glycoproteins, Biochim. Biophvs. Acta. 629: 553 (1980).CrossRefGoogle Scholar
  10. 10.
    M.R. Neutra, R.J. Grand, and J.S. Trier, Glycoprotein synthesis, transport, and secretion by epithelial cells of human rectal mucosa, Lab. Invest. 26: 535 (1977).Google Scholar
  11. 11.
    A.C. Smith, and D.K. Podolsky, Biosynthesis and secretion of human colonic mucin glycoproteins, J. Clin. Invest. 80: 300 (1987).PubMedCrossRefGoogle Scholar
  12. 12.
    R.D. Specian, and M.R. Neutra, Regulation of intestinal mucus secretion.I. Role of parasympathetic stimulation. Am. J. Phvsiol. 242 (Gastrointest. Liver Physiol. 5): G370 (1982).Google Scholar
  13. 13.
    M.R. Neutra, L.J. O’Malley, and R.D. Specian, Regulation of intestinal goblet cell secretion.II. A survey of potential secretagogues. Am. J. Physiol. 242 (Gastrointest. Liver Physiol. 5): G380 (1982).PubMedGoogle Scholar
  14. 14.
    T.A. Brasitus, Isolation of proliferative epithelial cells from the rat cecum and proximal colon. Anal. Biochem. 123: 364 (1982).PubMedCrossRefGoogle Scholar
  15. 15.
    DJ. Ahnen, T.A. Reed, and J.M. Bozdech, Isolation and characterization of populations of mature and immature rat colonocytes, Am. J. Phvsiol. 254 (Gastrointest. Liver Physiol. 17): G610 (1988).Google Scholar
  16. 16.
    J.D. Kaunitz, Preparation and characterization of viable epithelial cells from rabbit distal colon, Am. J. Phvsiol. 254 (Gastrointest. Liver Physiol. 17): G502 (1988).Google Scholar
  17. 17.
    M. Buset, S. Winawer, and E. Friedman, Defining conditions to promote the attachment of adult human colonic epithelial cells, In Vitro Cell. Devel. Biol. 23: 403 (1987).CrossRefGoogle Scholar
  18. 18.
    D.P. Chopra, K.M. Siddiqui, and R.A. Cooney, Effects of insulin, transferrin, cholera toxin, and epidermal growth factor on growth and morphology of human fetal normal colon epithelial cells, Gastroenterology 92: 891 (1987).PubMedGoogle Scholar
  19. 19.
    R.H. Whitehead, A. Brown, and P.S. Bhathal, A method for the isolation and culture of human colonic crypts in collagen gels, in Vitro Cell. Devel. Biol. 23: 436 (1987).CrossRefGoogle Scholar
  20. 20.
    P.R. Gibson, E. van de Pol, L.E. Maxwell, A. Gabriel, and W.F. Doe, Isolation of colonic crypts that maintain structural and metabolic viability in vitro, Gastroenterology. 96: 283 (1989).PubMedGoogle Scholar
  21. 21.
    C. Paraskeva, B.G. Buckle, D. Sheer, and C.B. Wigley, The isolation and characterization of colorectal epithelial cell lines at different stages in malignant transformation from familial polyposis coli patients, Int. J. Cancer, 34: 49 (1984).PubMedCrossRefGoogle Scholar
  22. 22.
    J.K.V. Willson, G.N. Bittner, T.D. Oberley, L.F. Meisner, and J.L. Weese, Cell culture of human colon adenomas and carcinomas, Cancer Res. 47: 2704 (1987).PubMedGoogle Scholar
  23. 23.
    C. Paraskeva, S. Finerty, and S. Powell, Immortalization of a human colorectal adenoma cell line by continuous “in vitro” passage: possible involvement of chromosome 1 in tumour progression, Int. J. Cancer 41: 908 (1988).PubMedCrossRefGoogle Scholar
  24. 24.
    M.P. Moyer, and J.B. Aust, Human colon cells: culture and in vitro transformation, Science 224: 1445 (1984).PubMedCrossRefGoogle Scholar
  25. 25.
    R.D. Berry, S.C. Powell, and C. Paraskeva, “In vitro” culture of human foetal colonic epithelial cells and their transformation with origin minus SV40 DNA, Br. J. Cancer 57: 287 (1988).PubMedCrossRefGoogle Scholar
  26. 26.
    S. Emami, L. Mir, C. Gespach, and G. Rosselin, Transfection of fetal rat intestinal epithelial cells by viral oncogenes: establishment and characterization of the ElA-immortalized SLC-11 cell lines. Proc. Natl. Acad. Sci. USA 86: 3194 (1989).PubMedCrossRefGoogle Scholar
  27. 27.
    C.L. Laboisse, Differentiation of colon cells in culture, in: “Cell and molecular biology of colon cancer”, L.H. Augenlicht, ed., CRC Press, Boca Raton (1989).Google Scholar
  28. 28.
    C.L. Laboisse, The structure of gastrointestinal mucins: searching for the Rosetta stone, Biochimie 68: 611 (1986).PubMedCrossRefGoogle Scholar
  29. 29.
    C. Augeron, and C.L. Laboisse, Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate, Cancer Res. 44: 3961 (1984).PubMedGoogle Scholar
  30. 30.
    J. Fogh, and G. Trempe, New human tumor cell lines, in “Human Tumor Cells “in vitro””, J. Fogh, ed., Plenum Press, New York, (1975).Google Scholar
  31. 31.
    C. Augeron, J.J. Maoret, C.L. Laboisse, and E. Grasset, Permanently differentiated cell clones isolated from the human colonic adenocarcinoma cell line HT29: possible models for the study of ion transport and mucus production, in “Ion gradient-coupled transport”, F. Alvarado and C.H. Van Os, eds., INSERM symposium N°26, Elsevier (1986).Google Scholar
  32. 32.
    J.J. Maoret, J. Font, C. Augeron, P. Codogno, C. Bauvy, M. Aubery, and C.L. Laboisse, A mucussecreting human colonic cancer cell line — purification and partial characterization of the secreted mucins, Biochem. J. 258: 793 (1989).PubMedGoogle Scholar
  33. 33.
    A.M. Roberton, M. Mantle, R.E.F. Fahim, R.D. Specian, A. Bennick, S. Kawagishi, P. Sherman, and J.F. Forstner, The putative “link” glycopeptide associated with mucus glycoproteins — composition and properties of preparations from the gastrointestinal tracts of several mammals, Biochem. J. 261: 637 (1989).PubMedGoogle Scholar
  34. 34.
    I. Roumagnac, and C. Laboisse, A mucus-secreting human colonic epithelial cell line responsive to cholinergic stimulation, Biol. Cell 61: 65 (1987).PubMedCrossRefGoogle Scholar
  35. 35.
    I. Roumagnac, and C.L. Laboisse, A simple immunofiltration assay for mucins secreted by a human colonic epithelial cell line, J. Immunol, Methods 122: 265 (1989).CrossRefGoogle Scholar
  36. 36.
    M. Laburthe, C. Augeron, C. Rouyer-Fessard, I. Roumagnac, J.J. Maoret, E. Grasset, and C. Laboisse, Functional VIP receptors in the human mucus-secreting colonic epithelial cell line Cl.16E, Am. J. Phvsiol. 256 (Gastrointest. Liver Physiol. 19): G443 (1989).Google Scholar
  37. 37.
    C.L. Laboisse, J.J. Maoret, N. Triadou, and C. Augeron, Restoration by polyethylene glycol of characteristics of intestinal differentiation in subpopulations of the human colonic adenocarcinoma cell Une HT29. Cancer Res. 48: 2498 (1988).PubMedGoogle Scholar
  38. 38.
    M. Kedinger, K. Haffen, and P. Simon-Assmann, Intestinal tissue and cell cultures, Differentiation 36: 71 (1987).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Christian L. Laboisse
    • 1
  1. 1.Faculté BichatU239 INSERMParisFrance

Personalised recommendations