Culture of Human Goblet Cells
Mucins comprise a heterogeneous set of high molecular weight glycoproteins (HMGs). All mucins share several characteristics: they are rich in serine and threonine and contain several hundreds of oligosaccharide chains in O-glycosidic linkages to these aminoacids. The gel-forming properties of mucins are essential for the protection of the epithelial lining of the gastrointestinal tract The mucus gel was first thought of as being a physical barrier whose main function was to shield against harmful agents. As a consequence, the lesions of the gastrointestinal mucosa were assumed to be largely due to qualitative or quantitative defects of this gel. Now several recent studies suggest that intestinal mucins should be viewed as an essential component of a complex ecosystem which comprises in the distal part of the intestine, the colonic microflora, the luminal content, the epithelial lining of the mucosa and its secretory products.
KeywordsGoblet Cell Vasoactive Intestinal Peptide Colonic Epithelial Cell Mucus Secretion Colonic Crypt
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