An in vitro Absorption Model System Based on Cell Monolayers (Intestine 407, MDBK)
Human in vivo studies on the physicochemical properties of drugs are difficult to perform, first of all because of ethic reasons. For similar reasons the number of animal tests should be reduced if possible. Therefore alternative methods are desired such as biological in vitro experiments based on cell cultures. Thus, the development of a suitable in vitro absorption model system based on defined cell monolayers may offer some advantages if the experiments are performed in very controlled conditions. This is an important precondition, because in vivo as well as in vitro biological experiments usually exhibit important variances in their results, i.e. relative standard errors around 30–40% are rather common. Many papers even present results without an estimate of the experimental error involved. With the in vitro absorption model system presented here, two goals can be pursued simultaneously: a possible reduction in the number of animal tests, such as e.g. in situ rat intestine perfusion experiments and hopefully a possible reduction of experimental error.
KeywordsSalicylic Acid Cell Monolayer Permeability Coefficient Octanoic Acid Lipophilic Drug
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