Abstract
Somaclonal variation is defined as genetic variation observed among progeny of plants regenerated from somatic cells cultured in vitro. Although theoretically all plants regenerated from somatic cells should be clones, a number of observations have indicated that this is not the case.1,2 In addition to the basic genetic implications of this phenomenon, the variation has proven useful in breeding programs of various crop plants.3 Conventional plant breeding has traditionally been the sole avenue for crop improvement; however, recent demands of agricultural-based industry has shown that this process is too time consuming for typical corporate time frames. While variability has been obtained from wild species of cultivated crops its access has been quite limited. Indeed many desired traits such as disease resistance have yet to be identified. It is also difficult to establish novelty related to legal protection of conventional breeding outputs since many workers are using the same breeding lines in their programs. Thus somaclonal variation provides great promise for reducing the time required to produce new varieties or breeding lines which are easily patentable due to their novel variation.
Keywords
- Breeding Line
- Somatic Hybrid
- Somaclonal Variation
- Crop Improvement
- Plant Cell Culture
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, access via your institution.
Buying options
Preview
Unable to display preview. Download preview PDF.
References
Larkin, P.J., W.R. Scowcroft. 1981. Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197–214.
Evans, D.A., W.R. Sharp, H.P. Medina-Filho. 1984. Somaclonal and gametoclonal variation. Amer. J. Bot. 71: 759–774.
Evans, D.A., W.R. Sharp. 1986. Somaclonal and gametoclonal variation. In Handbook of Plant Cell Culture. (D.A. Evans et al., eds.), Vol. 4, Macmillan Publishing Company, New York, pp. 97–132.
Scowcroft, W.R. 1985. Somaclonal variation: the myth of clonal uniformity. In Genetic Flux in Plant. (B. Hahn, E.S. Dennis, eds.), Springer Verlag, New York, pp. 112–156.
D’amato, F. 1952. Polyploidy in the differentiation and function of tissues and cells in plants. A critical examination of the literature. Caryologia 4: 311–357.
Evans, D.A., J.E. Bravo. 1986. Phenotypic and genotypic stability of tissue cultured plants. In Tissue Culture as a Plant Production System for Horticultural Crops. (R.H. Zimmerman et al., eds.), Martinus Nijhoff Publishers, Dordrecht, The Netherlands, pp. 73–94.
George, L., P.S. Rao. 1983. Yellow seeded variants in in vitro regenerants of mustard (Brassica juncea cross var. RA1-5). Plant Sci. Lett. 30: 327–330.
Evans, D.A., W.R. Sharp. 1983. Single gene mutations in tomato plants regenerated from tissue culture. Science 221: 949–951.
Groose, R.W., E.T. Bingham. 1984. Variation in plants regenerated from tissue culture of tetraploid alfalfa heterozygous for several traits. Crop Sci. 24: 655–658.
Dulieu, H., M. Barbier. 1982. High frequencies of genetic variant plants regenerated from cotyledons of tobacco, In Variability in Plants Regenerated from Tissue Culture. (L. Earle, Y. Demarly, eds.), Praegar Press, New York, pp. 211–299.
Thanutong, P., I. Furusawa, M. Yammato. 1983. Resistant tobacco plants from protoplast-derived calluses selected for their resistance to Pseudomonas and Alternaria toxins. Theor. Appl. Genet. 66: 209–215.
Landsmann, J., H. Uhrig. 1985. Somaclonal variation in Solanum tuberosum detected at the molecular level. Theor. Appl. Genet. 71: 500–505.
Brettell, R.I.S., E.S. Dennis, W.R. Scowcroft, W.J. Peacock. 1986. Molecular analysis of a somaclonal variant of maize alcohol dehydrogenase. Mol. Gen. Genet. 202: 235–239.
Chaleff, R.S. 1981. Genetics of higher plants. Applications of cell culture. Cambridge University Press, New York.
Meins, F. 1983. Heritable variation in plant cell culture. Ann. Rev. Plant Physiol. 34: 327–346.
Chourey, P.S., R.J. Kemble. 1982. Transposition event in tissue cultured cells of S-cms genotype of maize. Maize Genet. Coop. Newslett. 56: 70.
Lorz, H., W.R, Scowcroft. 1983. Variability among Plants and their progeny regenerated from protoplasts of Su/su heterozygotes of Nicotiana tabacum. Theor. Appl. Genet. 66: 67–75.
Evans, D.A., J.E. Bravo, S.A. Kut, C.E. Flick. 1983. Genetic behavior of somatic hybrids in the genus Nicotiana: N. otophora + N. tabacum and N. sylvestris + N. tabacum. Theor. Appl. Genet. 65: 93–101.
Kemble, R.J., R.I.S. Brettell, R.B. Flavell. 1982. Mitochondrial DNA analyses of fertile and sterile maize plants from tissue culture with the Texas male sterile cytoplasm. Theor. Appl. Genet. 62: 213–217.
Kemble, R.J., J.F. Shepard. 1984. Cytoplasmic DNA variation in a potato protoclonal population. Theor. Appl. Genet. 69: 211–216.
Bendich, A.J., L.P. Gauriloff. 1984. Morphometric analysis of cucurbit mitochondria: the relationship between chondriome volume and DNA content. Protoplasma 119: 1–7.
Sanders, M.E., C.J. Franzke. 1976. Effect of temperature on origin of colchicine-induced complex mutants in sorghum. J. Hered. 67: 19–29.
Morrison, R.A., D.A. Evans. 1987. Gametoclonal variation. In Plant Breeding Reviews. (J. Janick, ed.), Vol. 5, AVI Publishing, Westport, Connecticut, pp. 359–391.
Morrison, R.A. 1986. Gametoclonal Variation in Pepper. Ph.D. Dissertation, Rutgers — The State University of New Jersey, Piscataway, New Jersey.
Flick, C.E. 1984. Isolation of mutants from cell cultures. In D.A. Evans et al., eds., op. cit. Reference 3, Vol. 1, pp. 393–441.
Chaleff, R.S. 1983. Isolation of agronomically useful mutants from plant cell cultures. Science 219: 676–682.
Ling, D.H., P. Vidhyaseharan, E.S. Borromeo, F.J. Zapata, T.W. Mew. 1985. In vitro screening of rice germplasm for resistance to brown spot disease using phytotoxin. Theor. Appl. Genet. 71: 133–135.
Shahin, E.A., R. Spivey. 1986. A single dominant gene for Fusarium wilt resistance in protoplast-derived tomato plants. Theor. Appl. Genet. 73: 164–169.
Peerbolte, R., P. Ruigrok, G. Wullems, R. Schilperoort. 1987. T-DNA rearrangements due to tissue culture: somaclonal variation in crown gall tissues. Plant Molec. Biol. 9: 51–57.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Plenum Press, New York
About this chapter
Cite this chapter
Morrison, R.A., Whitaker, R.J., Evans, D.A. (1988). Somaclonal Variation: Its Genetic Basis and Prospects for Crop Improvement. In: Conn, E.E. (eds) Opportunities for Phytochemistry in Plant Biotechnology. Recent Advances in Phytochemistry, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0274-3_1
Download citation
DOI: https://doi.org/10.1007/978-1-4757-0274-3_1
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-0276-7
Online ISBN: 978-1-4757-0274-3
eBook Packages: Springer Book Archive