Isolation of Cell Surface Membrane Fractions from Mammalian Cells and Organs

  • David M. NevilleJr.


The use of cell surface membrane fractions in biochemical research has changed from a rarity to a commonplace over the last 5 yr. The interest in these fractions stems from the important functions of the surface membrane in the living cell. The cell surface membrane is the physical boundary which separates the internal environment of the cell from the external environment, and all interactions between the cell and its environment must take place across this boundary. All free energy gradients which exist at this boundary—chemical, electrostatic, or mechanical—are due in large part to the properties of the membrane. The ways in which the membrane handles these gradients may be thought of as specialized functions of the membrane. These physiological functions consist of information transfer to and from the environment and membrane-mediated responses to the environments. The latter include transport of substances entering and leaving the cell, modification of substances at the cell surface, and the mechanical processes of cell locomotion and division. One can see that much of biology and biochemistry occurs at this interface. Consequently, the number of investigators wishing to use cell surface membrane fractions is large and their backgrounds are diverse.


Surface Membrane Membrane Fraction Brush Border Microsomal Fraction Morphological Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allan, D., and Crumpton, M. J., 1970, Preparation and characterization of the plasma membrane of pig lymphocytes, Biochem. J. 120:133–143.PubMedGoogle Scholar
  2. Allan, D., and Crumpton, M. J., 1972, Isolation and composition of human thymocyte plasma membrane, Biochim. Biophys. Acta 274:22–27.PubMedCrossRefGoogle Scholar
  3. Amir, S. M., Carraway, T. F., Jr., Kohn, L. D., and Winand, R. J., 1973, The binding of thyrotropin to isolated bovine thyroid plasma membranes, J. Biol. Chem. 248: 4092–4100.PubMedGoogle Scholar
  4. Anderson, N. G., Harris, W. W., Barber, A. A., Rankin, C., Jr., and Candler, E. L., 1966, Separation of subcellular components and viruses by combined rate-and isopycnic-zonal centrifugation, Natl. Cancer. Inst. Monogr. 21:253–283.PubMedGoogle Scholar
  5. Andrew, C. G., and Appel, S. H., 1973, Macromolecular characterization of muscle membranes. I. Proteins and sialic acid of normal and denervated muscle, J. Biol. Chem. 248:5156–5163.PubMedGoogle Scholar
  6. Atkinson, P. H., and Summers, D. F., 1971, Purification and properties of HeLa cell plasma membranes, J. Biol. Chem. 246:5162–5175.PubMedGoogle Scholar
  7. Avruch, J., and Wallach, D. F. H., 1971, Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells, Biochim. Biophys. Acta 233:334–347.PubMedCrossRefGoogle Scholar
  8. Berger, S. J., and Sacktor, B., 1970, Isolation and biochemical characterization of brush borders from rabbit kidney, J. Cell Biol. 47:637–645.PubMedCrossRefGoogle Scholar
  9. Bergeron, J. J. M., Evans, W. H., and Geschwind, I.I., 1973, Insulin binding to rat liver Golgi fractions, J. Cell Biol. 59:771–776.PubMedCrossRefGoogle Scholar
  10. Berman, H. M., Gram, W., and Spirtes, M. A., 1969, An improved, reproducible method of preparing rat liver plasma cell membranes in buffered isotonic sucrose, Biochim. Biophys. Acta 183:10–18.PubMedCrossRefGoogle Scholar
  11. Bingham, R. W., and Burke, D. C., 1972, Isolation of plasma membrane and endoplasmic reticulum fragments from chick embryo fibroblasts, Biochim. Biophys. Acta 274:348–352.PubMedCrossRefGoogle Scholar
  12. Binkley, F., King, N., Milikin, E., Wright, R. K., O’Neal, C. H., and Wundram, I. J., 1968, Brush border particulates of renal tissue, Science 162:1009–1011.PubMedCrossRefGoogle Scholar
  13. Boone, C. W., Ford, L. E., Bond, H. E., Stuart, D. C., and Lorenz, D., 1969, Isolation of plasma membrane fragments from HeLa cells, J. Cell Biol. 41:378–392.PubMedCrossRefGoogle Scholar
  14. Bosmann, H. B., Hagopian, A., and Eylar, E. H., 1968, Cellular membranes: The isolation and characterization of the plasma and smooth membranes of HeLa cells, Arch. Biochem. Biophys. 128:51–69.PubMedCrossRefGoogle Scholar
  15. Brunette, D. M., and Till, J. E., 1971, A rapid method for the isolation of L-cell surface membranes using an aqueous two-phase polymer system, J. Membrane Biol. 5: 215–224.CrossRefGoogle Scholar
  16. Carroll, P. M., and Sereda, D. D., 1968, Cell membrane of uterine smooth muscle, Nature (Lond.) 217:666–667.CrossRefGoogle Scholar
  17. Charalampous, F. C., Gonatas, N. K., and Melbourne, A. D., 1973, Isolation and properties of the plasma membrane of KB cells, J. Cell Biol. 59:421–435.PubMedCrossRefGoogle Scholar
  18. Coleman, R., Michell, R. H., Finean, J. B., and Hawthorne, J. N., 1967, A purified plasma membrane fraction isolated from rat liver under isotonic conditions, Biochim. Biophys. Acta 135:573–579.PubMedCrossRefGoogle Scholar
  19. Cotman, C. W., and Matthews, D. A., 1971, Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterization, Biochim. Biophys. Acta 249: 380–394.PubMedCrossRefGoogle Scholar
  20. Cotman, C. W., McCaman, R. E., and Dewhurst, S. A., 1971, Subsynaptosomal distribution of enzymes involved in the metabolism of lipids, Biochim. Biophys. Acta 249: 395–405.PubMedCrossRefGoogle Scholar
  21. de Duve, C., 1971, Tissue fractionation, past and present, J. Cell Biol. 50:20D–55D.PubMedCrossRefGoogle Scholar
  22. de Duve, C., Pressman, B. C., Gianetto, R., Wattiaux, R., and Appelmans, F., 1955, Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue, Biochem. J. 60:604–617.Google Scholar
  23. Demus, H., 1973, Subcellular fractionation of human lymphocytes: Isolation of two plasma membrane fractions and comparison of the protein components of the various lymphocytic organelles, Biochim. Biophys. Acta 291:93–106.PubMedCrossRefGoogle Scholar
  24. DePierre, J. W., and Karnovsky, M. L., 1973, Plasma membranes of mammalian cells: A review of methods for their characterization and isolation, J. Cell Biol. 56:275–303.PubMedCrossRefGoogle Scholar
  25. DeRobertis, E., Azcurra, J. M., and Fiszer, S., 1966, Ultrastructure and cholinergic binding capacity of junctional complexes isolated from rat brain. Brain Res. 5:45–56.CrossRefGoogle Scholar
  26. Devynck, M.-A., Pernollet, M.-G., Meyer, P., Fermandjian, S., and Fromageot, P., 1973, Angiotensin receptors in smooth muscle cell membranes, Nature New Biol. 245:55–57.PubMedGoogle Scholar
  27. Dods, R. F., Essner, E., and Barclay, M., 1972, Isolation and characterization of plasma membranes from an L-asparaginase-sensitive strain of leukemia cells, Biochem. Biophys. Res. Commun. 46:1074–1081.PubMedCrossRefGoogle Scholar
  28. Dorling, P. R., and LePage, R. N., 1973, A rapid high yield method for the preparation of rat liver cell plasma membranes, Biochim. Biophys. Acta 318:33–40.PubMedCrossRefGoogle Scholar
  29. Douglas, A. P., Kerley, R., and Isselbacher, K. J., 1972, Preparation and characterization of the lateral and basal plasma membranes of the rat intestinal epithelial cell, Biochem. J. 128:1329–1338.PubMedGoogle Scholar
  30. El-Aaser, A. A., Fitzsimons, J. T. R., Hinton, R. H., Reid, E., Klucis, E., and Alexander, P., 1966, Zonal centrifugation of crude nuclear fractions from rat liver, Biochim. Biophys. Acta 127:553–556.PubMedCrossRefGoogle Scholar
  31. Emmelot, P., and Bos, C. J., 1966, Differences in the association of two glycolytic enzymes with plasma membranes isolated from rat liver and hepatoma, Biochim. Biophys. Acta 121:434–436.PubMedCrossRefGoogle Scholar
  32. Emmelot, P., and Bos, C. J., 1969a, Studies on plasma membranes. IX. A survey of enzyme activities displayed by plasma membranes isolated from normal and preneoplastic livers and primary and transplanted hematomas of the rat, Int. J. Cancer 4:705–722.PubMedCrossRefGoogle Scholar
  33. Emmelot, P., and Bos, C. J., 1969b, Studies on plasma membranes. X. A survey of enzyme activities displayed by plasma membranes isolated from mouse liver and three mouse hematoma strains, Int. J. Cancer 4:723–734.PubMedCrossRefGoogle Scholar
  34. Emmelot, P. C., Bos, C. J., Benedetti, E. L., and Rümke, P., 1964, Studies on plasma membranes. I. Chemical composition and enzyme content of plasma membranes isolated from rat liver, Biochim. Biophys. Acta 90:126–145.PubMedCrossRefGoogle Scholar
  35. Evans, W. H., 1970, Fractionation of liver plasma membranes prepared by zonal centrifugation, Biochem. J. 116:833–842.PubMedGoogle Scholar
  36. Farquhar, M. G., Bergeron, J. J. M., and Palade, G. E., 1974, Cytochemistry of Golgi fractions prepared from rat liver, J. Cell Biol. 60:8–25.PubMedCrossRefGoogle Scholar
  37. Finn, F. M., Widnell, C. C., and Hofmann, K., 1972, Localization of an adreno-corticotropic hormone receptor on bovine adrenal cortical membranes, J. Biol. Chem. 247:5695–5702.PubMedGoogle Scholar
  38. Fitzpatrick, D. F., Davenport, G. R., Forte, L., and Landon, E. J., 1969, Characterization of plasma membrane proteins. I. Preparation of a membrane fraction and separation of the protein, J. Biol. Chem. 244:3561–3569.PubMedGoogle Scholar
  39. Fleischer, B., and Fleischer, S., 1969, Glycosidase activity of bovine liver plasma membranes, Biochim. Biophys. Acta 183:265–275.PubMedCrossRefGoogle Scholar
  40. Forstner, G. G., Sabesin, S. M., and Isselbacher, K. J., 1968, Rat intestinal microvillus membranes: Purification and biochemical characterization, Biochem. J. 106:381–390.PubMedGoogle Scholar
  41. Forte, J. G., Forte, T. ML., and Heinz, E., 1973, Isolation of plasma membranes from Ehrlich ascites tumor cells. Influence of amino acids on (Na+ + K+)-ATPase and K+-stimulated phosphatase, Biochim. Biophys. Acta 298:827–841.PubMedCrossRefGoogle Scholar
  42. Freychet, P., Roth, J., and Neville, D. M., Jr., 1971, Insulin receptors in the liver: Specific binding of [125I] insulin to the plasma membrane and its relation to insulin bio-activity, Proc. Natl. Acad. Sci. 68:1833–1837.PubMedCrossRefGoogle Scholar
  43. Fujita, M., Ohta, H., Kawai, K., Matsui, H., and Nakao, M., 1972, Differential isolation of microvillous and basolateral plasma membranes from intestinal mucosa: Mutually exclusive distribution of digestive enzymes and ouabain-sensitive ATPase, Biochim. Biophys. Acta 274:336–347.PubMedCrossRefGoogle Scholar
  44. Giacobino, J. P., and Perrelet, A., 1971, Preparation of a plasma membrane fraction from the brown adipose tissue, Experentia 27:259–261.CrossRefGoogle Scholar
  45. Glaumann, H., and Daliner, G., 1970, Subfractionation of smooth microsomes from rat liver, J. Cell Biol. 47:34–48.PubMedCrossRefGoogle Scholar
  46. Glossmann, H., and Neville, D. M., Jr., 1972, Phlorizin receptors in isolated kidney brush border membranes, J. Biol. Chem. 247:7779–7789.PubMedGoogle Scholar
  47. Goldfine, I. D., Kahn, C. R., Neville, D. M., Jr., Roth, J., Garrison, M. M., and Bates, R. W., 1973, Decreased binding of insulin to its receptors in rats with hormone induced insulin resistance, Biochem. Biophys. Res. Commun. 53:852–857.PubMedCrossRefGoogle Scholar
  48. Gospodarowicz, D., 1973, Preparation and characterization of plasma membranes from bovine corpus luteum, J. Biol. Chem. 248:5050–5056.PubMedGoogle Scholar
  49. Gregoriadis, G., Morell, A. G., Sternlieb, I., and Scheinberg, I. H., 1970, Catabolism of desialylated ceruloplasmin in the liver, J. Biol. Chem. 245:5833–5837.PubMedGoogle Scholar
  50. Heidrich, H.-G., Kinne, R., Kinne-Saffran, E., and Hannig, K., 1972, The polarity of the proximal tubule cell in rat kidney: Different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings, J. Cell Biol. 54:232–245.PubMedCrossRefGoogle Scholar
  51. Hemminki, K., and Suovaniemi, O., 1973, Preparation of plasma membranes from isolated cells of newborn rat brain, Biochim. Biophys. Acta 298:75–83.PubMedCrossRefGoogle Scholar
  52. Henn, F. A., Hansson, H.-A., and Hamberger, A., 1972, Preparation of plasma membrane from isolated neurons, J. Cell Biol. 53:654–661.PubMedCrossRefGoogle Scholar
  53. Henning, R., Kaulen, H. D., and Stoffel, W., 1970, Biochemical analysis of the pinocytotic process. I. Isolation and chemical composition of the lysosomal and the plasma membrane of the rat liver cell, Hoppe-Seylers Z. Physiol. Chem. 351:1191–1199.PubMedCrossRefGoogle Scholar
  54. Herzenberg, L. A., and Herzenberg, L. A., 1961, Association of H-2 antigens with the cell membrane fraction of mouse liver, Proc. Natl. Acad. Sci. 47:762–767.PubMedCrossRefGoogle Scholar
  55. Hicks, R. M., and Ketterer, B., 1970, Isolation of the plasma membrane of the luminal surface of rat bladder epithelium, and the occurrence of a hexagonal lattice of subunits both in negatively stained whole mounts and in sectioned membranes, J. Cell Biol. 45:542–553.PubMedCrossRefGoogle Scholar
  56. Hinton, R. H., 1972, Purification of plasma-membrane fragments, in: Subcellular Components (G. D. Birnie, ed.), pp. 119–155, Butterworths, London, University Park Press, Baltimore.Google Scholar
  57. Hinton, R. H., Dobrota, M., Fitzsimons, J. T. R., and Reid, E., 1970, Preparation of a plasma membrane fraction from rat liver by zonal centrifugation, Eur. J. Biochem. 12:349–361.PubMedCrossRefGoogle Scholar
  58. House, P. D. R., and Weidman, M. J., 1970, Characterization of an [125I]-insulin binding plasma membrane fraction from rat liver, Biochem. Biophys. Res. Commun. 41: 541–548.PubMedCrossRefGoogle Scholar
  59. Israel, A., Verjus, M.-A., and Semmel, M., 1973, Isolation and characterization of surface membranes from chorioallantoic cells and chick fibroblasts, Biochim. Biophys. Acta 318:155–166.PubMedCrossRefGoogle Scholar
  60. Kahn, C. R., Neville, D. M., Jr., and Roth, J., 1973, Insulin-receptor interaction in the obese-hyperglycemic mouse, J. Biol. Chem. 248:244–250.PubMedGoogle Scholar
  61. Kamat, V. B., and Wallach, D. F. H., 1965, Separation and partial purification of plasmamembrane fragments from Ehrlich ascites carcinoma microsomes, Science 148: 1343–1345.PubMedCrossRefGoogle Scholar
  62. Kasper, C. B., 1971, Biochemical distinctions between the nuclear and microsomal membranes from rat hepatocytes: The effect of phenobarbital administration, J. Biol. Chem. 246:577–581.PubMedGoogle Scholar
  63. Keeran, T. W., Morré, D. J., Olson, D. E., Yunghans, W. N., and Patton, S., 1970, Biochemical and morphological comparison of plasma membrane and milk fat globule membrane from bovine mammary gland, J. Cell Biol. 44:80–93.CrossRefGoogle Scholar
  64. Kennel, S. J., and Lerner, R. A., 1973, Isolation and characterization of plasma membrane associated immunoglobulin from cultured human diploid lymphocytes, J. Mol. Biol. 76:485–502.PubMedCrossRefGoogle Scholar
  65. Kidwai, A. M., Radcliffe, M. A., and Daniel, E. E., 1971a, Studies on smooth muscle plasma membrane. I. Isolation and characterization of plasma membrane from rat myometrium, Biochim. Biophys. Acta 233:538–549.CrossRefGoogle Scholar
  66. Kidwai, A. M., Radcliffe, M. A., Duchon, G., and Daniel, E. E., 1971b, Isolation of plasma membrane from cardiac muscle, Biochem. Biophys. Res. Commun. 45:901–910.PubMedCrossRefGoogle Scholar
  67. Kinne, R., and Kinne-Saffran, E., 1969, Isolierung und enzymatische Charakterisierung einer Bürstensaumfraktion du Rattenniere, Pfluegers Arch. Eur. J. 308:1–15.CrossRefGoogle Scholar
  68. Klenk, H.-D., and Choppin, P. W., 1969, Lipids of plasma membranes of monkey and hamster kidney cells and of parainfluenza virions grown in these cells, Virology 38:255–268.PubMedCrossRefGoogle Scholar
  69. Kono, T., and Colowick, S. P., 1961, Isolation of skeletal muscle cell membrane and some of its properties, Arch. Biochem. Biophys. 93:520–533.PubMedCrossRefGoogle Scholar
  70. Korn, E. D., 1974a, Biochemistry of the mammalian plasma membrane, in: The Inflammatory Process, Vol. 1, 2nd ed. (B. F. Zweifach, L. Grant, and R. T. McCluskey, eds.), pp. 52–113, Academic Press, New York.Google Scholar
  71. Korn, E. D., 1974b, The isolation of the amoeba plasma membrane and the use of latex beads for the isolation of phagocytic vesicle membranes from amoebae, in: Methods in Enzymology, Vol. 31 Part A (S. Fleischer, ed.), pp. 686–698, Academic Press, New York.Google Scholar
  72. Korn, E. D., 1974c, Biochemistry of endocytosis, in: Biochemistry of Cell Walls and Membranes (C. F. Fox, ed.), pp. 1–26, Medical and Technical Publishing Co., London.Google Scholar
  73. Koyama, N., Sawada, K., and Kurihra, K., 1971, Isolation and some properties of plasma membranes from bovine olfactory epithelium, Biochim. Biophys. Acta 241:42–48.CrossRefGoogle Scholar
  74. Križanová, O., and Čiampor, F., 1971, Interaction of plasma membranes with influenza virus. I. Isolation of plasma membranes from chick embryo cells, Acta Virol. 15: 19–27.PubMedGoogle Scholar
  75. Laudat, M. H., Pairault, J., Bayer, P., Martin, M., and Laudat, P., 1972, Preparation of fat cell membrane with high sensitivity to lipolytic hormones, Biochim. Biophys. Acta 255:1005–1008.PubMedCrossRefGoogle Scholar
  76. Lauter, C. J., Solyom, A., and Trams, E. G., 1972, Comparative studies on enzyme markers of liver plasma membranes, Biochim. Biophys. Acta 266:511–523.PubMedCrossRefGoogle Scholar
  77. Lentens, L. V., and Ashwell, G., 1971, Studies on the chemical and enzymatic modification of glycoproteins, J. Biol. Chem. 246:1889–1894.Google Scholar
  78. Lesko, L., Donlon, M., Marinetti, G. V., and Hare, J. D., 1973, A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system, Biochim. Biophys. Acta 311:173–179.PubMedCrossRefGoogle Scholar
  79. Levitan, I. B., Mushynski, W. E., and Ramirez, G., 1972, Highly purified synaptosomal membranes from rat brain: Preparation and characterization, J. Biol. Chem. 247: 5376–5381.PubMedGoogle Scholar
  80. Lo, C.-H., 1973, The plasma membranes of bovine circumvallate papillae: Isolation and partial characterization, Biochim. Biophys. Acta 291:650–661.PubMedCrossRefGoogle Scholar
  81. Lutz, F., and Frimmer, M., 1970, A new method for the isolation of plasma membranes from pig liver, Hoppe-Seylers Z. Physiol. Chem. 351:1429–1434.PubMedCrossRefGoogle Scholar
  82. Marx, S. J., Fedak, S. A., and Aurbach, G. D., 1973a, Preparation and characterization of a hormone-responsive renal plasma membrane fraction, J. Biol. Chem. 247: 6913–6918.Google Scholar
  83. Marx, S. J., Woodward, C., Aurbach, G. D., Glossmann, H., and Keutmann, H. T., 1973b, Renal receptors for calcitonin: Binding and degradation of hormone, J. Biol. Chem. 248:4797–4802.PubMedGoogle Scholar
  84. McCollester, D. L., 1962, A method for isolating skeletal-muscle cell-membrane components, Biochim. Biophys. Acta 57:427–437.CrossRefGoogle Scholar
  85. McKeel, D. W., and Jarett, L., 1970, Preparation and characterization of a plasma membrane fraction from isolated fat cells, J. Cell Biol. 44:417–432.PubMedCrossRefGoogle Scholar
  86. McKeel, D. W., Jr., and Jarett, L., 1974, The enrichment of adenylate cyclase in the plasma membrane and Golgi subcellular fractions of porcine adenohypophysis, J. Cell Biol. 62:231–236.PubMedCrossRefGoogle Scholar
  87. Meldolesi, J., Jamieson, J. D., and Palade, G. E., 1971, Composition of cellular membranes in the pancreas of the guinea pig. III. Enzymatic activities, J. Cell Biol. 49:150–158.PubMedCrossRefGoogle Scholar
  88. Miller, D., and Crane, R. K., 1961, The digestive function of the epithelium of the small intestine. I. An intracellular locus of disaccharide and sugar phosphate ester hydrolysis, Biochim. Biophys. Acta 52:281–293.PubMedCrossRefGoogle Scholar
  89. Molnar, J., Markovic, G., and Chao, H., 1969, Glycoproteins of Ehrlich ascites carcinoma cells: Separation of plasma and endoplasmic reticular membrane fragments, Arch. Biochem. Biophys. 134:524–532.PubMedCrossRefGoogle Scholar
  90. Morgan, I. G., Wolfe, L. S., Mandel, P., and Gambos, G., 1971, Isolation of plasma membranes from rat brain, Biochim. Biophys. Acta 241:737–751.PubMedCrossRefGoogle Scholar
  91. Neville, D. M., Jr., 1960, The isolation of a cell membrane fraction from rat liver, J. Biophys. Biochem. Cytol. 8:413–422.PubMedCrossRefGoogle Scholar
  92. Neville, D. M., Jr., 1968, Isolation of an organ specific protein antigen from cell surface membrane of rat liver, Biochim. Biophys. Acta 154:540–552.PubMedGoogle Scholar
  93. Neville, D. M., Jr., 1974, Receptors for polypeptide hormones: Direct studies of insulin binding to purified liver plasma membranes, In Vitro, 9:445–454.Google Scholar
  94. Neville, D. M., Jr., and Kahn, C. R., 1974, Isolation of plasma membranes for cell surface membrane receptor studies, in: Methods in Molecular Biology, Vol. 5: Subcellular Particles, Structures and Organelles (A. I. Laskin and J. A. Last, eds.), pp. 57–88, Dekker, New York.Google Scholar
  95. Neville, D. M., Jr., Kahn, C. R., Soll, A., and Roth, J., 1973, Plasma membrane insulin receptor defect in obese mice, in: Protides of the Biological Fluids (XXI Colloquium, May 1973, Brugge, H. Peeters, ed.), pp. 269–273, Pergamon Press.Google Scholar
  96. Nigam, V. N., Morais, R., and Karasaki, S., 1971, A simple method for the isolation of rat liver cell plasma membranes in isotonic sucrose, Biochim. Biophys. Acta 249: 34–40.PubMedCrossRefGoogle Scholar
  97. Perdue, J. F., and Sneider, J., 1970, The isolation and characterization of the plasma membrane from chick embryo fibroblasts, Biochim. Biophys. Acta 196:125–140.PubMedCrossRefGoogle Scholar
  98. Perdue, J. F., Kletzien, R., and Miller, K., 1971a, The isolation and characterization of plasma membrane from cultured cells. I. The chemical composition of membrane isolated from uninfected and oncogenic RNA virus-converted chick embryo fibroblasts, Biochim. Biophys. Acta 249:419–434.PubMedCrossRefGoogle Scholar
  99. Perdue, J. F., Kletzien, R., Miller, K., Pridmore, G., and Wray, V. L., 1971b, The isolation and characterization of plasma membranes from cultured cells. II. The chemical composition of membrane isolated from uninfected and oncogenic RNA virus-converted parenchyma-like cells, Biochim. Biophys. Acta 249:435–453.PubMedCrossRefGoogle Scholar
  100. Perdue, J. F., Warner, D., and Miller, K., 1973, The isolation and characterization of plasma membrane from cultured cells. V. The chemical composition of plasma membranes isolated from chicken tumors initiated with virus-transformed cells, Biochim. Biophys. Acta 298:817–826.PubMedCrossRefGoogle Scholar
  101. Peters, T. J., Müller, M., and de Duve, C., 1972, Lysosomes of the arterial wall. I. Isolation and subcellular fractionation of cells from normal rabbit aorta, J. Exp. Med. 136: 1117–1139.PubMedCrossRefGoogle Scholar
  102. Pfleger, R. C., Anderson, N. G., and Snyder, F., 1968, Lipid class and fatty acid composition of rat liver plasma membranes isolated by zonal centrifugation, Biochemistry 7:2826–2833.PubMedCrossRefGoogle Scholar
  103. Pohl, S. L., Birnbaumer, L., and Rodbell, M., 1971, The glucagon-sensitive adenyl cyclase system in plasma membranes of rat liver. I. Properties, J. Biol. Chem. 246:1849–1856.PubMedGoogle Scholar
  104. Poirier, G., Labrie, F., Barden, N., and Lemaire, S., 1972, Thyrotropin-releasing hormone receptor: Its partial purification from bovine anterior pituitary gland and its close association with adenyl cyclase, FEBS Letters 20:283–286.PubMedCrossRefGoogle Scholar
  105. Poirier, G., DeLean, A., Pelletier, G., Lemay, A., and Labrie, F., 1974, Purification of adenohypophyseal plasma membranes and properties of associated adenylate cyclase, J. Biol. Chem. 249:316–322.PubMedGoogle Scholar
  106. Porteous, J. W., 1972, The isolation of brush borders (microvilli) from the epithelial cells of mammalial intestine, in: Subcellular Components (G. D. Birnie, ed.), pp. 157–183, Butterworths, London, University Park Press, Baltimore.Google Scholar
  107. Pricer, W. E., and Ashwell, G., 1971, The binding of desialylated glycoproteins by plasma membranes of rat liver, J. Biol. Chem. 246:4825–4833.PubMedGoogle Scholar
  108. Raftell, M., and Blomberg, F., 1973, Membrane fractions from rat hepatoma. I. Isolation and characterization, Biochim. Biophys. Acta 291:421–430.PubMedCrossRefGoogle Scholar
  109. Ragland, W. L., Pace, J. L., and Doak, R. L., 1973, Plasma membranes of bursal, thymic and circulating lymphocytes have unique proteins, Biochem. Biophys. Res. Commun. 50:118–126.PubMedCrossRefGoogle Scholar
  110. Ray, T. K., 1970, A modified method for the isolation of the plasma membrane from rat liver, Biochim. Biophys. Acta 196:1–9.PubMedCrossRefGoogle Scholar
  111. Renkonen, O., Gahmberg, C. G., Simons, K., and Kääriäinen, L., 1970, Enrichment of gangliosides in plasma membranes of hamster kidney fibroblasts, Acta Chem. Scand. 24:733–735.PubMedCrossRefGoogle Scholar
  112. Reporter, M., and Raveed, D., 1973, Plasma membranes: Isolation from naturally fused and lysolecithin-treated muscle cells, Science 181:863–865.PubMedCrossRefGoogle Scholar
  113. Rodbell, M., 1967, Metabolism of isolated fat cells. V. Preparation of “ghosts” and their properties; adenyl cyclase and other enzymes, J. Biol. Chem. 242:5744–5750.PubMedGoogle Scholar
  114. Rosenberg, M. D., 1969, Plasma membranes of liver cells of the chick embryo. I. Isolation procedures, Biochim. Biophys. Acta 173:11–22.PubMedCrossRefGoogle Scholar
  115. Rosenthal, S. L., Edelman, P. M., and Schwartz, I. L., 1965, A new method for the preparation of skeletal muscle sarcolemma, Biochim. Biophys. Acta 109:512–517.PubMedCrossRefGoogle Scholar
  116. Rothschild, J., 1963, The isolation of microsomal membranes, Biochem. Soc. Symp. 22:4–31.PubMedGoogle Scholar
  117. Russell, T., and Pastan, I., 1973, Plasma membrane cyclic adenosine 3′,5′-monophosphate phosphodiesterase of cultured cells and its modification after trypsin treatment of intact cells, J. Biol. Chem. 248:5835–5840.PubMedGoogle Scholar
  118. Ryan, J. W., and Smith, U., 1971, A rapid, simple method for isolating pinocytotic vesicles and plasma membrane of lung, Biochim. Biophys. Acta 249:177–180.PubMedCrossRefGoogle Scholar
  119. Silverman, M., Aganon, M. A., and Chinard, F. P., 1970, D-Glucose interactions with renal tubule cell surfaces, Am. J. Physiol. 218:735–742.PubMedGoogle Scholar
  120. Solyom, A., and Trams, E. G., 1972, Enzyme markers in characterization of isolated plasma membranes, Enzyme 13:329–372.PubMedGoogle Scholar
  121. Song, C. S., Rubin, W., Rifkind, A. B., and Kappas, A., 1969, Plasma membranes of the rat liver: Isolation and enzymatic characterization of a fraction rich in bile canaliculi, J. Cell Biol. 41:124–132.PubMedCrossRefGoogle Scholar
  122. Steck, T. L., 1972, Membrane isolation, in: Membrane Molecular Biology (C. F. Fox and A. D. Keith, eds.), pp. 76–114, Sinauer Associates, Stamford, Conn.Google Scholar
  123. Stein, Y., Widnell, C., and Stein, O., 1968, Acylation of lysophosphatides by plasma membrane fractions of rat liver, J. Cell Biol. 39:185–192.PubMedCrossRefGoogle Scholar
  124. Stonehill, E. H., and Huppert, J., 1968, Ribonuclease activity associated with mammalian cell walls, Biochim. Biophys. Acta 155:353–359.PubMedGoogle Scholar
  125. Sulakhe, P. V., Fedelesova, M., McNamara, D. B., and Dhalla, N. S., 1971, Isolation of skeletal muscle membrane fragments containing active Na+-K+ stimulated ATPase: Comparison of normal and dystrophic muscle sarcolemma, Biochem. Biophys. Res. Commun. 42:793–800.PubMedCrossRefGoogle Scholar
  126. Takeuchi, M., and Terayama, H., 1965, Preparation and chemical composition of rat liver cell membranes, Exp. Cell Res. 40:32–44.PubMedCrossRefGoogle Scholar
  127. Thinès-Sempoux, D., Amar-Costesec, A., Beaufay, H., and Berthet, J., 1969, The association of cholesterol, 5′-nucleotidase, and alkaline phosphodiesterase I with a distinct group of microsomal particles, J. Cell Biol. 43:189–192.PubMedCrossRefGoogle Scholar
  128. Thuneberg, L., and Rostgaard, J., 1968, Isolation of brush border fragments from homogenates of rat and rabbit kidney cortex, Exp. Cell Res. 51:123–140.PubMedCrossRefGoogle Scholar
  129. Touster, O., Aronson, N. N., Jr., Dulaney, J. T., and Hendrickson, H., 1970, Isolation of rat liver plasma membranes: Use of nucleotide pyrophosphatase and phosphodiesterase I as marker enzymes, J. Cell Biol. 47:604–618.PubMedCrossRefGoogle Scholar
  130. Turkington, R. W., 1962, Thyrotropin-stimulated adenosine triphosphatase in isolated thyroid cell membranes, Biochim. Biophys. Acta 65:386–388.PubMedCrossRefGoogle Scholar
  131. Waite, M., and Sisson, P., 1973, Utilization of neutral glycerides and phosphotidylethanolamine by the phospholipase A1 of the plasma membranes of rat liver, J. Biol. Chem. 248:7985–7992.PubMedGoogle Scholar
  132. Warren, L., Glick, M. C., and Nass, M. K., 1967, Membranes of animal cells. I. Methods of isolation of the surface membrane, J. Cell. Physiol. 68:269–287.CrossRefGoogle Scholar
  133. Wattiaux-deConinck, A., and Wattiaux, R., 1969, Nucleosidediphosphatase activity in plasma membrane of rat liver, Biochim. Biophys. Acta 183:118–128.CrossRefGoogle Scholar
  134. Wattiaux-deConinck, S., Rutgeerts, M.-J., and Wattiaux, R., 1965, Lysosomes in ratkidney tissue, Biochim. Biophys. Acta 105:446–459.Google Scholar
  135. Weaver, R. A., and Boyle, W., 1969, Purification of plasma membranes of rat liver: Application of zonal centrifugation to isolation of cell membranes, Biochim. Biophys. Acta 173:377–388.PubMedCrossRefGoogle Scholar
  136. Weber, G., and Cantero, A., 1955, Glucose-6-phosphatase activity in normal, precancerous, and neoplastic tissues, Cancer Res. 15:105-108.Google Scholar
  137. Wetzel, M. G., and Korn, E. D., 1969, Phagocytosis of latex beads by Acanthamoeba castellanii (NEFF). III. Isolation of the phagocytic vesicles and their membranes, J. Cell Biol. 43:90–104.PubMedCrossRefGoogle Scholar
  138. Whittaker, V. P., 1968, The morphology of fractions of rat forebrain synaptosomes separated on continuous sucrose density gradients, Biochem. J. 106:412–417.PubMedGoogle Scholar
  139. Widnell, C. C., 1972, Cytochemical localization of 5′-nucleotidase in subcellular fractions isolated from rat liver. I. The origin of 5′-nucleotidase activity in microsomes, J. Cell Biol. 52:542–558.PubMedCrossRefGoogle Scholar
  140. Wilfong, R. F., and Neville, D. M., Jr., 1970, The isolation of a brush border membrane fraction from rat kidney, J. Biol. Chem. 245:6106–6112.PubMedGoogle Scholar
  141. Wolff, J., and Jones, A. B., 1971, The purification of bovine thyroid plasma membranes and the properties of membrane-bound adenyl cyclase, J. Biol. Chem. 246:3939–3947.PubMedGoogle Scholar
  142. Yamashita, K., and Field, J. B., 1970, Preparation of thyroid plasma membranes containing a TSH-responsive adenyl cyclase, Biochem. Biophys. Res. Commun. 40:171–178.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • David M. NevilleJr.
    • 1
  1. 1.Section on Biophysical Chemistry Laboratory of NeurochemistryNational Institute of Mental HealthBethesdaUSA

Personalised recommendations