Abstract
Classic laboratory techniques to diagnose coxsackievirus (CV)-induced diseases include isolation and typing of the causative agent from body fluids and/or demonstration of virus-specific antibodies in serum or cerebrospinal fluid (CSF) specimens.1,2 These techniques generally are powerful in the diagnosis of viral infections; however, in the case of CV, they are hampered by many difficulties.
Keywords
- Viral Antigen
- Laboratory Diagnosis
- Fast Protein Liquid Chromatography
- Nucleic Acid Hybridization
- eDNA Probe
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Melnick, J. L., Wenner, H. A., and Philips, C. A., 1979, Enteroviruses, in: Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections, 5th ed. (E. H. Lennette and N. J. Schmidt, eds.), pp. 471–534, American Public Health Association, Washington, D.C.
Melnick, J. L., 1982, Enteroviruses, in: Viral Infections of Humans, 2nd ed. (A. S. Evans, ed.), pp. 187–251, Plenum, New York.
Schmidt, N. J., Ho, H. H., and Lennette, E. H. 1975, Propagation and isolation of group A coxsackieviruses in RD cells, J. Clin. Microbiol. 2:183–185.
Wecker, I., and ter Meulen V., 1977, RD cells in the laboratory diagnosis of enteroviruses, Med. Microbiol. Immunol. 163:233–240.
Landry, M. L., Madore, H. P., Fong, C. K. Y., and Hsiung, G. D., 1981, Use of guinea pig embryo cell cultures for isolation and propagation of group A Coxsackie viruses, J. Clin. Microbiol. 13:588–593.
Schluederberg, A., 1965, Immune globulins in human viral infections, Nature (London) 205:1232–1233.
Schmidt, N.J., Lennette, E. H., and Dennis, J., 1968, Characterization of antibodies produced in natural and experimental coxsackievirus infections, J. Immunol. 100:99–106.
Schmidt, N. J., Magoffin, R. L., and Lennette, E. H., 1973. Association of group B coxsackieviruses with cases of pericarditis, myocarditis, or pleurodynia by demonstration of immunoglobulin M antibody, Infect. Immun. 8:341–348.
Svehag, S. E., and Mandel, B., 1963, The formation and properties of poliovirus neutralizing antibody, J. Exp. Med. 119:1–19.
Mertens, T., Hager, H., and Eggers, H. J., 1982, Epidemiology of an outbreak in a maternity unit of infections with an antigenic variant of echovirus 11, J. Med. Virol. 9:81–91.
Reiner, M., and Wecker, E., 1981, A modified absorption-reduction method to detect virus-specific hemagglutinin inhibiting and neutralizing IgM antibodies, Med. Microbiol. Immunol. 169:237–245.
Richman, D. D., 1983, The use of staphylococcal protein A in diagnostic virology, Curr. Top. Microbiol. Immunol. 104:159–176.
Beck, O. E., 1981, Distribution of virus antibody activity among human IgG subclasses, Clin. Exp. Immunol. 43:626–632.
Fahey, J. L., and Terry, E. W., 1978, Ion exchange chromatography and gel filtration, in: Handbook of Experimental Immunology, 3rd ed. (D. M. Weir, ed.), pp. 8.1–8.16, Blackwell Scientific Publications, Oxford.
Johnson, R. B., and Libby, R., 1980, Separation of immunoglobulin M (IgM) essentially free of IgG from serum for use in systems requiring assay of IgM-type antibodies without interference from rheumatoid factor. J. Clin. Microbiol. 12:451–454.
Sann, G., Schneider, G., Loeke, S., and Doerr, H. W., 1983, Rapid fractionation of serum immunoglobulins by high pressure liquid gel permeation chromatography. Applications to routine serologic procedures, J. Immunol. Methods 59:121–127.
Sampson, I. A., Hodgen, A. N., and Arthur, I. H., 1984, The separation of IgM from human serum by FPLC, J. Immunol. Methods 69:9–15.
Yolken, R. H., 1982, Enzyme immunoassay for the detection of infectious antigens in body fluids, Rev. Infect. Dis. 4:35–68.
Yolken, R. H., and Torsch, V., 1980, Enzyme-linked immunosorbent assay for the detection and identification of Coxsackie B antigen in tissue cultures and clinical specimens, J. Med. Virol. 6:45–52.
Yolken, R. H., and Torsch, V. M., 1981, Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A, Infect. Immun. 31:742–750.
Kulski, J. K., and Norval, M., 1985, Nucleic acid probes in diagnosis of viral diseases of man, Arch, Virol. 83:3–15.
Hyypiä, T., Stålhandske, P., Vainionpää, R., and Pettersson, U., 1984, Detection of enteroviruses by spot hybridization, J. Clin. Microbiol. 19:436–438.
Bowles, N. E., Richardson, P. J., Olsen, E. G. J., and Archard, L. C., 1986, Detection of coxsackie-B-virus-specific RNA sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy, Lancet 1:1120–1123.
Tracy, S., 1984, A comparison of genomic homologies among the coxsackievirus B group: Use of fragments of the cloned coxsackievirus B3 genome as probes, J. Gen. Virol 65:2167–2172.
Kandolf, R., Ameis, D., Kirschner, P., Canu, A., and Hofschneider, P. H., 1986, In situ detection of viral genomes in myocardial cells by nucleic acid hybridization: A novel approach to the diagnosis of viral heart disease, presented at the International Symposium on Inflammatory Heart Disease, May 15–17, Würzburg, Federal Republic of Germany.
Kandolf, R., and Hofschneider, P. H., 1985, Molecular cloning of the genome of a cardiotropic Coxsackie B3 virus: Full-length reverse-transcribed recombinant c-DNA generates infectious virus in mammalian cells, Proc. Natl. Acad. Sci. USA 82:4818–4822.
Prabhakar, B. S., Haspel, M. V., McClintock, P. R., and Notkins, A. L., 1982, High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies, Nature (Lond.) 300:374–376.
Prabhakar, B. S., Menegus, M. A., and Notkins, A. L., 1985. Detection of conserved and nonconserved epitopes on coxsackievirus B4: Frequency of antigenic change, Virology 146:302–306.
Cao, Y., Schnurr, D. P., and Schmidt, N. J., 1984, Monoclonal antibodies for study of antigenic variation in coxsackievirus type B4: Association of antigenic determinants with myocarditic properties of the virus, J. Gen. Virol. 65:925–935.
Pattison, J. R., 1983, Tests for Coxsackie B virus-specific IgM, J. Hyg. (Lond.) 90:327–332.
Dörries, R., and ter Meulen, V., 1980, Detection of enterovirus specific IgG and IgM antibodies in humans by an indirect solid phase radioimmunoassay, Med. Microbiol. Immunol. 168:159–171.
Dörries, R., and ter Meulen V., 1983, Specificity of IgM antibodies in acute human coxsackievirus B infections, analysed by indirect solid phase enzyme immunoassay and immunoblot technique, J. Gen. Virol. 64:159–167.
El-Hagrassy, M. M. O., and Banatvala, J. E., 1980, Coxsackie-B-virus-specific IgM responses in patients with cardiac and other diseases, Lancet 2:1160–1162.
Chan, D., and Hammond, G. W., 1985, Comparison of serodiagnosis of group B coxsackievirus infections by an immunoglobulin M capture enzyme immunoassay versus microneutralization, J. Clin. Microbiol. 21:830–834.
McCartney, R. A., Banatvala, J. E., and Bell, E. J., 1986, Routine use of μ-antibody-capture ELISA for the serological diagnosis of Coxsackie B virus infections, J. Med. Virol. 19:205–212.
Bell, E. J., McCarney, R. A., Basquill, D., and Chaudhuri, A. K. R., 1986, μ-Antibody capture ELISA for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis, J. Med. Virol. 19:213–217.
Meurman, O., 1983, Detection of antiviral IgM antibodies and its problems—A review, Curr. Top. Microbiol. Immunol. 104:101–131.
Ziegler, D. W., 1979, Determination of IgM antibodies in diagnostic virology, in: Diagnosis of Viral Infecfions (D. A. Lenette, S. Pecter, and K. D. Thompson, eds.), pp. 63–71, University Park Press, Baltimore.
Salonen, E.-M., Vaheri, A., Suni, J., and Wager, O., 1980, Rheumatoid factor in acute viral infections: Interference with determination of IgM, IgG and IgA antibodies in an enzyme immunoassay, J. Infect. Dis. 142:250–255.
Hannington, G., Booth, J. C., Wiblin, C. N., and Stern, H., 1983, Indirect enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies against Coxsackie B viruses, J. Med. Microbiol. 16:459–465.
Pugh, S. F., 1984, Heterotypic reactions in a radioimmunoassay for Coxsackie B virus specific IgM, J. Clin. Pathol. 37:433–439.
Morgan-Capner, P., and McSorley, C., 1983, Antibody capture radioimmunoassay (MACRIA) for coxsackievirus B4 and B5-specific IgM, J. Hyg. (Lond.) 90:333–349.
Banatvala, J. E., Bryant, J., Schernthaner, G., Borkenstein, M., Schober, E., Brown, D., De Silva, L. M., Menser, M. A., and Silink, M., 1985, Coxsackie B, mumps, rubella and cytomegalovirus specific IgM responses in patients with juvenile-onset insulin-dependent diabetes mellitus in Britain, Austria and Australia, Lancet 1:1409–1412.
Mertens, Th., Pika, U., and Eggers, H. J., 1983, Cross antigenicity among enteroviruses as revealed by immunoblot technique, Virology 129:431–442.
Katze, M. G., and Crowell, R. L., 1980, Immunological studies of the group B coxsackieviruses by the sandwich enzyme-linked immunosorbent assay (ELISA) and im-munoprecipitation, J. Gen. Virol. 50:357–367.
Eggers, H. J., and Mertens, T., 1986, Persistence of Coxsackie B virus-specific IgM, Lancet 2:284.
Tilzey, A. T., Signy, M., and Banatvala, J. E., 1986, Persistent Coxsackie B virus specific IgM response in patients with recurrent pericarditis, Lancet 1:1491–1492.
Halonen, P., Meurman, O., Lövgren, T., Hemmilä, I., and Soini, E., 1983, Detection of viral antigens by time-resolved fluoroimmunoassay Curr. Top. Microbiol. Immunol. 104:133–146.
Boguslawski, S. J., Smith, D. E., Michalak, M. A., Mickelson, K. E., Yehle, C. O., Patterson, W. L., and Carrico, R. J., 1986, Characterization of monoclonal antibody to DNA-RNA and its application to immunodetection of hybrids, J. Immunol. Methods 89:123–130.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Plenum Press, New York
About this chapter
Cite this chapter
Dörries, R. (1988). New Approaches to Laboratory Diagnosis. In: Bendinelli, M., Friedman, H. (eds) Coxsackieviruses. Infectious Agents and Pathogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0247-7_12
Download citation
DOI: https://doi.org/10.1007/978-1-4757-0247-7_12
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-0249-1
Online ISBN: 978-1-4757-0247-7
eBook Packages: Springer Book Archive