Methods to Investigate the Function of Certain Amino Acids in the CNS

  • N. M. van Gelder
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 1)


Aside from participating in metabolic processes, certain amino acids in the CNS may serve a more specialised physiological purpose in one of the three ways proposed below which, it should be remembered, are however not mutually exclusive. Thus an amino acid serving a certain role in the CNS, may well be used in a somewhat altered function by a specialised tissue such as the retina.


Nerve Terminal Amino Acid Concentration Label Amino Acid Active Amino Acid Anatomical Pathway 
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  1. Bennett, J.P., Logan, W.J. and Snyder, S.H. (1973) Amino acids as central nervous transmitters: The influence of ions, amino acid analogues and ontogeny on transport systems for L-glutamic and L-aspartic acids and glycine into central nervous synapto-somes of the rat. J. Neurochem. 21: 1533–1550.PubMedCrossRefGoogle Scholar
  2. Bowery, N.G. and Brown, D.A. (1972) y-Ami-nobutyric acid uptake by sympathetic ganglia. Nature New Biol. 238: 89–91.PubMedCrossRefGoogle Scholar
  3. Bradford, H.F., Bennett, G.W. and Thomas, A.J. (1973) Depolarizing stimuli and the release of physiologically active amino acids from suspensions of mammalian synaptosomes. J. Neurochem. 21: 495–505.PubMedCrossRefGoogle Scholar
  4. Buu, N.T. and van Gelder, N.M. (1974) Differences in biochemical properties of γ-inobutyric acid aminotransferase from synap-tosome-enriched and cytoplasmic mitochondria-enriched subcellular fractions of mouse brain. Can. J. Physiol. Pharmacol. 52: 674–680.PubMedCrossRefGoogle Scholar
  5. Changeux, J.P., Kasai, M. and Lee, C.-Y. (1970) Use of snake venom toxin to characterize the cholinergic receptor protein. Proc. Natl. Acad. Sci. U.S., 67: 1241–1247.CrossRefGoogle Scholar
  6. Cohen, A.I., McDaniel, M. and Orr, H. (1973) Absolute levels of some free amino acids in normal and biologically fractionated retinas. Invest. Opthalm. 12: 686–693.Google Scholar
  7. Collins, G.G.S. (1974) The spontaneous and electrically-evoked release of 3H -GABA from the isolated hemisected frog spinal cord. Brain Res. 66: 121–137.CrossRefGoogle Scholar
  8. Curtis, D.R. and Watkins, J.C. (1963) Acidic amino acids with strong excitatory actions on mammalian neurones. J. Physiol. (Lond.) 166: 1–14.Google Scholar
  9. Davidoff, R.A., Graham, L.T., Shank, R.P., Werman, R. and Aprison, M.H. (1967) Changes in amino acid concentrations associated with loss of spinal interneurons. J. Neurochem. 13: 1025–1031.CrossRefGoogle Scholar
  10. Douglas, W.W. and Rubin, R.P. (1961) The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J. Physiol. 159: 40–57.PubMedGoogle Scholar
  11. Ehinger, B. (1972) Cellular location of the uptake of some amino acids in the rabbit retina. Brain Res. 46: 297–311.PubMedCrossRefGoogle Scholar
  12. Ehinger, B. (1973) Glial uptake of taurine. Brain Res. 60: 512–516.PubMedCrossRefGoogle Scholar
  13. Elliott, K.A.C. and van Gelder, N.M. (1958). Occlusion and metabolism of γ-aminobutyric acid by brain tissue. J. Neurochem. 3: 28–40.PubMedCrossRefGoogle Scholar
  14. Fonnum, F. and Walberg, F. (1973) An estimation of the concentration of y-aminobutyric acid and glutamate decarboxylase in the inhibitory Purkinje axon terminals in the cat. Brain Res. 54: 115–127.PubMedCrossRefGoogle Scholar
  15. Gerschenfeld, H.M. (1973) Chemical transmission in invertebrate central nervous systems and neuromuscular junctions. Physiol. Review 53:1–119.Google Scholar
  16. Hammerstead, J.P. and Cutler, R.W.P. (1972) Sodium ion movements and the spontaneous and electrically-stimulated release of 3h-GABA and 14C-glutamic acid from rat cortical slices. Brain Res. 47: 401–413.CrossRefGoogle Scholar
  17. Henn, F.A. and Hamberger, A. (1971) Glial cell function. Uptake of transmitter substances. Proc. Nat. Acad. Sci. 68: 2686–2690.PubMedCrossRefGoogle Scholar
  18. Iversen, L.L. (1970) Neurotransmitters, neurohormones and other small molecules in neurons. In: The Neurosciences, Second Study Program, edited by Francis O. Schmidt, Rockefeller Univ. Press, N.Y., pp. 768–782.Google Scholar
  19. Jasper, H.H. and Koyama, I. (1969) Rate of release of amino acids from the cerebral cortex in the cat as affected by brainstem and thalamic stimulation. Can. J. Physiol. Pharmacol. 47: 889–905.PubMedCrossRefGoogle Scholar
  20. Katz, B. and Miledi, R. (1967) The timing of calcium action during neuromuscular transmission. J. Physiol. 189: 535–544.PubMedGoogle Scholar
  21. Kennedy, A.J. and Voaden, M.J. (1974) Factors affecting the spontaneous release of 3H-yaminobutyric acid from the frog retina in vitro. J. Neurochem. 22: 63–71.PubMedCrossRefGoogle Scholar
  22. Krnjevic, K. (1974) Chemical nature of synaptic transmission in vertebrates. Physiol. Review 54: 418–540.Google Scholar
  23. Levi, G. and Raiteri, M. (1973) GABA and glutamate uptake by subcellular fractions enriched in synaptosomes: critical evaluation of some methodological aspects. Brain Res. 57: 165–185.PubMedCrossRefGoogle Scholar
  24. Levin, E., Garcia Argiz, C.A. and Nogueira, G.T. (1966) Ventriculo-cisternal perfusion of amino acids in cat brain. J. Neurochem. 13: 979–988.PubMedCrossRefGoogle Scholar
  25. Martin, D.L. and Smith, A.A. (1972) Ions and the transport of gamma-aminobutyric acid by synaptosomes. J. Neurochem. 19: 841–855.PubMedCrossRefGoogle Scholar
  26. Mcllwain, H., Harvey, J.A. and Rodriguez, G. (1969) Tetrodotoxin on the sodium and toehr ions of cerebral tissues, excited electrically or with glutamate. J. Neurochem. 16: 363–370.CrossRefGoogle Scholar
  27. Miledi, R., Molinoff, P. and Potter, L.T. (1971) Isolation of the cholinergic receptor protein of Torpedo electric tissue. Nature (Lond.), 229: 554–557.CrossRefGoogle Scholar
  28. Molinoff, P.B. and Kravitz, E.A. (1967) The metabolism of y-amino-butyric acid (GABA) in the lobster nervous system — glutamate decarboxylase. J. Neurochem. 15: 391–409.CrossRefGoogle Scholar
  29. Neal, M.J. and Iversen, L.L. (1972) Autoradiographic localisation of 3H-GABA in rat retina. Nature New Biol. 235: 217–218.PubMedCrossRefGoogle Scholar
  30. Neal, M.J. and Starr, M.S. (1973) Effect of inhibitors of γ-amino-butyrate aminotransferase on the accumulation of 3H-y-aminobuty-ric acid by the retina. Br. J. Pharmac. 47: 543–555.CrossRefGoogle Scholar
  31. Otsuka, M. and Miyata, Y. (1972) Application of enzymatic cycling to measurement of gamma-aminobutyric acid in single neurons of the mammalian nervous system. Advances in Biochem. Psychophar-macol. 6: 61–74.Google Scholar
  32. Pardee, A.B. (1968) Membrane transport proteins. Science, 162: 632–637.PubMedCrossRefGoogle Scholar
  33. Pasantes-Morales, H., Klethi, J., Urban, P.F. and Mandel, P. (1974). The effect of electrical stimulation, light and amino acids on the efflux of 35S -taurine from retina of domestic fowl. Exp. Brain Res. 19: 131–141.PubMedCrossRefGoogle Scholar
  34. Pitot, H.C. and Yatvin, M.B. (1973) Interrelationship of mammalian hormones and enzyme levels in vivo. Physiol. Review 53: 228–325.Google Scholar
  35. Riggs, T.R., Pan, M.W. and Feng, H.W. (1972) Transport of amino acids into estrogen-primed uterus: III: Effect of the level of extracellular sodium ion. J. Biol. Chem. 247: 7128–7134.PubMedGoogle Scholar
  36. Saito, K., Barber, R., Wu, J.-Y., Vaughn, J.E. and Roberts, E. (1974) Immunohistochemical localisation of glutamic acid decarboxylase in rat central nervous system at light microscopic level. Proc. Am. Soc. Neurochem. 5: 113–114.Google Scholar
  37. Schon, F. and Kelly, J.S. (1974a) Autoradiographic localisation of [3H] GABA and [3H] glutamate over satellite glial cells. Brain Res. 66: 275–288.CrossRefGoogle Scholar
  38. Snodgrass, S.R. and Lorenzo, A.V. (1973) Transport of GABA from the perfused ventricular system of the cat. J. Neurochem. 20: 761–769.PubMedCrossRefGoogle Scholar
  39. Starr, M.S. (1973) Effects of changes in the ionic composition of the incubation medium on the accumulation and metabolism of 3H-γ-amino-butyric acid and -taurine in isolated rat retina. J. Neurochem. 22: 1693–1700.Google Scholar
  40. Takeuchi, A. and Takeuchi, N. (1966) A study of the inhibitory action of y-aminobutyric acid on neuromuscular transmission in the crayfish. J. Physiol. 183: 418–432.PubMedGoogle Scholar
  41. Van Gelder, N.M. (1965) A comparison of y-aminobutyric acid metabolism in rabbit and mouse nervous tissue. J. Neurochem. 12: 239–244.CrossRefGoogle Scholar
  42. Van Gelder, N.M. (1971) Molecular arrangement for physiological action of glutamic acid and y-aminobutyric acid. Can. J. Physiol. and Pharm. 49: 513–519.CrossRefGoogle Scholar
  43. Voaden, M.J., Marshall, J. and Murani, N. (1974) The uptake of [3h] γ-aminobutyric acid and [3h] glycine by the isolated retina of the frog. Brain. Res. 67: 115–132.PubMedCrossRefGoogle Scholar
  44. Werman, R. (1966) Criteria for identification of a central nervous system transmitter. Comp. Biochem. Physiol. 18: 745–766.PubMedCrossRefGoogle Scholar
  45. Zieglgänsberger, W. and Puil, E.A. (1973) Action of glutamic acid on spinal neurons. Exp. Brain. Res. 17: 35–49.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • N. M. van Gelder
    • 1
  1. 1.Groupe de Recherche en Sciences Neurologiques du Conseil Médical du Canada, Département de PhysiologieUniversité de MontréalCan.

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