Molecular Characterization of Allergens of Kentucky Bluegrass Pollen

  • Egil Olsen
  • Ming Yang
  • Shyam S. Mohapatra


Grass pollen is one of the major causes of allergy worldwide and since the 1960s many different grass species have been extensively studied for their allergenic proteins by biochemical and immunological methods. Grass pollen extracts are complex mixtures of allergenic and nonallergenic proteins and may consist of up to 14 different IgE-binding components (Ford and Baldo, 1986). Several allergens have been defined and partly characterized, but only a few have been isolated in pure form from pollen extract (Ansari et al., 1989a, b). Comparison of pollen proteins from different grass species have revealed considerable cross-reactivity (Leiferman and Gleich, 1986; Bernstein et al., 1976). Employing monoclonal antibodies, groups of allergens have been defined with interspecies cross-reactivity (Standring et al., 1987; Singh and Knox, 1985). Because of the difficulties in isolating sufficient amounts of pure allergens from pollen extracts, the availability of structural information of grass allergens was limited until recombinant DNA techniques were introduced (Fang et al., 1988).


Grass Pollen Pollen Allergen Pollen Extract Major Allergen Kentucky Bluegrass 
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  1. Ansari, A. A., P. Shenbagamurthi, and D. G. Marsh. 1989a. Complete amino acid sequence of a Lolium perenne (perennial rye grass) pollen allergen, Lol p II. J. Biol. Chem. 264:11181.PubMedGoogle Scholar
  2. Ansari, A. A., P. Shenbagamurthi, and D. G. Marsh. 1989b. Complete primary structure of a Lolium perenne (perennial rye grass) pollen allergen, Lol p III: Comparison with known Lol pI and II sequences. Biochemistry 28:8665.PubMedCrossRefGoogle Scholar
  3. Astwood, J., S. S. Mohapatra, H. Ni, and R. D. Hill. 1995. Barley allergen homologous protein in crop plants. Clin. Exp. Allergy 25:66.PubMedCrossRefGoogle Scholar
  4. Atassi, M. Z. 1984. Antigenic structures of proteins. Eur. J. Biochem. 145:1.PubMedCrossRefGoogle Scholar
  5. Baldo, B. A., R. C. Panzani, D. Bass, and R. Zerboni. 1992. Olive and privet pollen allergens. Identification and cross reactivity with grass pollen proteins. Mol. Immunol. 29:1209.PubMedCrossRefGoogle Scholar
  6. Baskar, S., P. Parronchi, S. S. Mohapatra, S. Romagnani, and A. A. Ansari. 1992. Human T cell responses to the purified pollen allergens of the grass, Lolium perenne: relationship between structural homology and T cell recognition. J. Immunol. 148:2378.PubMedGoogle Scholar
  7. Bernstein, I. L., M. Perera, J. Gallagher, J. G. Michael, and S.G.O. Johansson. 1976. In vitro cross-reactivity of major aeroallergenic pollens by the radioallergosorbent technique. J. Allergy Clin. Immunol. 57:141.PubMedCrossRefGoogle Scholar
  8. D’Amato, G., R. De-Palma, A. Verga, P. Martucci, and G. Labefalo. 1991. Antigenic activity of non-pollen parts (leaves and stems) of allergenic plants (Parietaria judaica and Dactylis glomerata). Ann. Allergy 67:421.PubMedGoogle Scholar
  9. Ebner, C., F. Ferreira, K. Hoffman, R. Hirschwehr, S. Schenk, Z. Szebfalusi, H. Breiteneder, P. Parronchi, S. Romagnani, O. Scheiner and D. Kraft. 1993. T cell clones specific for Bet V1, the major birch pollen allergen, crossreact with major allergens of hazel, coral and alder. Mol. Immunol. 30:1323.PubMedCrossRefGoogle Scholar
  10. Esch, R. E. and D. G. Klapper. 1989. Isolation and characterization of a major cross-reactive grass group I allergenic determinant. Mol. Immunol. 26:557.PubMedCrossRefGoogle Scholar
  11. Fang, K.S.Y, M. Vitale, P. Fehlner, and T. P. King. 1988. cDNA cloning and primary structure of a white-face hornet venom allergen, antigen 5. Proc. Natl. Acad. Sci. USA 85:895.PubMedCrossRefGoogle Scholar
  12. Ford, S. A. and B. A. Baldo. 1986. A re-examination of ryegrass (Lolium perenne) pollen allergens. Internat. Arch. Allergy Appl. Immunol. 81:193.CrossRefGoogle Scholar
  13. Guo, L., P. P. Stepien, J. Yuon Tso, R. Brousseau, S. Narang, D. Y. Thomas, and R. Wu. 1984. Synthesis of insulin gene. Gene 29:251.PubMedCrossRefGoogle Scholar
  14. Klysner, S., F. Matthiesen, and H. Lowenstein. 1990. Affinity purification of grass pollen group V allergens using monoclonal antibodies. J. Allergy Clin. Immunol. 85:279.Google Scholar
  15. Leiferman, K. M. and G. J. Gleich. 1976. The cross-reactivity of IgE antibodies with pollen allergens. I. Analysis of various species of grass pollen. J. Allergy Clin. Immunol. 58:129.PubMedCrossRefGoogle Scholar
  16. McCormick, S., D. Twell, G. Vaneanneyt, and J. Yamaguchi. 1991. Molecular analysis of gene regulation and function during male gametrophyte development. Symp. Soc. Exp. Biol. 45:229.PubMedGoogle Scholar
  17. Mohapatra, S.S. 1992. Diagnosis and therapy of IgE-mediated allergies with recombinant allergens: Poa pratensis IX allergen as a model. Pharmacia Allergy Research Foundation Awards Book, ed. M. Debelic, pp. 4–15.Google Scholar
  18. Mohapatra, S. S., R. Hill, J. Astwood, A.K.M. Ekramoddoullah, E. Olsen, A. Silvano-vitch, T. Hatton, T. T. Kisil, and A. H. Sehon. 1990a. Isolation and characterization of a cDNA clone encoding an IgE binding protein from Kentucky Bluegrass (Poa pratensis) pollen. Internat. Arch. Allergy Appl. Immunol. 91:362.CrossRefGoogle Scholar
  19. Mohapatra, S. S., R. D. Hill, and A. H. Sehon. 1990b. Molecular cloning of allergens: progress and perspectives. Aerobiologia 6: 205.CrossRefGoogle Scholar
  20. Mohapatra, S. S. 1993. Structural motifs as a basis of cross-reactivity among pollen allergens. In Proc. Int. Symp. Molecular Biology and Immunology of Allergens, eds. D. Kraft and A. H. Sehon, pp. 69–81. CRC Press, Boca Raton, FL.Google Scholar
  21. Mohapatra, S. S. 1994. Molecular characterization of pollen allergens: Implications for immunotherapy. In: Recent trends in Aerobiology, Allergy and Immunology, Agashe, S. N. (ed). Oxford IBH, New Delhi, pp. 278–292.Google Scholar
  22. Mohapatra, S. S. and A. H. Sehon. 1992. Therapeutic potential of recombinant allergens. Internat. Arch Allergy Immunol. 98:265.CrossRefGoogle Scholar
  23. Mohapatra, S. S., S. Mohapatra, M. Yang, A. A. Ansari, P. Parronchi, E. Maggi, and S. Romagnani. 1994. Molecular basis of cross-reactivity among allergen-specific human T cells: T cell receptor gene usage and epitope structure. Immunology 81:15.PubMedGoogle Scholar
  24. Mohapatra, S. S., C. F. Nicodemus, C. Schou, and R. Valenta. 1994. Recombinant allergens and epitopes. Allergy and Clin. Immunol. News 6:45.Google Scholar
  25. Mourad, W., G. Pelletier, A. Boulet, N. Islam, J.-P. Valet, and J. Hebert. 1986. Allergenicity and cross-reactivity of rye grass pollen extracts revealed by monoclonal antibodies. J. Immunol. Methods 89:53.PubMedCrossRefGoogle Scholar
  26. Olsen, E. and S. S. Mohapatra. 1992. Expression and Thrombin cleavage of Poa p IX recombinant allergens fused to glutathione S-transferase. Internat. Arch. Allergy Appl. Immunol. 98:343.CrossRefGoogle Scholar
  27. Olsen, E., L. Zhang, R. D. Hill, F. T. Kisil, A. H. Sehon, and S. S. Mohapatra. 1991. Identification and characterization of the Poa p IX group of basic allergens of Kentucky bluegrass pollen. J. Immunol. 147: 205.PubMedGoogle Scholar
  28. Olsen, E., A. Fallang, and S. S. Mohapatra. 1995. Characterization of antibody responses to grass pollen allergens in non-atopic and atopic individuals with sensitization to grass and tree allergens. Allergy (in press).Google Scholar
  29. Perez, M., G. V. Ishioka, L. E. Walker, and R. W. Chesnut. 1990. cDNA cloning and immunological characterization of the ryegrass allergen Lol p I. J. Biol. Chem. 265:16210.PubMedGoogle Scholar
  30. Rafnar, T., I. G. Griffith, M. C. Kuo, J. F. Bond, B. L. Rogers, and D. G. Klapper. 1991. Cloning of Arab a I (antigen E), the major allergen family of short ragweed pollen. J. Biol Chem. 266:1229.PubMedGoogle Scholar
  31. Silvanovich, A., J. Astwood, E. Olsen, L. Zhang, F. Kisil, A. Sehon, S. Mohapatra, and R. Hill. 1991. Nucleotide sequence analysis of three cDNA’s coding for major ispallergens of Kentucky bluegrass pollen. J. Biol. Chem. 266:1204.PubMedGoogle Scholar
  32. Singh, M. B. and R. B. Knox. 1985. Grass pollen allergens: Antigenic relationships detected using monoclonal antibodies and dot blotting immunoassay. Internat. Arch. Allergy Appl. Immunol. 78:300.CrossRefGoogle Scholar
  33. Smith, D. B. and K. S. Johnson. 1988. Single step purification of polypeptides in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31.PubMedCrossRefGoogle Scholar
  34. Sriramarao, P., and P. V. Subba Rao. 1993. Allergenic cross-reactivity between Parthenium and ragweed pollen allergens. Int. Arch. Allergy and Immunology. 100:79.CrossRefGoogle Scholar
  35. Standring, R., V. Spackman, and S. J. Porter. 1987. Distribution of a major allergen of Rye grass (Loliumperenne) pollen between other grass species. Internat. Arch. Allergy Appl. Immunol. 83:96.CrossRefGoogle Scholar
  36. Takahasi, Y., S. Katagiri, and F. Ewapo. 1987. Analysis of cross reactivities among fruit pollen allergens. Areug 36:377.Google Scholar
  37. Thomas, W. R., G. A. Stewart, R. J. Simpson. 1988. Cloning and expression of DNA coding for the major house dust mite allergen, Der p I, in Escerichia coli. Internat. Arch. Allergy Appl. Immunol. 85:127.Google Scholar
  38. Tovey, E. R., M. C. Johnson, A. L. Roche, G. S. Cobon, and B. A. Baldo. 1989. Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen. J. Exp. Med. 170:1457.PubMedCrossRefGoogle Scholar
  39. Yang, M., E. Olsen, J. Dolovich, A. H. Sehon, and S. S. Mohapatra. 1991. Immunological characterization of a recombinant Kentucky bluegrass (Poa pratensis) allergenic peptide. J. Allergy Clin. Immunol. 87:1096.PubMedCrossRefGoogle Scholar
  40. Zhang, L., F. T. Kisil, A. H. Sehon, and S. S. Mohapatra. 1991. Allergenic and antigenic cross-reactivities of Group IX grass pollen allergens. Internat. Arch. Allergy Appl. Immunol. 96:28.CrossRefGoogle Scholar
  41. Zhang, L., E. Olsen, R. Hill, F. Kisil, A. Sehon, and S. S. Mohapatra. 1992. Mapping of B cell epitopes of a recombinant Poa p IX allergen of Kentucky Bluegrass pollen. Mol. Immunol. 29:1383.PubMedCrossRefGoogle Scholar

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© Chapman & Hall 1996

Authors and Affiliations

  • Egil Olsen
  • Ming Yang
  • Shyam S. Mohapatra

There are no affiliations available

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