Magnetic Reactivation of Partially Inhibited Trypsin
The preliminary observations by Cook1 and Smith2 (see also the previous chapter) of changes in the activity of trypsin following exposure to a static magnetic field have been reinvestigated. Under similar, but more precisely defined conditions, we have observed that partially inhibited trypsin preparations show a 4–12% restoration of activity. Reactivation has been observed in our experiments following deactivation by egg-white trypsin inhibitor and apparently also following deactivation by 1–3 hr autolysis at pH 7–8 in the absence of calcium ions. No reactivation was observed after deactivation by ultraviolet exposure or treatment with soy-bean trypsin inhibitor or diisopropylphosphoro-fluoridate (DPF). Reactivation of the inhibited preparation following exposure to the magnetic field in no case exceeded the usual activity level of the uninhibited enzyme. The maximum reactivation (9–12$) followed the longest magnetic exposures (1008–1106 min). An exposure of 90 min coupled with slight (4$) inhibition appears to be insufficient.
KeywordsHydrolysis Quartz Mercury Trypsin
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- 1.Cook, Elton, paper presented at the 143rd meeting of the American Chemical Society, January 14, 1963; discussion found in Chem. Eng. News 41:44, January 21, 1963.Google Scholar
- 2.Smith, Sister M. Justa, and Elton S. Cook, paper presented at 2nd Biomagnetic Symposium, Chicago, Illinois, November 29, 1963.Google Scholar