Advertisement

Membrane Inlets for on-Line Liquid-Phase Mass Spectrometric Measurements in Bioreactors

  • Raimond Peter Cox

Summary

A simple type of sterilisable membrane-covered inlet to a mass spectrometer consists of silicone tubing covering small circular holes in a metal tube. An inlet of this type allows continuous measurements of the concentrations of dissolved gases and hydrophobic compounds such as alcohols in bioreactors. The measuring system has been used to follow ethanol, oxygen and carbon dioxide during the production of yeast biomass from molasses on the laboratory and industrial scale.

Keywords

Membrane Material Silicone Tubing Yeast Biomass Scale Fermenter Mass Spectrometer Inlet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bohatka, S., 1985, Quadrupole mass spectrometric measurement of dissolved and free gases, in “Gas Enzymology”, H. Degn, R. P. Cox and D. Lloyd, eds., Reidel, Dordrecht.Google Scholar
  2. Brodbelt, J. S. and Cooks, R. F., 1985, An exceedingly simple mass spectrometer interface with application to reaction monitoring and environmental analysis. Anal. Chem. 57:1155.Google Scholar
  3. Degn, H., Cox, R. P. and Lloyd, D., 1985, Continuous measurements of dissolved gases in biochemical systems with the quadrupole mass spectrometer, Meth. Biochem. Anal. 31:165.Google Scholar
  4. Heinzle, E., Furukawa, K., Dunn, I. J. and Bourne, J. R., 1983, Experimental methods for on-line mass spectrometry in fermentation technology, Bio/Technology 1:181.CrossRefGoogle Scholar
  5. Hoch, G. and Kok, B., 1963, A mass spectrometer inlet system for sampling gases dissolved in liquid phases, Arch. Biochem. Biophys. 101:160.Google Scholar
  6. Lloyd, D., Bohatky, S. and Szilagyi, J., 1985, Quadrupole mass spectrometry in the monitoring and control of fermentations, Biosensors 1: 179.Google Scholar
  7. Pungor, E., Jr., Schaefer, E. J., Cooney, C. L. and Weaver, J. C., 1983, Direct monitoring of the liquid and gas phases during a fermentation in a computer-massGoogle Scholar
  8. spectrometer-fermentor system, Eur. T. Appl. Microbiol. Biotechnol. 18:135.Google Scholar
  9. Reuss, M., Piehl, H., and Wagner, F., 1975, Application of mass spectrometry to the measurement of dissolved gases and volatile substances in fermemtation, Eur. T. Appl. Microbiol. 1:323.Google Scholar
  10. Schmidt, W. J., Meyer, H.D., Schuegerl, K. Kuhlmann, W., and Belgardt, K.-H., 1984, On-line analysis of fermentation media, Anal. Chim. Acta 163: 101.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Raimond Peter Cox
    • 1
  1. 1.Institute of BiochemistryOdense UniversityOdense MDenmark

Personalised recommendations