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Development of an On-Line Pyrolysis Mass Spectrometry System for the On-Line Analysis of Fermentations

  • E. P. Sandmeier
  • J. Keller
  • E. Heinzle
  • I. J. Dunn
  • J. R. Bourne

Abstract

Mass spectrometry has been used already in a number of ways to analyse fermentations:
  • with a capillary inlet for gas measurement, to monitor the feed and the exhaust gas concentrations, thus allowing gas balances over the reactor to be established (Heinzle et al., 1984)

  • with a membrane inlet as a probe for volatile compounds dissolved in fermentation broth (Reuss et al., 1975; Weaver, 1982; Bohatka et al., 1983; Heinzle et al., 1985a)

  • to characterize states of a fermentation using off-line pyrolysis mass spectrometry and factorial analysis (Heinzle et al.,1985b; Meuzelaar et aí.,1982)

There is, however, no good method existing to routinely analyse non volatiles in fermentation liquid using mass spectrometry. The main problem is to reproducably introduce such compounds into the high vacuum of the mass spectrometer. The aim of the presented work was to develop an analytical method based on pyrolysis mass spectrometry to monitor on-line concentrations of non-volatile components in a fermentation system.

Keywords

Fermentation Broth Ionization Chamber Pyrolysis Reactor Secondary Electron Multiplier Pyrolysis Mass Spectrometry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bohatka, S., Langer, G., Szilagyi, J. and Berecz, I., 1983, Gas Concentration Determination in Fermentors with Quadrupole Mass Spectrometer, Int. T. Mass Spectrom. Ion Phys., 48: 277–280.CrossRefGoogle Scholar
  2. Heinzle, E., Moes, J., Griot, M., Kramer, H., Dunn, I.J., Boume, J.R., 1984, On-line Mass Spectrometry in Fermentations, Anal. Chimica Acta 163: 219–229.CrossRefGoogle Scholar
  3. Heinzle, E., Kramer, H., Dunn, I.J., 1985a, State Analysis of Fermentation using a Mass Spectrometer with Membrane Probe. Biotechnol. Bioeng., 27: 238–246.CrossRefGoogle Scholar
  4. Heinzle, E., Lunden, M., Dunn, I.J., 1985b, Analysis of Biomass and Metabolites using Pyrolysis Mass Spectrometry, in: “Modelling and Control of Biotechnological Processes”, 1st IFAC Symposium, A. Johnson, ed., Pergamon Press, Oxford.Google Scholar
  5. Meuzelaar, H.L.C., Haverkamp, J., Hileman, F.D., 1982, Pyrolysis Mass Spectrometry of Recent and Fossil Biomaterials, Elsevier, Amsterdam.Google Scholar
  6. Weaver, J.C., 1982, Continuous Monitoring of Volatile Metabolites by a Mass Spectrometer, in: “Noninvasive Probes of Tissue Metabolism”, J.S. Cohen, ed., J. Wiley & Sons, N.Y.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • E. P. Sandmeier
    • 1
  • J. Keller
    • 1
  • E. Heinzle
    • 2
  • I. J. Dunn
    • 1
  • J. R. Bourne
    • 1
  1. 1.Chemical Engeneering Department (TCL)Swiss Federal Institute of Technology (ETH)ZurichSwitzerland
  2. 2.Laboratorium für Biotechnologie and Biochemie (LBB)GrazAustria

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