Differentiation of Phage Sensitive and Phage Resistant Streptococcus Cremoris Strains by Pyrolysis Mass Spectrometry and Discriminant Analysis of the Cell Walls
Cell wall preparations of variants from four different Streptococcus cremoris strains R1, 4, 10 and 40 were investigated by classical microbiological techniques and by pyrolysis mass spectrometry combined with multivariate analysis. Discriminant analytical data correlated highly with plaque forming ability, pointing to chemical differences between the cell walls of phage sensitive and phage resistant variants.
Pyrolysis gas chromatographic mass spectrometric data on a wild type strain (10) and one of its phage resistant variants confirmed the differences in mass peak distribution in the PYMS data and allowed the extrapolation to the chemistry of the cell wall. Incubation of the strains with their homologous phages selects for phage resistant variants with a markedly different ratio between peptidoglycan and rhamnose rich nonpeptidoglycan polymers. A protection of the receptor sites on the cell walls of phage resistant variants by their nonpeptidoglycan polymers is proposed as the main mechanism of phage resistance.
KeywordsPyrolysis Product Mass Peak Mass Chromatogram Analytical Pyrolysis Cell Wall Preparation
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