The Use of Mass Spectrometry in the Screening of Microorganisms on Productivity
Quantitative measurement of volatile compounds in a complex matrix may cause serious analytical problems. In this case the production of ethanol (p.p.m. level) in an environment already containing ethanol (percentages), had to be measured.
Using the isotope dilution method GC-MS failed due to chemical noise and the lack of high resolution (resolution power needed was 40.000). This article describes a fast GC-MS-MS method, using a simple, medium resolution double focussing mass spetrometer. This method has the advantage that no sample pretreatment is necessary, meaning the whole analysis may be performed in less than 5 minutes.
KeywordsVolatile Compound Ethanol Production High Resolution Mass Spectrometry Isotope Dilution Method Chemical Noise
Unable to display preview. Download preview PDF.
- Corval, M., 1970, Bull. Soc. Chim. France, 8–9: 2871.Google Scholar
- Danchevskaya, M.N. and Torbin, S.N., 1984, Intern. T. Mass Spectrom. Ion Proc., 56: 251.Google Scholar
- De Leenheer, A.P., Lefevere, M.F., Lanbert, W.E. and Colmet, E. S., 1985, Adv. in Clin. Chem., 24: 111.Google Scholar
- Kolb, B., 1980, “Applied headspace gas chromatography”, Heyden & Sons Ltd. McLafferty, F.W, 1983, “Tandem mass spectrometry”, Wiley, N.Y..Google Scholar
- Veith, H.J., 1978, Organic Mass Spectrom., 13(5): 280.Google Scholar