Utilization of a Monoclonal Antibody to Study in Vitro Phosphorylation of a Low Km Phosphodiesterase
The study of cyclic nucleotide phosphodiesterases in tissues is complicated by the presence of isozymes that differ in terms of selective substrate specificity, substrate affinity, and regulatory and physical properties (for review see Beavo et al., 1982 or Wells and Hardman, 1977). Because the phosphodiesterase isozymes are trace cellular proteins which require several thousand-fold purification to apparent homogeneity, the isolation of these isozymes is very difficult. Isozyme specific probes are needed to study the enzymes in crude systems.
KeywordsDependent Protein Kinase Bovine Heart Phosphodiesterase Activity Cyclic Nucleotide Phosphodiesterase cAMP Phosphodiesterase
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