Protein Sequencing and Covalent Processing
To understand the control of cellular processes at the molecular level, it is important to ascertain the molecular anatomy of constituent receptors, transducers, messengers and enzymes. Although an ultimate goal of such studies is to visualize the three-dimensional interactions of these complex proteins with their regulators and their targets, this level of understanding is only available among a small number of proteins that have been crystallized and analyzed by X-ray diffraction techniques. Amino acid sequence information is available in a much larger number of cases, as determined either by protein chemistry or by analysis of the corresponding DNA. At this level these findings may provide knowledge of the specific location of binding sites within the linear sequences, information concerning modes of regulation (e.g. by phosphorylation or by limited proteolysis), understanding of the domain or subunit sub- structural arrangements, and an inkling of the evolutionary history in their background.
KeywordsProsthetic Group Limited Proteolysis Edman Degradation Zymogen Activation Amino Acid Sequence Information
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