Role of Cytoplasmic pH for Stimulus-Induced CA2+ Mobilization in Human Platelets
Stimulation of human platelets by thrombin resulted in cytoplasmic alkalinization due to activation of Na+/H+ exchange. Inhibition of this Na+/H+ exchange suppressed the thrombin-induced Ca2+ mobilization. Artificial cytoplasmic alkalinization, on the other hand, markedly enhanced the stimulus-induced rises in the cytosolic free Ca2+ concentration. It is concluded that activation of Na+/H+ exchange is needed for Ca2+ mobilization and thus constitutes an essential step in platelet activation.
KeywordsHuman Platelet Inositol Trisphosphate Platelet Suspension Dependent Calcium Channel Cytoplasmic Free Calcium
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