Guanine Nucleotide Regulatory Protein Couples Angiotensin II Receptors to Phospholipase C in Mesangial Cells

  • Josef Pfeilschifter
Part of the NATO ASI Series book series (NSSA, volume 135)


Recently I have shown that vasoconstrictive hormones, such as angiotensin II, vasopressin and norepinephrine, caused a rapid hydrolysis of phosphatidylinositol 4, 5-bisphosphate (PIP2) in rat mesangial cells with subsequent formation of diacylglycerol (DG) and inositoltrisphosphate (IP3) (1, 2). The activation of phospholipase C, which accounted for this degradation of PIP2 further leads to an increased synthesis of prostaglandins (PG). The present study was carried out to gain information about the nature of the link between the occupation of the angiotensin II receptor and the activation of phospholipase C in mesangial cells.


Mesangial Cell Pertussis Toxin Inositol Phosphate PGE2 Synthesis Rapid Hydrolysis 


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  1. 1.
    Pfeilschifter, J., Kurtz, A. & Bauer, C.,(1984) Biochem. J. 223, 855–859PubMedGoogle Scholar
  2. 2.
    Pfeilschifter, J., Kurtz, A. & Bauer, C.,(1986) Biochem. J. 234, 125–130PubMedGoogle Scholar
  3. 3.
    Pfeilschifter, J. & Bauer, C. (1986) Biochem. J. 236, 289–294PubMedGoogle Scholar
  4. 4.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) J. Biol. Chem. 193, 265–275PubMedGoogle Scholar
  5. 5.
    Cockcroft, S. & Gomperts, B.D. (1985) Nature (London) 314, 534–536CrossRefGoogle Scholar
  6. 6.
    Nakamura, T. & Ui, M. (1985) J. Biol. Chem. 260, 3584–3593PubMedGoogle Scholar
  7. 7.
    Brandt, S.J., Dougherty, R.W., Lapetina, E.G. & Niedel, J.E. (1985) Proc. Natl. Acad. Sci. USA 82, 3277–3280PubMedCrossRefGoogle Scholar
  8. 8.
    Volpi, M., Naccache, P.H., Molski, T.F.P., Shefcyk, J., Huang, C.K., Marsh, M.L., Munoz, J., Becker, E.L. & Sha’afi, R.I. (1985) Proc. Natl. Acad. Sci. USA 82, 2708–2712PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Josef Pfeilschifter
    • 1
  1. 1.Physiologisches Institut der Universität ZürichZürichSwitzerland

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