Phosphoinositide-Hydrolysis in a Stimulated Murine T Cell Clone

  • Berthold Behl
  • Beate Schwinzer
  • Klaus Resch
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 135)


The time-dependent accumulation of inositolphosphates was measured in a Con A and IL 2 stimulated murine alloantigen specific, noncytolytic T cell clone. Con A provoked a sharp increase in the inositol triphosphate (IP3) fraction up to 370% of the control. There was also a response to Con A in the production of IP1. (about 200% of the control) and less in IP2. IL 2 had no significant effect on the level of inositol phosphates, whereas under similar conditions cells showed a strong proliferative response to IL 2. Furthermore we investigated the period of exposure to IL 2, which is necessary for the induction of proliferation. Cells had to be incubated more than 4-6 hours to stimulate cell growth. These data suggest that IL 2 in opposite to Con A provokes a slowly increasing process, by which the IL 2 starved lymphocytes are stimulated to proliferation. This may be due to an induction of the expression of IL 2- receptors by IL 2 itself.


Cell Clone Mixed Lymphocyte Culture Inositol Triphosphate Stimulate Cell Growth Phosphoinositide Hydrolysis 
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  1. 1.
    T. A. Waldmann, The structure, function, and expresssion of interleukinT2 receptors on normal and malignant lymphocytes. Science. 232:727 (1986).PubMedCrossRefGoogle Scholar
  2. 2.
    J. B. Imboden and J. D. Stobo, Transmembrane signalling bv the T cell antigen receptor. J. Exp. Med. 161:446 (1985).PubMedCrossRefGoogle Scholar
  3. 3.
    M. V. Taylor, J. C. Metcalfe, T. R. Hesketh, G. A. Smith and J. P. Moore, Mitogens increase phosphorylation of phosphoinositides. Nature. 312:462 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    T. R. Hesketh, J. P. Moore, D. H. Morris, M. V. Taylor, J. Rogers, G. A. Smith and J. C. Metcalfe, A common sequence of calcium and pH signals in the mitogenic stimulation of eukaryotic cells. Nature. 313:481 (1985).PubMedCrossRefGoogle Scholar
  5. 5.
    M. Kohno, S. Kuwata, Y. Namba and M. Hanaoka, Interleukin 2 induces rapid phosphorylation in murine T-lymphocytes. FEBS. 198: 33 (1986).CrossRefGoogle Scholar
  6. 6.
    T. Ishii, K. Sugamura, M. Nakamura and Y. Hinuma, Interleukin 2 (IL 2) rapidly induces phosphorylation of a cellular protein, pp67, in an IL 2 dependent murine cell line. Biochem. Biophys. Res. Comm. 135:487 (1986).PubMedCrossRefGoogle Scholar
  7. 7.
    G. N. Gaulton and D. D. Eardley, Interleukin 2-dependent phosphorylation of interleukin 2 receptors and other T cell membrane proteins. J. Immunol. 136:2470 (1986).PubMedGoogle Scholar
  8. 8.
    W. L. Farrar and W. B. Anderson, Interleukin 2 stimulation of protein kinase C plasma membrane association. Nature. 315:233 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    G. B. Mills, D. J. Stewart, A. Mellors and E. W. Gelfand, Interleukin 2 does not induce phosphatidylinositol hydrolysis in activated T cells. J. Immunol. 136:3019 (1986).PubMedGoogle Scholar
  10. 10.
    J. M. Depper, W. J. Leonard, C. Drogula, M. Krönke, T. A. Waldmann and W. C. Greene, Interleukin 2 (IL-2) augments transcription of the IL-2 receptor gene. Proc. Natl. Acad. Sei. USA. 82:4230 (1985)CrossRefGoogle Scholar
  11. 11.
    J. Weiß, B. Schwinzer, H. Kirchner, D. Gemsa and S. Resch, Effects of Cyclosporin A on Functions of Specific Murine T Cell Clones: Inhibition of Proliferation, Lymphokine secretion and Cytotoxicity. Immunobiol. 171:234 (1986).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Berthold Behl
    • 1
  • Beate Schwinzer
    • 1
  • Klaus Resch
    • 1
  1. 1.Division of Molecular Pharmacology, Department of Pharmacology and ToxicologyMedical School HannoverHannover 61Germany

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