Phosphoinositide-Hydrolysis in a Stimulated Murine T Cell Clone
The time-dependent accumulation of inositolphosphates was measured in a Con A and IL 2 stimulated murine alloantigen specific, noncytolytic T cell clone. Con A provoked a sharp increase in the inositol triphosphate (IP3) fraction up to 370% of the control. There was also a response to Con A in the production of IP1. (about 200% of the control) and less in IP2. IL 2 had no significant effect on the level of inositol phosphates, whereas under similar conditions cells showed a strong proliferative response to IL 2. Furthermore we investigated the period of exposure to IL 2, which is necessary for the induction of proliferation. Cells had to be incubated more than 4-6 hours to stimulate cell growth. These data suggest that IL 2 in opposite to Con A provokes a slowly increasing process, by which the IL 2 starved lymphocytes are stimulated to proliferation. This may be due to an induction of the expression of IL 2- receptors by IL 2 itself.
KeywordsCell Clone Mixed Lymphocyte Culture Inositol Triphosphate Stimulate Cell Growth Phosphoinositide Hydrolysis
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