Advertisement

The Role of Phosphorylation in the Regulation of Acetyl-CoA Carboxylase Activity by Insulin and other Hormones

  • Andrew C. Borthwick
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 135)

Abstract

Insulin stimulates fatty acid synthesis in adipose and other tissues by increasing acetyl-CoA carboxylase activity. 1,2,3,4,5 epididymal fat cells and liver cells this activation is associated with increased phosphorylation of the enzyme at specific sites, particularly within a peptide designated the I-peptide. 6,7,8,9 Inhibition of fatty acid synthesis by hormones such as adrenaline and glucagon involves decreases in acetyl-CoA carboxylase activity. Inhibition is also associated with increased phosphorylation of the enzyme however at sites distinct from those found after insulin treatment. There is good evidence that the adrenaline and glucagon stimulated phosphorylation of the enzyme is carried out by cyclic AMP- dependent protein kinase 10,8,11,12 whereas the insulin stimulated phosphorylation is carried out by an as yet uncharacterised cyclic AMP-independent protein kinase.

Keywords

Fatty Acid Synthesis Concentrate Extract Pyruvate Carboxylase Potassium Citrate Fatty Acid Synthetase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A.P. Halestrap and R.M. Denton, Hormonal regulation of adipose tissue acetyl-CoA carboxylase by changes in the polymeric state of the enzyme, Biochem. J. 142:365 (1974).PubMedGoogle Scholar
  2. 2.
    A.L. Witters, D. Moriarty, and D.B. Martin, Regulation of hepatic acetyl-CoA carboxylase by insulin and glucagon, Biol. Chem. 254:6644, (1979).Google Scholar
  3. 3.
    J.G. McCormack and R.M. Denton, Evidence that fatty acid synthesis in the intrascapular brown adipose tissue of cold adapted rats is increased in vivo by insulin, Biochem. J. 166:627, (1977).PubMedGoogle Scholar
  4. 4.
    K.F. Beuchler, A.C. Beynen and M.J.H. Geelan, Studies on the assay, activity and sedimentation behaviour of acetyl-CoA carboxylase from isolated hepatocytes incubated with insulin and glucagon, Biochem. J. 221:869, (1984).Google Scholar
  5. 5.
    M. R Munday and D.H. Williamson, Effects of starvation, insulin or prolactin deficiency on the activity of acetyl-CoA carboxylase in mammary gland and liver of lactating rats, FEBS Lett. 138:285 (1982).PubMedCrossRefGoogle Scholar
  6. 6.
    R.W. Brownsey, W.A. Hughes, R.M. Denton and R.J. Mayer, Demon stration of the phosphorylation of acetyl-CoA carboxylase within intact rat epididymal fat cells, Biochem. J. 168:441, (1977).PubMedGoogle Scholar
  7. 7.
    R.W. Brownsey and R.M. Denton, Evidence that insulin activates acetyl-CoA carboxylase by increased phosphorylation of a specific site, Biochem. 202:77, (1982)Google Scholar
  8. 8.
    L.A. Witters, J.P. Tipper and G.W. Bacon, Stimulation of site- specific phosphorylation and acetyl-CoA carboxylase by insulin and epinephrine, J. Biol. Chem. 258:5643, (1983).PubMedGoogle Scholar
  9. 9.
    R. Holland and D.G. Hardie, Both insulin and epidermal growth factor stimulates fatty acid synthesis and increase phosphorylation of acetyl-CoA carboxylase and ATP-citrate lyase in isolated hepatocytes, FEBS Lett. 181:308, (1985).PubMedCrossRefGoogle Scholar
  10. 10.
    R.W. Brownsey, W.H. Hughes, and R.M. Denton, Adrenaline and the regulation of acetyl-CoA carboxylase in rat epididymal adipose tissue. Inactivation of the enzyme is associated with phosphorylation and can be reversed by dephosphorylation, Biochem. J. 184:22 (1979).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Andrew C. Borthwick
    • 1
  1. 1.Department of BiochemistryUniversity of BristolBristolUK

Personalised recommendations