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Protein Kinase C of Intestinal Epithelium: Its Role in the Control of Ionic Transport

  • Pedro S. Lazo
  • Gloria Velasco
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 135)

Summary

Protein kinase C activity has been identified in rat and rabbit small intestine epithelial cells. When the crude cytosolic fraction was assayed, a Ca activated protein kinase activity which was independent of any exogenously added phospholipid was identified. Protein kinase C activity was resolved from other protein kinase activities by ion exchange chromatography. Phosphatidylserine was required for protein kinase C to be active. The activation of protein kinase C was a function of phosphatidylserine concentrations up to 50 μg/ml. In the presence of phosphatidylserine and diolein, the Ka for activation by Ca was 10-7 M. The phorbol ester TPA, used at 1–5 ng/ml concentrations, substituted for diacylglycerol in activating protein kinase C. The results are dicussed within a body of evidence which indicates that protein kinase C is implicated in the control of the ionic transport in the intestinal epithelium.

Keywords

Protein Kinase Ionic Transport Intestinal Epithelium Cytosolic Fraction DEAE Cellulose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

PKC

protein kinase C

IP3

inositol 1,4,5 trisphosphate

PS

phosphatidylserine

PI

phosphatidylinositol

PC

phosphatidylcholine

TPA

12-tetradecanoylphorbol-13 acetate

EGTA

ethylene glycol bid (β-aminoethylether)-N,N,N′ N′ tetraacetic acid

EDTA

ethylenediaminetetracetic acid.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Pedro S. Lazo
    • 1
  • Gloria Velasco
    • 1
  1. 1.Departamento de BioquimicaUniversidad de OviedoOviedoSpain

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