Determination of Phosphates in Cardiac Troponin by Phosphate Analysis and Phosphoserine Modification
Troponin (Tn) of high endogenous phosphate content (ca. 3 mol phosphate/mol protein) was isolated from beaf heart. Following separation of Tn into its subunits Tn T, Tn I and Tn C by reverse phase HPLC a phosphate content of about 1.4 mol phosphate/mol protein is found in the Tn T and the Tn I subunits, respectively. These results show, that cardiac Tn contains at least four endogenous phosphorylation sites.
KeywordsPhosphorylation Site Reverse Phase HPLC Cardiac Troponin Phosphate Content Phosphorylase Kinase
Unable to display preview. Download preview PDF.
- Beier, N., 1983, PHD.-thesis, Ruhr-University, BochumGoogle Scholar
- Burtnick, L. D., McCubbin, W. D. and Kay, C. M., 1975, The isolation and characterization of the tropomyosin binding component (Tn T) of bovine cardiac troponin. Can. J. Biochem., 54:546Google Scholar
- Crabb, J. W. and Heilmeyer, L. M. G.,jr., 1984, High performance liquid chromatography purification and structural characterization of the subunits of rabbit muscle Phosphorylase kinase. Biol. Chem., 259:6346Google Scholar
- Meyer, H. E., Swiderek, K., Hoffmann-Posorske, E., Korte, H. and Heilmeyer, L. M. G., jr., 1986a, Quantitative determination of phosphoserine by PTC-amino acid analysis after modification to S-ethyl-cysteine. Application for picomolar amounts of peptides and proteins, J. Chromat., submittedGoogle Scholar
- Stull, J. T., 1980, in: Adv. in Cyclic Nucl. Res., eds, P. Greengard and G. A. Robison, Raven Press, New YorkGoogle Scholar
- Tsukui, R. and Ebashi, S., 1973, Cardiac troponin, J. Biol. Chem., 73:1119Google Scholar