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Determination of Phosphates in Cardiac Troponin by Phosphate Analysis and Phosphoserine Modification

  • Kristine Swiderek
  • Kornelia Jaquet
  • Helmut E. Meyer
  • Ludwig M. G. HeilmeyerJr.
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 135)

Abstract

Troponin (Tn) of high endogenous phosphate content (ca. 3 mol phosphate/mol protein) was isolated from beaf heart. Following separation of Tn into its subunits Tn T, Tn I and Tn C by reverse phase HPLC a phosphate content of about 1.4 mol phosphate/mol protein is found in the Tn T and the Tn I subunits, respectively. These results show, that cardiac Tn contains at least four endogenous phosphorylation sites.

Keywords

Phosphorylation Site Reverse Phase HPLC Cardiac Troponin Phosphate Content Phosphorylase Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Kristine Swiderek
    • 1
  • Kornelia Jaquet
    • 1
  • Helmut E. Meyer
    • 1
  • Ludwig M. G. HeilmeyerJr.
    • 1
  1. 1.Institut für Physiologische Chemie Abt. Biochemie Supramolekularer SystemeRuhr-Universität BochumGermany

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