Fluorescence is widely used in biochemistry and cell biology because this method offers many advantages. Fluorescent labels often bind specifically to one type of molecules or ions. The concentration of the fluorescently labeled molecules or ions can be determined without interference from other molecules. Concentrations in the nanomolar range or below can be accurately determined. Advanced techniques and instruments have been developed by which the mobility and turnover of fluorescently labeled molecules can be observed in a test tube or in living cells.1 Rotation and diffusion of fluorescent molecules can be measured and the locations where fluorescent molecules assemble or are transported in living cells, can be determined.
KeywordsLife Time Fluorescent Label Excited Molecule Fluorescent Molecule Nanomolar Range
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