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Fluorescent Probes

  • Norma Selve
  • Elke Schröer
  • Klaus Ruhnau
  • Albrecht Wegner
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 135)

Abstract

Fluorescence is widely used in biochemistry and cell biology because this method offers many advantages. Fluorescent labels often bind specifically to one type of molecules or ions. The concentration of the fluorescently labeled molecules or ions can be determined without interference from other molecules. Concentrations in the nanomolar range or below can be accurately determined. Advanced techniques and instruments have been developed by which the mobility and turnover of fluorescently labeled molecules can be observed in a test tube or in living cells.1 Rotation and diffusion of fluorescent molecules can be measured and the locations where fluorescent molecules assemble or are transported in living cells, can be determined.

Keywords

Life Time Fluorescent Label Excited Molecule Fluorescent Molecule Nanomolar Range 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Norma Selve
    • 1
  • Elke Schröer
    • 1
  • Klaus Ruhnau
    • 1
  • Albrecht Wegner
    • 1
  1. 1.Institut für Physiologische Chemie IRuhr-Universität BochumBochumFederal Republic Germany

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