Cloning and molecular characterization of monoclonal antibody-defined ovarian tumour antigens
In an effort to develop additional treatments and improved methods for early detection of ovarian cancers a number of monoclonal antibodies (MAbs) have been generated against cell surface antigens of epithelial ovarian cancer (EOC). Therapies using these reagents to target ovarian cancers have so far been disappointing. Identifying the antigens recognized by the antibody may be important for improving the targeting of antibody based therapy. For example, detailed molecular analysis of the polymorphic epithelial mucin gene, MUCl, has shown that differential glycosylation of a tandem repeat unit is responsible for the appearance of novel epitopes in breast, ovarian and other adenocarcinomas . This information has opened up new possibilities for immunotherapy with mucins since these structures are highly immunogenic and are relatively tumour specific. The encouraging results of such studies prompted us to undertake the cloning of the genes for other MAb defined tumour antigens based on an expression cloning technique . In this chapter we briefly summarize the important characteristics of the genes cloned so far and discuss their possible function in ovarian cancer.
KeywordsOvarian Cancer Epithelial Ovarian Cancer Folate Receptor Immunoglobulin Superfamily Epithelial Ovarian Cancer Patient
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