Abstract
Properties of purified proteinase inhibitors from the small-seeded grains, finger millet, sorghum, pearl millet, Italian millet, and barnyard millet are compared. The changes in the levels of inhibitory activities during germination and plant growth of finger millet and barnyard millet are discussed. The action of millet proteinase inhibitors on human and other animal pancreatic enzymes is detailed. The role of substrates in determining the magnitude of the inhibition of proteolytic enzymes by the millet inhibitors is emphasized.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Boisen, S. and Djurtoft, R. (1981). Trypsin inhibitor from wheat endosperm: Purification and characterization. Cereal Chem., 58, 460–463.
Boisen, S. and Djurtoft, R. (1981 a). Trypsin inhbitor from rye endosperm: Purification and properties. Cereal Chem., 58, 194–198.
Campos, F. A. P. and Richardson, M. (1983). The complete amino acid sequence of the bifunctional alpha-amylase/trypsin inhibitor from seeds of ragi (Indian finger millet, Eleusine coracana, Gaertn). FEBS Letters, 167, 221–225.
Chandrasekher, G. and Pattabiraman, T. N. (1982). Natural plant enzyme inhibitors: Isolation and characterization of two trypsin inhibitors from bajra (Pennisetum typhoideum). Indian J. Biochem. Biophys., 19, 1–7
Chandrasekher, G., Raju, D. S. and Pattabiraman, T. N. (1982). Natural plant enzyme inhibitors. Protease inhibitors in millets. J. Sci. Food Agric, 33, 447–450.
Harish Kumar, P. M., Virupaksha, T. K. and Vithayathil, P. J. (1978). Sorghum protease inhbitors. Purification and some biochemical properties. Intl. J. Peptide Protein Res., 12, 185–196.
Johnson, R., Mitchell, H. L., Wassom, C. E. and Reeck, R. (1980). Comparison of trypsin inhibitors from several types of corn. Phytochemistry, 19, 2749–2750.
Kashlan, N. and Richardson, M. (1981). The complete amino acid sequence of a major wheat protein inhibitor of amylase. Phytochemistry, 20, 1781–1784.
Laskowski, M. and Laskowski, M., Jr. (1954). Naturally occurring trypsin inhibitors. In “Advances in Protein Chemistry”, M. L. Anson, K. Bailey and J. T. Edsall, eds., Academic Press, New York, Vol. 9, pp. 203–242.
Liener, I. E. (1979). Protease inhibitors and lectins. Intl. Rev. Biochem., 27, 97–122.
Maki, Z., Tashiro, M., Sugihara, N. and Kanamori, M. (1980). Doubleheaded nature of a trypsin inhibitor from rice bran. Agric. Biol. Chem. (Japan), 44, 953–955.
Mundy, J., Hejgaard, J. and Svendsen, I. (1984). Characterization of a bifunctional wheat inhibitor of endogenous alpha-amylase and subtilisin. FEBS Letters, 167, 210–214.
Odani, S., Koide, T. and Ono, T. (1982). Sequence homology between barley trypsin inhibitor and wheat alpha-amylase inhibitors. FEBS Letters, 141, 279–282.
Odani, S., Koide, T., Ono, T. and Ohnishi, K. (1983). Structural relationship between barley (Hordeum vulgare) trypsin inhibitor and castor bean (Ricinus communis) storage protein. Biochem. J., 213, 543–545.
Price, M. L. and Butler, L. G. (1980). Treatment of sorghum grains that reduce the assayable tannin content and their effect on the nutritional value of the grain. In “Polyphenols in Cereals and Legumes”, J. H. Hulse, ed., International Development Research Centre, Ottawa, Canada, pp. 39–42.
Richardson, M. (1981). Protein inhibitors of enzymes. Food Chemistry, 6, 235–253.
Shivaraj, B. and Pattabiraman, T. N. (1981). Natural plant enzyme inhibitors. Characterization of an unusual alpha-amylase/trypsin inhibitor from ragi (Ekeusine coracana, Geartn). Biochem. J., 193, 29–36.
Shivaraj, B., Rao, H. N. and Pattabiraman, T. N. (1982). Natural plant enzyme inhibitors. Isolation of a trypsin/alpha-amylase inhibitor and a chymotrypsin/trypsin inhibitor from ragi (Eleusine coracana) grain by affinity chromatography and study of their properties. J. Sci. Food Agric., 33, 1080–1091.
Udupa, S. L., Khanum, F. and Pattabiraman, T. N. (1984). Comparative study of the action of cereal protease inhibitors on human, bovine and porcine pancreatic enzymes. J. Indian Inst. Sci. 65(C), 1–9.
Udupa, S. L. and Pattabiraman, T. N. (1985). Isolation and characterization of a trypsin/chymotrypsin inhibitor from the millet Echinocloa fruneutacea. J. Agric. Food Chem., 33, 642–646.
Veerabhadrappa, P. S., Manjunath, N. H. and Virupaksh, T. K. (1978). Proteinase inhibitor of finger millets (Eluesine corcana Gaertn). J. Sci. Food Agric, 29, 353–358.
Vidyavathi, U., Shivaraj, B. and Pattabiraman, T. N. (1983). Proteases in germinating finger millet (Eleusine coracana) seeds. J. Bioscience, 5, 219–224.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Pattabiraman, T.N. (1986). Trypsin/Chymotrypsin Inhibitors from Millets. In: Friedman, M. (eds) Nutritional and Toxicological Significance of Enzyme Inhibitors in Foods. Advances in Experimental Medicine and Biology, vol 199. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0022-0_25
Download citation
DOI: https://doi.org/10.1007/978-1-4757-0022-0_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-0024-4
Online ISBN: 978-1-4757-0022-0
eBook Packages: Springer Book Archive