Regulation of Proteinase Inhibitor Genes in Food Plants

  • Willis E. Brown
  • John S. Graham
  • Jong S. Lee
  • Clarence A. Ryan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 199)


Proteinase inhibitor proteins in the families Solanaceae, Leguminosae and Graminae are stored in seeds and tubers and are also found to accumulate in leaves in response to pest attacks. The isolation of the proteinase Inhibitor I cDNA and its gene from tomato has provided information concerning proteinase inhibitor synthesis, processing and compartmentation in leaves under pest attacks. Strategies are now being developed using proteinase inhibitor genes, to genetically engineer the quantity and quality of these potentially defensive and highly nutritional proteins of important crop plants.


Tomato Leave Trypsin Inhibitor Activity Proteinase Inhibitor Gene Pest Attack Transit Sequence 
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  1. 1.
    T.B. Osborne, and L.B. Mendel, The use of soybeans as foods, J. Biol. Chem. 32 369 (1917).Google Scholar
  2. 2.
    J.R. Whitaker, and R.E. Feeney, Enzyme inhibitors in foods, in Toxicants Occurring Naturally in Foods, National Academy of Science, Washington, D.C. pp. 276–298 (1973).Google Scholar
  3. 3.
    R.R. Rackis, Protease inhibitors: physiological properties and nutritional significance, in: “Antinutrients and Natural Toxicants in Foods,” R.L. Ory, ed. Food and Nutrition Press Inc. Westport Comm. pp. 203–238 (1981).Google Scholar
  4. 4.
    G.M. Green, W.H. Levan and R.A. Liddle, Regulation of plasma cholecystokinin and pancreatic function by substrates and inhibitors of pancreatic proteases. This volume.Google Scholar
  5. 5.
    J.J. Rackis, E.G. Baker and W.J. Wolf, Inactivation and removal of protease inhibitors from plant foodsGoogle Scholar
  6. 6.
    D. Gallaher and B.O. Schneeman, Nutritional and metabolic response to plant inhibitors of digestive enzymes, in Nutritional and toxicological aspects of food safety, M. Friedman ed. Plenum Press, N.Y. Vol 177, pp. 299–320 (1984).CrossRefGoogle Scholar
  7. 7.
    G. Pearce, J. McGinnis, and C.A. Ryan, Utilization by chicks of half-cystine from native and denatured proteinase inhibitor protein from potatoes, Proc. Soc. Expt. Biol. Med. 160:180 (1979).Google Scholar
  8. 8.
    C.A. Ryan, Proteolytic enzymes and their inhibitors in plants, Ann. Rev. Plant Physiol. 24:173 (1973).CrossRefGoogle Scholar
  9. 9.
    M. Richardson, The proteinase inhibitors of plants and microorganisms. Photochemistry 16:159 (1977).CrossRefGoogle Scholar
  10. 10.
    J. Mikola and M. Kirsi, Differences between endospermal and embryonyl trypsin inhibitors in barley, wheat and rye, Acta. Chem. Seond. 26:787 (1972).CrossRefGoogle Scholar
  11. 11.
    R.B. Goldberg, R.L. Fischer, J.L. Harada, D. Jafukos, and J.K. Okamuro, Organization of soybean seed protein genes and their flanking regions, in: “Structure and Function of Plant Genomes,” O. Ciferri and L. Dure, eds. Plenum Press, pp. 37–45 (1983).Google Scholar
  12. 12.
    G. Pearce and C.A. Ryan, in preparation.Google Scholar
  13. 13.
    T.R. Green and C.A. Ryan, Wound-induced proteinase inhibitor in plant leaves: A possible defense mechanism against insects, Science 175:776 (1972).CrossRefGoogle Scholar
  14. 14.
    C.A. Ryan, Proteinase inhibitors in plant leaves: A biochemical model for pest-induced natural plant protection, Trends in Biochemical Sci. 5:148 (1978).CrossRefGoogle Scholar
  15. 15.
    W.E. Brown, K. Takio, K. Titani, and C.A. Ryan, Wound-induced trypsin inhibitor in alfalfa leaves: Identity as a member of the Bowman-Birk inhibitor family, Biochemistry 24:2105 (1985).CrossRefGoogle Scholar
  16. 16.
    C.A. Ryan, P. Bishop, G. Pearce, and M. Walker-Simmons, Pectic fragments regulate the expression of proteinase inhibitor genes in plants, UCLA Symposium, in: “Cellular and Molecular Biology of Plant Stress,” J.L. Key, and T. Kosuge, eds. Alan R. Liss Inc., (1985).Google Scholar
  17. 17.
    J.C. Melville and C.A. Ryan, Chymotrypsin inhibitor from potatoes: Large scale preparation and characterization of its subunit components, M. Biol. Chem. 247 3445 (1972).Google Scholar
  18. 18.
    J. Bryant, T. Green, T. Gurusadaaiah, and C.A. Ryan, Proteinase inhibitor II from potatoes: Isolation and characterization of the isoinhibitor subunits, Biochemistry 15:3418 (1976).22.CrossRefGoogle Scholar
  19. 19.
    G.M. Hass and C.A. Ryan, Carboxypeptidase inhibitor from potatoes, in: Methods in Enzymology, “Proteolytic Enzymes, Part C” L. Lorand, ed. Academic Press (1981).Google Scholar
  20. 20.
    C.A. Ryan, P. Bishop, G. Pearce, A.G. Darvill, M. McNeil, and P. Albersheim, A sycamore cell wall polysaccharide and a chemically related tomato leaf polysaccharide possess similar proteinase inhibitor-inducing activities, Plant Physiol. 68:616 (1981).CrossRefGoogle Scholar
  21. 21.
    P. Bishop, D.J. Makus, G. Pearce, and C.A. Ryan, Proteinase inhibitor-inducing factor activity in tomato leaves resides in oligosaccharides enzymically released from cell walls, Proc. Natl. Acad. Sci. 78:3536 (1981).CrossRefGoogle Scholar
  22. 22.
    P. Bishop, G. Pearce, J.E. Bryant, and C.A. Ryan, Isolation and characterization of the proteinase inhibitor-inducing factor from tomato leaves: Identity and activity of poly- and oligogalacturonide fragments, J. Biol. Chem. 259:13172 (1984).Google Scholar
  23. 23.
    G. Plunkett, D.F. Senear, G. Zuroske, and C.A. Ryan, Proteinase Inhibitor I and II from Leaves of Wounded Tomato Plants: Purification and Properties, Arch. Biochem. Biophys. 213 463 (1982).CrossRefGoogle Scholar
  24. 24.
    J. Graham, G. Pearce, J. Merryweather, K. Titani, and C.A. Ryan, Wound-induced proteinase inhibitor mRNA from tomato leaves: I. The cDNA-deduced primary sequence of pre-inhibitor, J. Biol. Chem. 260:6555 (1985).Google Scholar
  25. 25.
    J. Graham, G. Pearce, J. Merryweather, K. Titani, and C.A. Ryan, Wound-induced proteinase inhibitor mRNA from tomato leaves: II. The cDNA-deduced primary sequence of pre-inhibitor II, J. Biol. Chem. 260:6561 (1985)Google Scholar
  26. 26.
    M. Walker-Simmons and C.A. Ryan, Immunological identification of proteinase Inhibitors I and II in isolated tomato leaf vacuoles, Plant Physiol. 60:61 (1977).CrossRefGoogle Scholar
  27. 27.
    H. Hollander-Czytko, J.K. Andersen and C.A. Ryan, Vacuolar localization of wound-induced carboxypeptidase inhibitor in potato leaves, Plant Physiol. 78:76 (1985).CrossRefGoogle Scholar
  28. 28.
    CE. Nelson and C.A. Ryan, In vitro synthesis of pre-proteins of two vacuolar compartmented proteinase inhibitors that accumulate in leaves of wounded tomato plants, Proc. Natl. Acad. Sci. 77:1975 (1980).CrossRefGoogle Scholar
  29. 29.
    J. Graham and C.A. Ryan, in preparation.Google Scholar
  30. 30.
    G. An, B.D. Watson, S. Stachel, M. Gordon, and E.W. Nester, New cloning vehicles for transformation of higher plants, EMBO J. 4:277 (1985).Google Scholar
  31. 31.
    W. Brown and C.A. Ryan, Isolation and characterization of a wound-induced trypsin inhibitor from alfalfa leaves, Biochemistry 23:3418 (1984).CrossRefGoogle Scholar
  32. 32.
    L. Hadwiger and J.M. Beckman, Chitosan as a component of pea-Fusarium solani interactions, Plant Physiol. 66:205 (1980).CrossRefGoogle Scholar
  33. 33.
    M. Walker-Simmons, L. Hadwiger and C.A. Ryan, Chitosan and pectic polysaccharides both induce accumulation of the antifungal phytoalehin pisatin in pea pods and antinutrient proteinase inhibitors in tomato leaves, Biochem. Biophys. Res. Comm. 110 194 (1983).CrossRefGoogle Scholar
  34. 34.
    S. Norioki and T. Ikenaka, Amino acid sequence of a trypsin-chymotrypsin inhibitor, B-111, of peanut (Arachis hypogea) J. Biochem. 93: 479 (1983).Google Scholar
  35. 35.
    T. Ikenaka, S. Odani and T. Koide, Chemical structure and inhibitory activities of soybean proteinase inhibitors. Bayer-Symposium V “Proteinase Inhibitors” H. Fritz, H. Tschesche, L.J. Greene and E. Truscheit eds. Springer Serlog, pp. 325–343 (1974).Google Scholar
  36. 36.
    R. Hawkes, E. Hiday and J. Gordon, A dot-immunobinding assay for monoclonal and other antibodies. Anal. Biochem. 119:142 (1982).CrossRefGoogle Scholar
  37. 37.
    W. Brown, K. Takio, K. Titani, and C.A. Ryan, Wound-induced trypsin inhibitor in alfalfa leaves: Identity as a member of the Bowman-Birk inhibitor family, Biochemistry 24 2105 (1985).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Willis E. Brown
    • 1
  • John S. Graham
    • 1
  • Jong S. Lee
    • 1
  • Clarence A. Ryan
    • 1
  1. 1.Institute of Biological ChemistryWashington State UniversityPullmanUSA

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