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Purification of Ca2+-ATPase from Rat Pancreatic Acinar Plasma Membranes Using Calmodulin-Affinity Chromatography

  • M. Bridges
  • R. Mahey
  • W. Lau
  • M. Hampong
  • S. Katz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 232)

Abstract

Although a great deal is known about the stimulatory role of calcium in stimulus-secretion coupling, little is known about the mechanism by which intracellular calcium is regulated. The free calcium concentration in the acinar cell cytoplasm is typically between 10−5–10−7 M, in contrast to the much higher levels of calcium (approximately 10−3 M) in the extracellular space (Peterson, 1982). Calcium therefore penetrates into cells continuously down its electrochemical gradient by passive diffusion. In order to maintain a homeostatic environment in the cell and assure proper cell function, free calcium must be continuously removed.

Keywords

ATPase Activity Acinar Cell Pancreatic Acinar Cell Free Calcium Concentration Transport ATPase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • M. Bridges
    • 1
  • R. Mahey
    • 1
  • W. Lau
    • 1
  • M. Hampong
    • 1
  • S. Katz
    • 1
  1. 1.Division of Pharmacology and Toxicology, Faculty of Pharmaceutical SciencesUniversity of British ColumbiaVancouverCanada

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