Purification of Ca2+-ATPase from Rat Pancreatic Acinar Plasma Membranes Using Calmodulin-Affinity Chromatography
Although a great deal is known about the stimulatory role of calcium in stimulus-secretion coupling, little is known about the mechanism by which intracellular calcium is regulated. The free calcium concentration in the acinar cell cytoplasm is typically between 10−5–10−7 M, in contrast to the much higher levels of calcium (approximately 10−3 M) in the extracellular space (Peterson, 1982). Calcium therefore penetrates into cells continuously down its electrochemical gradient by passive diffusion. In order to maintain a homeostatic environment in the cell and assure proper cell function, free calcium must be continuously removed.
KeywordsATPase Activity Acinar Cell Pancreatic Acinar Cell Free Calcium Concentration Transport ATPase
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