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Cellular Ca2+ Dyshomeostasis in the Liver in Endotoxic Shock

  • Mohammed M. Sayeed
  • Subir R. Maitra
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 232)

Abstract

Cellular Ca2+ homeostasis is understood to be maintained by three membrane systems viz. the plasma membrane, the inner mitochondrial and the endoplasmic reticular membranes. Each of these membranes possesses distinctive Ca2+ transport processes by means of which it regulates cytosolic Ca2+ concentration.1 While Ca2+ mobilization into the cytosolic compartment subsequent to cell activation plays an important role in the elicitation of various normal cellular responses, an inappropriate elevation of intracellular Ca2+ causes cell damage. Although the precise mechanism of cell damage remains to be understood, it could result from Ca2+ induced activation of cytolytic processes2,3 and altered regulation of enzymes required for cellular metabolism and ion transport.4,5 Several studies have implicated altered cellular Ca2+ regulation in cell damage within various organ systems of animals in septic and endotoxic shock.6,7,8 In our laboratory, we have evaluated alterations in the regulation of hepatic intracellular Ca2+ in endotoxic animals. These evaluations included measurements of (1) cellular Ca2+ efflux and its modulation by norepin- ephrine, (2) Ca2+ uptake by endoplasmic reticulum, and (3) cytoplasmic exchangeable Ca2+. In addition, cytosolic free Ca2+ concentrations were measured under basal and hormone stimulated conditions.

Keywords

Endoplasmic Reticular Membrane Liver Slice Endotoxic Shock Washout Curve Pyruvate Carboxylation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Mohammed M. Sayeed
    • 1
  • Subir R. Maitra
    • 1
  1. 1.Department of PhysiologyLoyola University Stritch School of MedicineMaywoodUSA

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