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Sodium-Calcium Exchange in Platelet Plasma Membrane Vesicles

  • Appavoo Rengasamy
  • Harold Feinberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 232)

Abstract

Unstimulated platelets maintain submicromolar concentration of cytosolic Ca2+ (~100 μM) and a steep plasma membrane Ca2+ gradient. Since passive inward diffusion of Ca2+ will cause platelet activation, mechanisms in addition to Ca2+ sequestration by endoplasmic reticulum must exist to remove Ca2+ from cytosol. In other tissues low cytosolic Ca2+ is achieved, in part, by plasma membrane ATP-dependent Ca2+ efflux1,2 and Na+-Ca2+ exchange3,4. Although platelets possess Ca2+-Mg2+-ATPase activity, the available evidence suggests that this enzyme is present only in the inner membranes5,6. Whether Na+-Ca2+ exchange activity occurs in platelets is not known. We investigated the involvement of Na+-Ca2+ exchange in this process. This study establishes that platelet membranes enriched in plasma membrane markers exhibited Na+-Ca2+ exchange activity.

Keywords

Calcium Uptake Uptake Medium Platelet Membrane Adenosine Triphosphatase Submicromolar Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Appavoo Rengasamy
    • 1
  • Harold Feinberg
    • 1
  1. 1.Department of PharmacologyUniversity of Illinois College of Medicine at ChicagoChicagoUSA

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