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Oxygen Binding in Artemia Hemolymph

  • G. H. Wolf
  • B. De Wachter
  • F. Hens
  • A. Van der Linden
Part of the NATO ASI Series book series (NSSA, volume 174)

Abstract

Two Artemia species, i.e. Great Salt Lake (A. franciscana) and Kazakhstan (A. parthenogenetica) were submitted to a stepwise lowering of the partial oxygen pressure until 1 ml 02.L−1 was reached in the saline medium (35%). The increase of hypoxia resulted in the increase of the hemoglobin concentration of the hemolymph. Oxygen binding was determined in small freshly collected hemolymph samples (2 to 4 ul) of preadult females. The oxygen binding curves show an undulating biphasic course. This phenomenon only occurs with Artemia hemolymph and was never noticed when measuring Artemia hemoglobin extracts. Similar curves have been reported [1,2] for the vertebrate sickle cell Hbs, which are sensitive to aggregation, gelation and crystallization effects resulting in biphasic oxygen binding curves. As far as we know, this is the first time that biphasic oxygen binding curves have been registrated for blood solutions containing invertebrate Hbs. Our results reveal that both Artemia species show biphasic oxygen binding curves for hemolymph with and without buffer (pH 8.58) and/or added GTP (3.1 mg.mL−1). Oxygen affinity (p50) and cooperativity (n50) were also determined (25°C).

Keywords

Public Health Molecular Biology Link Function Partial Oxygen Pressure Hemoglobin Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    A. Colosimo, M. Brunori and J. Wymon, Polysteric linkage, J. Mol. Biol. 100:47 (1976).PubMedCrossRefGoogle Scholar
  2. 2.
    J. Wyman, Linked functions and reciprocal effects in hemoglobin: a second look, Adv. Protein Chem. 19:223 (1964).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • G. H. Wolf
    • 1
  • B. De Wachter
    • 1
  • F. Hens
    • 1
  • A. Van der Linden
    • 1
  1. 1.Laboratory of Biochemistry and General Zoology (RUCA)AntwerpenBelgium

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