The Molecular Biology of Artemia Haemoglobins
Haemoglobin is a major protein induced during post-gastrula development of Artemia. Three haemoglobins are formed from the association of two different subunits, and they are expressed differentially during post-gastrula development. These haemoglobins are unusual in that they are of high molecular weight (Mr 260,000) and are composed of two globin chains (Mr 130,000) each of which is a polymer of eight covalently linked myoglobin-like domains . Each polypeptide is encoded by a single large mRNA which may have originated as a result of successive tandem duplication and fusion of a single ancestral globin gene .
KeywordsGlobin Gene Preceding Domain Rabbit Reticulocyte Lysate Globin Chain Derive Amino Acid Sequence
Unable to display preview. Download preview PDF.
- 2.A. M. Manning, G. S. Ting, B. C. Mansfield, C. N. A. Trotman and W. P. Tate, The isolation and faithful translation of Artemia naupliar haemoglobin mRNA, Biochem. Int. 12:715 (1986).Google Scholar
- 3.C. J. Marshall, J. F. Cutfield, C. N. A. Trotman and W. P. Tate, Purification of the haemoglobins I and III from the brine shrimp, Artemia, Biochem. Int. 12:693 (1986).Google Scholar
- 4.T. Maniatis, E. F. Fritsch and J. Sambrook, Electrophoresis of RNA through Gels containing Formaldehyde, in “Molecular Cloning: A Laboratory Manual,” Cold Spring Harbor Laboratory, Cold Spring Harbor (1982).Google Scholar
- 5.D. J. Denhardt, D. Dressler and D. S. Ray, “The Single Stranded DNA Phages,” Cold Spring Harbor Laboratory, Cold Spring Harbor (1978).Google Scholar
- 7.C. Lane and J. Knowland, The injection of mRNA into living cells: The use of frog oocytes for the assay of mRNA in the study of the control of gene expression, in: “The Biochemistry of Animal Development,” Vol. 3; R. Weber, ed., Academic Press, New York (1975).Google Scholar
- 10.T. V. Huynh, R. A. Young and R. W. Davis, Constructing and screening cDNA libraries in λgt10 and λgt11, in “DNA Cloning: A Practical Approach,” Vol. 1, D. Glover, ed., IRL Press, Oxford (1985).Google Scholar
- 17.C. J. Marshall J. F. Cutfield, C. N. A. Trotman and W. P. Tate, An analysis of Artemia haemoglobins by comparison of immunoreactivities, Biochem. Int. 15:925 (1987).Google Scholar
- 18.A. A. Reyes and R. B. Wallace, in “Gentic Engineering: Principles and Methods,” Vol. 6., Plenum, New York (1984).Google Scholar
- 19.A. M. Manning, C. N. A. Trotman and W. P. Tate, Isolation of cDNA clones for the haemoglobins of the brine shrimp, Artemia, Proc. Univ. Otago. Med. Sch. 66:9 (1988).Google Scholar
- 20.K. De Smet, M-L. Van Hauwaert, L. Moens and J. Van Beeumen, The structure of Artemia sp. haemoglobins II. A comparison of the structural units composing the Artemia sp. globin chains, in “Artemia Research and its Applications,” Vol. 2, W. Decleir, L. Moens, H. Siegers, E. Jaspers and P. Sorgeloos, eds., Universa Press, Wetteren (1987).Google Scholar