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Tubulin Synthesis and Microtubule Organization in Artemia

  • Thomas H. MacRae
  • Elizabeth J. Campbell
  • Carrie M. Langdon
Part of the NATO ASI Series book series (NSSA, volume 174)

Abstract

Many aspects of eukaryotic cell function and structural organization depend on the cytoskeleton, an interconnected array of filamentous elements including microtubules, microfilaments and intermediate filaments. The main structural protein of microtubules is tubulin, a heterodimer of α- and β- tubulin. The tubulins are in most cases the products of small gene families [1–3], giving rise to collections of isotubulins. The best characterized tubulin gene family occurs in the chicken where several, if not all, of the α- and β- genes have been cloned and sequenced. At least seven β-tubulin genes encoding six β-tubulin isotypes are present, and except for the β-tubulin gene expressed in erythrocytes, they are relatively highly conserved [2]. Most of the diversity within the chicken β-tubulins exists in a region near the carboxy terminus, with a smaller amount nearer the amino end of the protein. The β- isotypes, based on the variable region sequences and their tissue — specific expression, are divided into families. Each member of the family in the chicken shares an identical or almost identical sequence, even within the variable region, with a β-tubulin gene from human or other vertebrates. The genes of corresponding sequence are expressed in similar tissues from one species to another. The chicken α-tubulin gene family has at least five functional genes, and the five polypeptides they encode are more heterogeneous than those encoded by the β-tubulin genes [3]. The α-tubulins do not readily segregate into conserved classes within the vertebrates, although their major region of heterogeneity is at the carboxy terminus of the protein. In organisms other than the vertebrates, the number and arrangement of the tubulin genes is widely divergent but, like the vertebrate genes, they are subject to developmental regulation and yield isotubulin families of differing complexity [1].

Keywords

Brine Shrimp Neural MAPs Carboxy Terminus Trypanosoma Brucei Tubulin Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Thomas H. MacRae
    • 1
  • Elizabeth J. Campbell
    • 1
  • Carrie M. Langdon
    • 1
  1. 1.Department of BiologyDalhousie UniversityHalifaxCanada

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